{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T08:40:20Z","timestamp":1793090420987,"version":"4.5.0"},"reference-count":68,"publisher":"American Chemical Society (ACS)","license":[{"start":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:00:00Z","timestamp":1793059200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:00:00Z","timestamp":1793059200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:00:00Z","timestamp":1793059200000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/100006151","name":"Basic Energy Sciences","doi-asserted-by":"publisher","award":["DE-FG02-95ER20175"],"award-info":[{"award-number":["DE-FG02-95ER20175"]}],"id":[{"id":"10.13039\/100006151","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006151","name":"Basic Energy Sciences","doi-asserted-by":"publisher","award":["DE-SC0019342"],"award-info":[{"award-number":["DE-SC0019342"]}],"id":[{"id":"10.13039\/100006151","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["5R01GM111097-53"],"award-info":[{"award-number":["5R01GM111097-53"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Philadelphia College of Osteopathic Medicine Division of Research","id":[{"id":"https:\/\/ror.org\/00m9c2804","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Ferredoxin (Fd) from the hyperthermophilic archaeon Pyrococcus furiosus (Pf), Pf-Fd, functions in electron transfer, serving to transfer an electron from various oxidoreductases to an H2-evolving, membrane-bound hydrogenase. The [4Fe-4S] cluster of Pf-Fd is coordinated by three cysteine residues and the carboxylate group of an aspartate residue. Recently, we have found that several such site-differentiated [4Fe-4S]1+ clusters are photoactive, inspiring us to investigate the photoreactivity of the site-differentiated [4Fe-4S]1+ cluster in Pf-Fd. We show here through X-band EPR and 13C\/15N Q-band ENDOR studies that visible-light irradiation of the cyanide-bound [4Fe-4S]1+ cluster of Pf-Fd, Pf-Fd-CN, at 12 K results in photodissociation of the cyanide anion, which geminately recombines only upon annealing to 150 K. In addition, this work revisits our previous CW 13C\/15N ENDOR studies of Pf-Fd-CN with pulsed-ENDOR techniques. Of particular note, we definitively determine the absolute sign of the 13C hyperfine coupling with the PESTRE pulsed-ENDOR protocol and from this draw inferences about Fe valence localization within the cyanide-bound cluster.<\/jats:p>","DOI":"10.1021\/acs.inorgchem.6c04022","type":"journal-article","created":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T03:01:40Z","timestamp":1793070100000},"source":"Crossref","is-referenced-by-count":0,"title":["Cyanide Photodissociation\nfrom the Site-Differentiated\n[4Fe-4S]1+ Cluster of\n                    <i>Pyrococcus furiosus<\/i>\n                    Ferredoxin Characterized by EPR and 13C\/15N ENDOR"],"prefix":"10.1021","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6351-2132","authenticated-orcid":true,"given":"Madeline B.","family":"Ho","sequence":"first","affiliation":[{"name":"Northwestern University , , , ,","place":["Evanston, Illinois, United States, 60208"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7229-0957","authenticated-orcid":true,"given":"Hao","family":"Yang","sequence":"additional","affiliation":[{"name":"Northwestern University , , , ,","place":["Evanston, Illinois, United States, 60208"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1667-6924","authenticated-orcid":true,"suffix":"Jr.","given":"Francis E.","family":"Jenney","sequence":"additional","affiliation":[{"name":"Georgia Campus, Philadelphia College of Osteopathic Medicine , , ,","place":["Suwanee, Georgia, United States, 30024"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0881-8870","authenticated-orcid":true,"given":"Michael W. W.","family":"Adams","sequence":"additional","affiliation":[{"name":"University of Georgia , , , ,","place":["Athens, Georgia, United States, 30602"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3307-2556","authenticated-orcid":true,"given":"Joshua","family":"Telser","sequence":"additional","affiliation":[{"name":"Roosevelt University , , , ,","place":["Chicago, Illinois, United States, 60605"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3100-0746","authenticated-orcid":true,"given":"Brian M.","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Northwestern University , , , ,","place":["Evanston, Illinois, United States, 60208"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,10,27]]},"reference":[{"key":"2026102623013043300_cit1","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1021\/ja002183v","article-title":"S K-edge X-ray absorption studies of tetranuclear iron-sulfur\nclusters: \u03bc-sulfide bonding and its contribution to electron\ndelocalization","volume":"123","author":"Glaser","year":"2001","journal-title":"J. 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Biol.\nChem."},{"key":"2026102623013043300_cit30","doi-asserted-by":"publisher","first-page":"4510","DOI":"10.1021\/bi961889s","article-title":"Dynamics\nof cyanide binding to ferrous Scapharca inaequivalvis homodimeric hemoglobin","volume":"36","author":"Boffi","year":"1997","journal-title":"Biochemistry"},{"key":"2026102623013043300_cit31","doi-asserted-by":"publisher","first-page":"7351","DOI":"10.1021\/bi972864b","article-title":"Effect of iron-sulfur\ncluster environment in modulating the thermodynamic properties and\nbiological function of ferredoxin from Pyrococcus furiosus","volume":"37","author":"Brereton","year":"1998","journal-title":"Biochemistry"},{"key":"2026102623013043300_cit32","doi-asserted-by":"publisher","first-page":"6914","DOI":"10.1021\/ja101002f","article-title":"Identification\nof Protein-Bound Dinitrosyl Iron Complexes by Nuclear Resonance Vibrational\nSpectroscopy","volume":"132","author":"Tonzetich","year":"2010","journal-title":"J. Am. Chem. 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Global Consequences for the Disulfide\nOrientational Heterogeneity","volume":"38","author":"Wang","year":"1999","journal-title":"Biochemistry"},{"key":"2026102623013043300_cit41","doi-asserted-by":"publisher","first-page":"7215","DOI":"10.1039\/C5DT04760A","article-title":"Characterization\nof the [3Fe-4S] cluster from the D14C variant of Pyrococcus furiosus ferredoxin combined NRVS and DFT analyses","volume":"45","author":"Lauterbach","year":"2016","journal-title":"Dalton. 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