{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T06:03:57Z","timestamp":1768975437176,"version":"3.49.0"},"reference-count":80,"publisher":"American Chemical Society (ACS)","issue":"5","license":[{"start":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T00:00:00Z","timestamp":1768867200000},"content-version":"vor","delay-in-days":278,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["10.54499\/CEECIND\/04050\/2017\/CP1459\/CT0023"],"award-info":[{"award-number":["10.54499\/CEECIND\/04050\/2017\/CP1459\/CT0023"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["2020.09018.BD"],"award-info":[{"award-number":["2020.09018.BD"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["2021.01571.CEECIND"],"award-info":[{"award-number":["2021.01571.CEECIND"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["CEECIND\/00464\/2017"],"award-info":[{"award-number":["CEECIND\/00464\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["CEECIND\/02387\/2018"],"award-info":[{"award-number":["CEECIND\/02387\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["CENTRO-01-0145-FEDER-031289"],"award-info":[{"award-number":["CENTRO-01-0145-FEDER-031289"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0006\/2020"],"award-info":[{"award-number":["LA\/P\/0006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0008\/202"],"award-info":[{"award-number":["LA\/P\/0008\/202"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["LA\/P\/0094\/2020"],"award-info":[{"award-number":["LA\/P\/0094\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/140229\/2018"],"award-info":[{"award-number":["SFRH\/BD\/140229\/2018"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/50006\/2020"],"award-info":[{"award-number":["UIDB\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/50011\/2020"],"award-info":[{"award-number":["UIDB\/50011\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDB\/50017\/2020"],"award-info":[{"award-number":["UIDB\/50017\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/50006\/2020"],"award-info":[{"award-number":["UIDP\/50006\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/50011\/2020"],"award-info":[{"award-number":["UIDP\/50011\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda??o para a Ci?ncia e a Tecnologia","doi-asserted-by":"publisher","award":["UIDP\/50017\/2020"],"award-info":[{"award-number":["UIDP\/50017\/2020"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["ACS Biomater. Sci. Eng."],"published-print":{"date-parts":[[2025,5,12]]},"DOI":"10.1021\/acsbiomaterials.4c02148","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T01:04:43Z","timestamp":1744851883000},"page":"3043-3057","source":"Crossref","is-referenced-by-count":4,"title":["Biofabrication of HepG2 Cells-Laden 3D Structures Using Nanocellulose-Reinforced Gelatin-Based Hydrogel Bioinks: Materials Characterization, Cell Viability Assessment, and Metabolomic Analysis"],"prefix":"10.1021","volume":"11","author":[{"given":"Nicole S.","family":"Lameirinhas","sequence":"first","affiliation":[{"name":"CICECO\u2500Aveiro Institute of Materials, Department of Chemistry","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]}]},{"given":"Maria C.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"CICECO\u2500Aveiro Institute of Materials, Department of Chemistry","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]}]},{"given":"Jo\u00e3o P. F.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"CICECO\u2500Aveiro Institute of Materials, Department of Chemistry","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7252-4933","authenticated-orcid":true,"given":"Bruno F. A.","family":"Valente","sequence":"additional","affiliation":[{"name":"CICECO\u2500Aveiro Institute of Materials, Department of Chemistry","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8846-439X","authenticated-orcid":true,"given":"Jorge L.","family":"Lu\u00eds","sequence":"additional","affiliation":[{"name":"EMaRT Group\u2500Emerging: Materials, Research, Technology, School of Design, Management and Production Technologies Northern Aveiro","place":["Oliveira de Azem\u00e9is, Portugal"]},{"name":"University of Aveiro","place":["Oliveira de Azem\u00e9is, Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4289-9256","authenticated-orcid":true,"given":"Iola F.","family":"Duarte","sequence":"additional","affiliation":[{"name":"CICECO\u2500Aveiro Institute of Materials, Department of Chemistry","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]},{"name":"LAQV-REQUIMTE","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5600-2396","authenticated-orcid":true,"given":"Ricardo J. B.","family":"Pinto","sequence":"additional","affiliation":[{"name":"CICECO\u2500Aveiro Institute of Materials, Department of Chemistry","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4673-0696","authenticated-orcid":true,"given":"Helena","family":"Oliveira","sequence":"additional","affiliation":[{"name":"CESAM\u2500Centre for Environmental and Marine Studies, Department of Biology","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1564-5728","authenticated-orcid":true,"given":"Jos\u00e9 M.