{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:27:19Z","timestamp":1760887639755,"version":"build-2065373602"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":5422,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[1993,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new aqueous two\u2010phase system based on crude hydroxypropyl starch (HPS) and poly(ethylene glycol) (PEG) is described. Unlike other polymer\u2010polymer aqueous two\u2010phase systems, the HPS used is commercially available. Previous works have characterized almost exclusively purified carbohydrates or their derivatives, having neglected the large amount of gums and carbohydrates already used in the paper, food, or textile industries. Two\u2010phase aqueous polymer systems based on such commercial polymers may have as a drawback their degree of purity, which may affect the separation procedure. However, their lower cost should encourage studies on their use in aqueous two\u2010phase polymer extraction. The influence of temperature, pH, and ionic strength on the partition of several different molecular weight and isoelectric point proteins and whole cells was studied. The results obtained were compared with corresponding dextran\u2010PEG systems, Aquaphase PPT\u2010PEG systems, crude dextran\u2010PEG systems, maltodextrin\u2010PEG systems, and Klucel L\u2010pluronic systems. Protein partition coefficient results exhibited higher values than those in corresponding systems. It is shown that this system, based on a crude hydroxypropyl starch, is highly attractive as an initial purification step.<\/jats:p>","DOI":"10.1021\/bp00024a010","type":"journal-article","created":{"date-parts":[[2005,3,7]],"date-time":"2005-03-07T21:49:12Z","timestamp":1110232152000},"page":"635-639","source":"Crossref","is-referenced-by-count":19,"title":["Evaluation of Crude Hydroxypropyl Starch As a Bioseparation Aqueous\u2010Phase\u2010Forming Polymer"],"prefix":"10.1002","volume":"9","author":[{"given":"Armando","family":"Ven\u01cencio","sequence":"first","affiliation":[]},{"given":"Jos\u00e9 A.","family":"Teixeira","sequence":"additional","affiliation":[]},{"given":"Manuel","family":"Mota","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"191","article-title":"On protein partition in two\u2010phase aqueous polymer systems","volume":"1","author":"Abbot N. 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