{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T15:01:54Z","timestamp":1785942114494,"version":"3.56.0"},"reference-count":40,"publisher":"American Chemical Society (ACS)","issue":"30","license":[{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-029"},{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-037"},{"start":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T00:00:00Z","timestamp":1784937600000},"content-version":"stm-asf","delay-in-days":0,"URL":"https:\/\/doi.org\/10.15223\/policy-045"}],"funder":[{"DOI":"10.13039\/501100006606","name":"Natural Science Foundation of Tianjin Municipality","doi-asserted-by":"publisher","award":["25JCJQJC00130"],"award-info":[{"award-number":["25JCJQJC00130"]}],"id":[{"id":"10.13039\/501100006606","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32572514"],"award-info":[{"award-number":["32572514"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100014103","name":"Key Technology Research and Development Program of Shandong Province","doi-asserted-by":"publisher","award":["2023CXGC010714"],"award-info":[{"award-number":["2023CXGC010714"]}],"id":[{"id":"10.13039\/100014103","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Tianjin Major Science and Technology Projects and Engineering Programs","award":["25ZXWCSY00240"],"award-info":[{"award-number":["25ZXWCSY00240"]}]},{"name":"Tianjin Major Science and Technology Projects and Engineering Programs","award":["25ZXZSSS00010"],"award-info":[{"award-number":["25ZXZSSS00010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,8,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Mycoprotein offers a sustainable approach to tackle global protein shortage, and Fusarium venenatum is an excellent chassis for mycoprotein production. However, low essential amino acid levels and poor functional properties limit its food application. In this study, we expressed chicken and duck ovalbumins in F. venenatum through multisite chromosomal integration. The engineered mycoprotein had 12.8% higher total protein and 39.5% more essential amino acids relative to the wild-type strain. Notably, the sulfur-containing amino acid (e.g., methionine and cysteine) content increased 3-fold. Its water\/oil holding, emulsifying, and gelling abilities were enhanced, with hardness 3.7\u20135.1 times that of the wild type. The modified strain reached 62.6% digestibility in 240 min, and its initial gastric digestion rate doubled. These results indicate that heterologous ovalbumin expression is a reliable method to upgrade the nutrition and functionality of mycoprotein, which will greatly expand the application of F. venenatum in the future food industry.<\/jats:p>","DOI":"10.1021\/acs.jafc.6c05785","type":"journal-article","created":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T05:11:54Z","timestamp":1785129114000},"page":"23662-23673","source":"Crossref","is-referenced-by-count":0,"title":["Expression of Ovalbumin\nin Edible\n                    <i>Fusarium venenatum<\/i>\n                    for Enhanced Nutritional and Functional Properties\nof Mycoprotein"],"prefix":"10.1021","volume":"74","author":[{"given":"Wenwen","family":"Pei","sequence":"first","affiliation":[{"name":"Tianjin University of Science and Technology , ,","place":["Tianjin, China, 300457"]},{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Chen","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]},{"name":"Key Laboratory of Engineering Biology for Low-carbon Manufacturing , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Zeng","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4392-4003","authenticated-orcid":true,"given":"Jiao","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3454-990X","authenticated-orcid":true,"given":"Xinjun","family":"Du","sequence":"additional","affiliation":[{"name":"Tianjin University of Science and Technology , ,","place":["Tianjin, China, 300457"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Men","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6592-3733","authenticated-orcid":true,"given":"Yueming","family":"Zhu","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]},{"name":"National Engineering Laboratory for Industrial Enzymes , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Demao","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0004-2721","authenticated-orcid":true,"given":"Qinhong","family":"Wang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8160-4564","authenticated-orcid":true,"given":"Yuanxia","family":"Sun","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]},{"name":"Key Laboratory of Engineering Biology for Low-carbon Manufacturing , ,","place":["Tianjin, China, 300308"]},{"name":"National Engineering Laboratory for Industrial Enzymes , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9463-8862","authenticated-orcid":true,"given":"Jiangang","family":"Yang","sequence":"additional","affiliation":[{"name":"Chinese Academy of Sciences , , ,","place":["Tianjin, China, 300308"]},{"name":"Key Laboratory of Engineering Biology for Low-carbon Manufacturing , ,","place":["Tianjin, China, 300308"]},{"name":"National Engineering Laboratory for Industrial Enzymes , ,","place":["Tianjin, China, 300308"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,7,25]]},"reference":[{"key":"2026080510104210900_cit1","doi-asserted-by":"publisher","first-page":"812","DOI":"10.1126\/science.1185383","article-title":"Food security: the challenge of feeding\n9 billion people","volume":"327","author":"Godfray","year":"2010","journal-title":"Science"},{"key":"2026080510104210900_cit2","doi-asserted-by":"publisher","first-page":"522","DOI":"10.1111\/ijfs.16791","article-title":"Mycoprotein as a meat\nsubstitute: production, functional properties,\nand current challenges-a review","volume":"59","author":"Khan","year":"2024","journal-title":"Int. J. Food\nSci."