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"EMaRT Group\u2500Emerging: Materials, Research, Technology, School of Design, Management and Production Technologies Northern Aveiro","place":["Oliveira de Azem\u00e9is, Portugal"]},{"name":"University of Aveiro","place":["Oliveira de Azem\u00e9is, Portugal"]}]},{"given":"Armando J. D.","family":"Silvestre","sequence":"additional","affiliation":[{"name":"CICECO\u2500Aveiro Institute of Materials, Department of Chemistry","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9212-2704","authenticated-orcid":true,"given":"Carla","family":"Vilela","sequence":"additional","affiliation":[{"name":"CICECO\u2500Aveiro Institute of Materials, Department of Chemistry","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6320-4663","authenticated-orcid":true,"given":"Carmen S. R.","family":"Freire","sequence":"additional","affiliation":[{"name":"CICECO\u2500Aveiro Institute of Materials, Department of Chemistry","place":["Aveiro, Portugal"]},{"name":"University of Aveiro","place":["Aveiro, Portugal"]}]}],"member":"316","published-online":{"date-parts":[[2025,4,17]]},"reference":[{"key":"ref1\/cit1","doi-asserted-by":"publisher","DOI":"10.3390\/nano11020481"},{"key":"ref2\/cit2","doi-asserted-by":"publisher","DOI":"10.5114\/aoms.2016.63743"},{"key":"ref3\/cit3","doi-asserted-by":"publisher","DOI":"10.1016\/j.apmt.2019.100479"},{"key":"ref4\/cit4","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2958"},{"key":"ref5\/cit5","doi-asserted-by":"publisher","DOI":"10.3389\/fmech.2020.589171"},{"key":"ref6\/cit6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2022.12.227"},{"key":"ref7\/cit7","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00566-8"},{"key":"ref8\/cit8","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.3c07077"},{"key":"ref9\/cit9","doi-asserted-by":"publisher","DOI":"10.1038\/s41698-020-0121-2"},{"key":"ref10\/cit10","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ac925a"},{"key":"ref11\/cit11","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c00920"},{"key":"ref12\/cit12","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c06658"},{"key":"ref13\/cit13","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.761861"},{"key":"ref14\/cit14","doi-asserted-by":"publisher","DOI":"10.3390\/cells8101295"},{"key":"ref15\/cit15","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00878"},{"key":"ref16\/cit16","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2020.120416"},{"key":"ref17\/cit17","doi-asserted-by":"publisher","DOI":"10.1016\/j.bprint.2020.e00080"},{"key":"ref18\/cit18","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/abec2c"},{"key":"ref19\/cit19","doi-asserted-by":"publisher","DOI":"10.1016\/j.bprint.2022.e00212"},{"key":"ref20\/cit20","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23126564"},{"key":"ref21\/cit21","doi-asserted-by":"publisher","DOI":"10.1002\/macp.201900126"},{"key":"ref22\/cit22","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2017.04.019"},{"key":"ref23\/cit23","doi-asserted-by":"publisher","DOI":"10.3390\/nano12132190"},{"key":"ref24\/cit24","doi-asserted-by":"publisher","DOI":"10.3390\/polym9090401"},{"key":"ref25\/cit25","doi-asserted-by":"publisher","DOI":"10.1016\/j.matpr.2020.12.922"},{"key":"ref26\/cit26","doi-asserted-by":"publisher","DOI":"10.1007\/s10856-016-5763-9"},{"key":"ref27\/cit27","doi-asserted-by":"publisher","DOI":"10.1039\/C7BM00765E"},{"key":"ref28\/cit28","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-016-1638-y"},{"key":"ref29\/cit29","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering5030055"},{"key":"ref30\/cit30","unstructured":"PhotoGel-INK 95% DS, Gelatin Methacrylate\nBioink\u2500CELLINK. https:\/\/www.cellink.com\/product\/photogel-ink-95-dom\/ (accessed\n03 23, 2024)."},{"key":"ref31\/cit31","unstructured":"GelMA PhotoInk\n                      TM\n                      5mL\u2500CELLINK. https:\/\/www.cellink.com\/product\/gelma-photoink-5ml\/ (accessed 03 23, 2024)."},{"key":"ref32\/cit32","unstructured":"GelMA Bioink LAP\n0.25%\u2500CELLINK. https:\/\/www.cellink.com\/product\/gelma-bioink-lap-0-25\/ (accessed 03 23, 2024)."},{"key":"ref33\/cit33","unstructured":"GelXG\u2500CELLINK. https:\/\/www.cellink.com\/product\/gelxg\/ (accessed 03 23, 2024)."