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This study provides a combined proteomic and lipidomic analysis of serum and urine from naturally aging domestic cats aged 3.8\u201316 years, grouped as adult, old, and senior, to identify age-related molecular changes across biofluids. Label-free quantitative proteomics identified 901 urinary and 238 serum proteins, with 75 urinary proteins significantly altered with age that are linked to kidney disease, hypertension, neurodegeneration, and metabolic disorders. In contrast, only six serum proteins differed significantly between adult and old\/senior cats, including decreased Apolipoprotein A-I (APOA1) in seniors, a protein linked with cognitive function in aging. Untargeted lipidomics revealed increases in specific serum triacylglycerols, phosphatidylcholines (PCs), and sphingomyelins, while urinary lipid profiles showed limited age-related changes, with some PCs decreasing, and diacylglycerols increasing with age. These results demonstrate distinct systemic and renal molecular remodeling during feline aging and highlight the utility of integrated omics analyses of biological fluids for identifying molecular alterations relevant to both feline and human aging.<\/jats:p>","DOI":"10.1021\/acs.jproteome.6c00247","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T17:02:15Z","timestamp":1786467735000},"page":"4719-4733","source":"Crossref","is-referenced-by-count":0,"title":["Urine and\nSerum Proteome and Lipidome Analysis of\nNaturally Aging Feline Species"],"prefix":"10.1021","volume":"25","author":[{"given":"Punyatoya","family":"Panda","sequence":"first","affiliation":[{"name":"Purdue University , , , ,","place":["West Lafayette, Indiana, United States, 47907"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3680-1291","authenticated-orcid":true,"given":"Rodrigo","family":"Mohallem","sequence":"additional","affiliation":[{"name":"Purdue University , , , ,","place":["West Lafayette, Indiana, United States, 47907"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2380-0253","authenticated-orcid":true,"given":"Rita","family":"de Kassia Rodrigues Bezerra Filgueira","sequence":"additional","affiliation":[{"name":"Purdue University , , , ,","place":["West Lafayette, Indiana, United States, 47907"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niwako","family":"Ogata","sequence":"additional","affiliation":[{"name":"Purdue University , , , ,","place":["West Lafayette, Indiana, United States, 47907"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4543-1536","authenticated-orcid":true,"given":"Uma K.","family":"Aryal","sequence":"additional","affiliation":[{"name":"Purdue University , , , ,","place":["West Lafayette, Indiana, United States, 47907"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"316","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"2026090409123628300_cit1","doi-asserted-by":"publisher","first-page":"683459","DOI":"10.3389\/fcell.2021.683459","article-title":"The Ageing\nBrain: Molecular and Cellular Basis of Neurodegeneration","volume":"9","author":"Azam","year":"2021","journal-title":"Front. 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