},{"key":"2026080510104210900_cit3","doi-asserted-by":"publisher","DOI":"10.1016\/j.fbio.2025.106882","article-title":"Pooling scientific\ninformation on\nthe nutritional, sensory, and technological properties of mycoprotein\nto support its role in creating a more sustainable food system","volume":"69","author":"Montebello","year":"2025","journal-title":"Food Biosci."},{"key":"2026080510104210900_cit4","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1016\/j.ymben.2025.09.009","article-title":"Edible fungus Fusarium venenatum:\nadvances, challenges, and engineering\nstrategies for future food production","volume":"93","author":"Tong","year":"2026","journal-title":"Metab.\nEng."},{"key":"2026080510104210900_cit5","doi-asserted-by":"publisher","DOI":"10.1016\/j.cofs.2024.101199","article-title":"What next\nfor mycoprotein?","volume":"58","author":"Linder","year":"2024","journal-title":"Curr. Opin. Food Sci."},{"key":"2026080510104210900_cit6","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1007\/s00253-002-0931-x","article-title":"Myco-protein\nfrom Fusarium venenatum: a well-established product\nfor human consumption","volume":"58","author":"Wiebe","year":"2002","journal-title":"Appl. Microbiol. Biotechnol."},{"issue":"6","key":"2026080510104210900_cit7","doi-asserted-by":"publisher","DOI":"10.1093\/cdn\/nzz021","article-title":"Mycoprotein: the\nfuture of nutritious nonmeat protein,\na symposium review","volume":"3","author":"Finnigan","year":"2019","journal-title":"Curr. Dev. Nutr."},{"key":"2026080510104210900_cit8","doi-asserted-by":"publisher","first-page":"604","DOI":"10.1021\/acs.jafc.3c08509","article-title":"Efficient\nmycoprotein production\nwith low CO2 emissions through metabolic engineering and\nfermentation optimization of Fusarium venenatum","volume":"72","author":"Tong","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"2026080510104210900_cit9","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1016\/j.synbio.2022.12.006","article-title":"Identification of neutral genome\nintegration sites with high expression\nand high integration efficiency in Fusarium venenatum TB01","volume":"8","author":"Tong","year":"2023","journal-title":"Synth. Syst. Biotechnol."},{"key":"2026080510104210900_cit10","doi-asserted-by":"publisher","DOI":"10.1186\/s40694-021-00121-8","article-title":"CRISPR\/Cas9 mediated\nediting of the Quorn fungus Fusarium venenatum A3\/5\nby transient expression of Cas9\nand sgRNAs targeting endogenous marker gene PKS12","volume":"8","author":"Wilson","year":"2021","journal-title":"Fungal Biol. Biotechnol."},{"key":"2026080510104210900_cit11","doi-asserted-by":"publisher","DOI":"10.1016\/j.biortech.2025.133005","article-title":"Cell wall\nengineering-guided strategy for high-efficiency biosynthesis of nutrientfortified Fusarium venenatum mycoprotein","volume":"436","author":"Wu","year":"2025","journal-title":"Bioresour.\nTechnol."},{"issue":"3","key":"2026080510104210900_cit12","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1016\/j.tibtech.2025.09.016","article-title":"Dual enhancement of mycoprotein nutrition\nand sustainability via CRISPR-mediated metabolic engineering of Fusarium venenatum","volume":"44","author":"Wu","year":"2026","journal-title":"Trends Biotechnol."},{"key":"2026080510104210900_cit13","doi-asserted-by":"publisher","first-page":"19462","DOI":"10.1021\/acs.jafc.4c06071","article-title":"Synchronous bioproduction of betanin\nand mycoprotein in the engineered edible fungus Fusarium venenatum","volume":"72","author":"Tong","year":"2024","journal-title":"J. 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Food\nChem."},{"key":"2026080510104210900_cit24","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1533\/9780857093639.335","article-title":"Mycoprotein:\norigins, production and properties","volume-title":"Handbook\nof Food Proteins","author":"Finnigan","year":"2011"},{"key":"2026080510104210900_cit25","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1016\/j.lwt.2018.01.058","article-title":"Comparison of functional\nproperties\nof edible insects and protein preparations thereof","volume":"91","author":"Zielin\u0301ska","year":"2018","journal-title":"LWT"},{"key":"2026080510104210900_cit26","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2020.126894","article-title":"Effect of protein and\noil volume concentrations on emulsifying properties of acorn protein\nisolate","volume":"324","author":"Aziz","year":"2020","journal-title":"Food Chem."