{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T09:54:19Z","timestamp":1761558859330,"version":"build-2065373602"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,9,5]],"date-time":"2008-09-05T00:00:00Z","timestamp":1220572800000},"content-version":"vor","delay-in-days":2439,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Biotechnology Progress"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Enzyme performance is often impaired in supercritical carbon dioxide. We were able to enhance enzyme activity in this medium via changes in acid\u2010base conditions by using ion\u2010exchange materials (solid H\n                    <jats:sup>+<\/jats:sup>\n                    \/Na\n                    <jats:sup>+<\/jats:sup>\n                    buffer pairs and a zeolite), which were selected on the basis of the response of an organosoluble acid\u2010base indicator. The concentration of ion\u2010exchange materials had an important effect on the catalytic activity of subtilisin Carlsberg cross\u2010linked enzyme crystals (CLECs), and this was related to the protonation and hydration states of the enzyme. The buffer Na\n                    <jats:sub>2<\/jats:sub>\n                    CO\n                    <jats:sub>3<\/jats:sub>\n                    \/NaHCO\n                    <jats:sub>3<\/jats:sub>\n                    gave the highest enhancement in enzyme activity (by a factor of 54), probably as a result of its high basicity and capacity to counteract the deleterious effect of carbonic acid to a greater extent than the other materials tested.\n                  <\/jats:p>","DOI":"10.1021\/bp025602w","type":"journal-article","created":{"date-parts":[[2002,12,6]],"date-time":"2002-12-06T00:41:53Z","timestamp":1039135313000},"page":"1451-1454","source":"Crossref","is-referenced-by-count":21,"title":["Enhancement of Enzyme Activity in Supercritical Carbon Dioxide via Changes in Acid\u2010Base Conditions"],"prefix":"10.1002","volume":"18","author":[{"given":"Neil","family":"Harper","sequence":"first","affiliation":[]},{"given":"Susana","family":"Barreiros","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2008,9,5]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/cr970040u"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.260390304"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.3109\/07388559509150531"},{"key":"e_1_2_5_5_2","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1002\/(SICI)1097-0290(19960220)49:4<399::AID-BIT6>3.0.CO;2-K","article-title":"Solvent effects on the catalytic activity of subtilisin suspended in compressed gases","volume":"49","author":"Borges de Carvalho I.","year":"1996","journal-title":"Biotechnol. Bioeng."},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/bp000148m"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(99)00055-1"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-3765(20000602)6:11<1923::AID-CHEM1923>3.0.CO;2-T"},{"key":"e_1_2_5_9_2","doi-asserted-by":"crossref","unstructured":"Partridge J.;Halling P. J.;Moore B. D.Solid\u2010state proton\/sodium buffers: \u201cChemical pH stats\u201d for biocatalysts in organic solvents.J. Chem. Soc. Perkin Trans. 22000 465\u2013471.","DOI":"10.1039\/a908673k"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10138"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja961383x"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-112-4:565"},{"key":"e_1_2_5_13_2","unstructured":"Although none of the buffers act as such in sc\u2010CO2 we keep the term buffer for convenience."},{"key":"e_1_2_5_14_2","unstructured":"Because of the lowawat which transesterification was carried out it was not possible to detect Ac\u2010Phe as a result of hydrolysis of the substrate ester. Thus the buffers used only had to counteract the effect of carbonic acid."},{"key":"e_1_2_5_15_2","unstructured":"Both Na2CO3and NaHCO3have an affinity for water."},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.3.1100"}],"container-title":["Biotechnology Progress"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1021%2Fbp025602w","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/pdf\/10.1021\/bp025602w","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T09:43:29Z","timestamp":1761558209000},"score":1,"resource":{"primary":{"URL":"https:\/\/aiche.onlinelibrary.wiley.com\/doi\/10.1021\/bp025602w"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1]]},"references-count":15,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1021\/bp025602w"],"URL":"https:\/\/doi.org\/10.1021\/bp025602w","archive":["Portico"],"relation":{},"ISSN":["8756-7938","1520-6033"],"issn-type":[{"type":"print","value":"8756-7938"},{"type":"electronic","value":"1520-6033"}],"subject":[],"published":{"date-parts":[[2002,1]]}}}