},{"key":"ref34\/cit34","doi-asserted-by":"publisher","DOI":"10.1016\/j.slast.2023.02.003"},{"key":"ref35\/cit35","doi-asserted-by":"publisher","DOI":"10.1186\/s40779-022-00429-5"},{"key":"ref36\/cit36","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering7020040"},{"key":"ref37\/cit37","doi-asserted-by":"publisher","DOI":"10.3390\/polym11050898"},{"key":"ref38\/cit38","doi-asserted-by":"publisher","DOI":"10.1515\/9783110574432-001"},{"key":"ref39\/cit39","doi-asserted-by":"publisher","DOI":"10.1016\/j.mattod.2018.02.001"},{"key":"ref40\/cit40","doi-asserted-by":"publisher","DOI":"10.1088\/1758-5090\/ac8768"},{"key":"ref41\/cit41","doi-asserted-by":"publisher","DOI":"10.15376\/biores.12.2.2941-2954"},{"key":"ref42\/cit42","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.2c16126"},{"key":"ref43\/cit43","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/3863428"},{"key":"ref44\/cit44","doi-asserted-by":"publisher","DOI":"10.1021\/acsami.8b21268"},{"key":"ref45\/cit45","doi-asserted-by":"publisher","DOI":"10.2174\/15672018113106660059"},{"key":"ref46\/cit46","doi-asserted-by":"publisher","DOI":"10.3390\/catal9121035"},{"key":"ref47\/cit47","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodhyd.2021.106709"},{"key":"ref48\/cit48","doi-asserted-by":"publisher","DOI":"10.1016\/B978-075064885-1\/50005-3"},{"key":"ref49\/cit49","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biomac.0c01476"},{"key":"ref50\/cit50","doi-asserted-by":"publisher","DOI":"10.1038\/srep29977"},{"key":"ref51\/cit51","doi-asserted-by":"publisher","DOI":"10.1002\/pen.25821"},{"key":"ref52\/cit52","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-20006-y"},{"key":"ref53\/cit53","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23010539"},{"key":"ref54\/cit54","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpharm.2022.121506"},{"key":"ref55\/cit55","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmst.2022.07.011"},{"key":"ref56\/cit56","doi-asserted-by":"publisher","DOI":"10.3390\/ma16020572"},{"key":"ref57\/cit57","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2021.766399"},{"key":"ref58\/cit58","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnnfm.2016.11.001"},{"key":"ref59\/cit59","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2020.129782"},{"key":"ref60\/cit60","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.0c00084"},{"key":"ref61\/cit61","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.640388"},{"key":"ref62\/cit62","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2016.02.016"},{"key":"ref63\/cit63","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X1000501202"},{"key":"ref64\/cit64","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-5629(02)00489-1"},{"key":"ref65\/cit65","doi-asserted-by":"publisher","DOI":"10.1016\/j.bprint.2022.e00201"},{"key":"ref66\/cit66","doi-asserted-by":"publisher","DOI":"10.1089\/ten.teb.2009.0639"},{"key":"ref67\/cit67","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2018.03.039"},{"key":"ref68\/cit68","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.194"},{"key":"ref69\/cit69","unstructured":"International Organization\nfor Standardization\n                      ISO 10993-5:2009\nBiological Evaluation of Medical Devices\u2500Part 5: Tests for\nin Vitro Cytotoxicity\n                      ; International Organization\nfor Standardization: Geneva, Switzerland, 2017."},{"key":"ref70\/cit70","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.06.039"},{"key":"ref71\/cit71","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.9b00275"},{"key":"ref72\/cit72","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.a.35717"},{"key":"ref73\/cit73","doi-asserted-by":"publisher","DOI":"10.1039\/C7PY00826K"},{"key":"ref74\/cit74","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2023.1093101"},{"key":"ref75\/cit75","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2015.12.001"},{"key":"ref76\/cit76","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbabio.2012.05.010"},{"key":"ref77\/cit77","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2018.00637"},{"key":"ref78\/cit78","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2017.98"},{"key":"ref79\/cit79","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108340"},{"key":"ref80\/cit80","doi-asserted-by":"publisher","DOI":"10.1002\/mc.20539"}],"container-title":["ACS Biomaterials Science & Engineering"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsbiomaterials.4c02148","content-type":"application\/pdf","content-version":"vor","intended-application":"unspecified"},{"URL":"https:\/\/pubs.acs.org\/doi\/pdf\/10.1021\/acsbiomaterials.4c02148","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,20]],"date-time":"2026-01-20T17:03:23Z","timestamp":1768928603000},"score":1,"resource":{"primary":{"URL":"https:\/\/pubs.acs.org\/doi\/10.1021\/acsbiomaterials.4c02148"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,17]]},"references-count":80,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2025,5,12]]}},"alternative-id":["10.1021\/acsbiomaterials.4c02148"],"URL":"https:\/\/doi.org\/10.1021\/acsbiomaterials.4c02148","relation":{},"ISSN":["2373-9878","2373-9878"],"issn-type":[{"value":"2373-9878","type":"print"},{"value":"2373-9878","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,17]]}}}