{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:55:11Z","timestamp":1760061311099,"version":"build-2065373602"},"reference-count":16,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":2804,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2001,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This paper describes the bioconversion of 2\u2010methyl\u20101,3\u2010propanediol to (<jats:italic>R<\/jats:italic>)\u2010\u03b2\u2010hydoxyisobutyric acid (HIBA) by <jats:italic>Acetobacter<\/jats:italic> ALEI in a hollow fiber membrane bioreaction system arrangement that allows the integration of three liquid phases: the aqueous bioconversion phase, the organic phase consisting of a solution of trioctyl phosphine oxide (TOPO) in isooctane, and the third phase consisting of a basic stripping solution that allows reextraction of HIBA from the organic phase. A comparison of HIBA mass transfer experiments was carried out in the membrane reactor with two and three phases for different pH and TOPO concentrations. The use of the three\u2010phase arrangement allows the extraction of high quantities of HIBA from the aqueous medium (higher than 85%) independently of the pH, whereas in the two\u2010phase system the percentage of HIBA extracted from the aqueous medium was lower, 42% in the best case, and strongly influenced by the pH. The percentage of the extractive agent TOPO in the organic phase influenced on the mass transfer rate in both bi\u2010 and triphasic arrangements. By simply integrating the re\u2010extraction phase in the system it was possible to increase the extraction yield by 2\u2010fold, reduce the amount of TOPO by 4\u2010fold, and operate at the more favorable pH 4. A bioconversion experiment was done in these conditions (pH = 4, TOPO = 5%) to confirm the advantages of including the third stripping solution. Fed\u2010batch operation of the triphasic membrane reactor was maintained for more than 20 h, reaching an HIBA concentration in the stripping solution of 29 g L<jats:sup>\u2212<\/jats:sup><jats:sup>1<\/jats:sup>.<\/jats:p>","DOI":"10.1021\/bp010015g","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T00:58:44Z","timestamp":1027645124000},"page":"468-473","source":"Crossref","is-referenced-by-count":12,"title":["A Multiphasic Hollow Fiber Reactor for the Whole\u2010Cell Bioconversion of 2\u2010Methyl\u20101,3\u2010propanediol to (<i>R<\/i>)\u2010\u03b2\u2010Hydroxyisobutyric Acid"],"prefix":"10.1002","volume":"17","author":[{"given":"Rosa","family":"Le\u00f3n","sequence":"first","affiliation":[]},{"given":"Duarte M. F.","family":"Prazeres","sequence":"additional","affiliation":[]},{"given":"Pedro","family":"Fernandes","sequence":"additional","affiliation":[]},{"given":"Francesco","family":"Molinari","sequence":"additional","affiliation":[]},{"given":"Joaquim M. S.","family":"Cabral","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_4_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260280217"},{"key":"e_1_2_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(98)00078-7"},{"key":"e_1_2_4_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/aic.690370309"},{"key":"e_1_2_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0921-0423(98)80101-9"},{"key":"e_1_2_4_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0141-0229(94)00086-7"},{"key":"e_1_2_4_7_2","first-page":"181","volume-title":"Recent Advances in Marine Biotechnology","author":"Prazeres D. M. F.","year":"1998"},{"key":"e_1_2_4_8_2","first-page":"27","volume-title":"Extractive Bioconversions","author":"L\u00f3pez J. L.","year":"1991"},{"key":"e_1_2_4_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3548-5_41"},{"key":"e_1_2_4_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/jo00133a033"},{"key":"e_1_2_4_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4660(200007)75:7<617::AID-JCTB110>3.0.CO;2-1"},{"key":"e_1_2_4_12_2","doi-asserted-by":"crossref","unstructured":"Le\u00f3n R.; Molinari F.;Prazeres D. M. F.;Cabral J. M. S.Microbial Stereoselective Oxidation of 2\u2010methyl\u20101 3\u2010propanediol to (R)\u2010\u03b2\u2010hydroxyisobutyric Acid in Aqueous\/Organic Biphasic System.Biocatal. Biotransform.2001 submitted for publication.","DOI":"10.1080\/10242420290020723"},{"key":"e_1_2_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(96)00205-0"},{"key":"e_1_2_4_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/btpr.5420020110"},{"key":"e_1_2_4_15_2","first-page":"323","volume-title":"Immobilised Living Cell Systems: Modelling and Experimental Methods","author":"Heath C. 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