},{"key":"2026080510104210900_cit27","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1016\/j.foodhyd.2014.07.003","article-title":"Understanding the differences in\ngelling properties between lupin protein isolate and soy protein isolate","volume":"43","author":"Berghout","year":"2015","journal-title":"Food Hydrocolloids"},{"key":"2026080510104210900_cit28","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1039\/C3FO60702J","article-title":"A standardised\nstatic in vitro digestion\nmethod suitable for food\u2500An international consensus","volume":"5","author":"Minekus","year":"2014","journal-title":"Food Funct."},{"key":"2026080510104210900_cit29","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1111\/j.1365-2621.2001.tb04614.x","article-title":"Improved method for determining food\nprotein degree of hydrolysis","volume":"66","author":"Nielsen","year":"2001","journal-title":"J. Food Sci."},{"key":"2026080510104210900_cit30","doi-asserted-by":"publisher","first-page":"S73","DOI":"10.1002\/jobm.200900018","article-title":"Cloning of\na novel ovalbumin gene from quail oviduct and its heterologous expression\nin Pichia pastoris","volume":"49","author":"Yang","year":"2009","journal-title":"J. Basic.\nMicrobiol."},{"issue":"15","key":"2026080510104210900_cit31","doi-asserted-by":"publisher","first-page":"8693","DOI":"10.1021\/acs.jafc.4c00827","article-title":"Constructing an antibiotic-free\nprotein expression system for ovalbumin\nbiosynthesis in probiotic Escherichia coli Nissle 1917","volume":"72","author":"Liu","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"2026080510104210900_cit32","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2022.112131","article-title":"Production\nof bovine beta-lactoglobulin and hen egg ovalbumin by Trichoderma reesei using precision fermentation technology and testing\nof their techno-functional properties","volume":"163","author":"Aro","year":"2023","journal-title":"Food\nRes. Int."},{"issue":"36","key":"2026080510104210900_cit33","doi-asserted-by":"publisher","first-page":"19985","DOI":"10.1021\/acs.jafc.4c05789","article-title":"Modification of the endoplasmic reticulum to enhance\novalbumin secretion in Saccharomyces cerevisiae","volume":"72","author":"Dong","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"2026080510104210900_cit34","doi-asserted-by":"publisher","first-page":"1917","DOI":"10.1023\/B:BILE.0000003987.60659.ea","article-title":"Immunological comparison\nof native and recombinant egg allergen,\novalbumin, expressed in Escherichia coli","volume":"25","author":"Rupa","year":"2003","journal-title":"Biotechnol. Lett."},{"issue":"2","key":"2026080510104210900_cit35","doi-asserted-by":"publisher","first-page":"346","DOI":"10.1111\/1751-7915.13027","article-title":"Protein profile\nin Aspergillus nidulans recombinant strains overproducing\nheterologous enzymes","volume":"11","author":"Zubieta","year":"2018","journal-title":"Microb. Biotechnol."},{"key":"2026080510104210900_cit36","doi-asserted-by":"publisher","DOI":"10.3390\/jof8020119","article-title":"Engineered fungus Thermothelomyces thermophilus producing plant storage proteins","volume":"8","author":"Balabanova","year":"2022","journal-title":"J. Fungi"},{"key":"2026080510104210900_cit37","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1016\/j.cbpc.2006.07.014","article-title":"Reactive\ncysteine in proteins: Protein folding, antioxidant defense, redox\nsignaling and more","volume":"146","author":"Netto","year":"2007","journal-title":"Comp. Biochem. Physiol.,\nPart C:Toxicol. Pharmacol."},{"issue":"2","key":"2026080510104210900_cit38","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1016\/j.febslet.2013.11.024","article-title":"Protein disulfide\nengineering","volume":"588","author":"Dombkowski","year":"2014","journal-title":"FEBS Lett."},{"key":"2026080510104210900_cit39","doi-asserted-by":"publisher","DOI":"10.1016\/j.focha.2023.100299","article-title":"Effects of\nThree Extraction Methods on the Structural and Functional Properties\nof Insoluble Dietary Fibers from Mycoprotein","volume":"2","author":"Zhang","year":"2023","journal-title":"Food Chem. Adv."},{"key":"2026080510104210900_cit40","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2025.117498","article-title":"Tuning Fusarium venenatum mycelium structure through\ngenetic modification for customized texture and digestibility of chicken\nbreast analogues","volume":"221","author":"Wu","year":"2025","journal-title":"Food Res. Int."}],"container-title":["Journal of Agricultural\nand Food Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acs.jafc.6c05785","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/30\/23662\/66254514\/acs.jafc.6c05785.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/pubs.acs.org\/jafcau\/article-pdf\/74\/30\/23662\/66254514\/acs.jafc.6c05785.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T14:24:12Z","timestamp":1785939852000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/jafcau\/article\/74\/30\/23662\/5232295\/Expression-of-Ovalbumin-in-Edible-Fusarium"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,7,25]]},"references-count":40,"journal-issue":{"issue":"30","published-online":{"date-parts":[[2026,7,25]]},"published-print":{"date-parts":[[2026,8,5]]}},"URL":"https:\/\/doi.org\/10.1021\/acs.jafc.6c05785","relation":{},"ISSN":["0021-8561","1520-5118"],"issn-type":[{"value":"0021-8561","type":"print"},{"value":"1520-5118","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,7,25]]}}}