{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T13:29:54Z","timestamp":1762954194511},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Inher Metab Disea"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We investigated the quantitative expression of the human glucose\u20106\u2010phosphate translocase gene (<jats:italic>G6PT1<\/jats:italic>) and its splicing variants in human tissues. The <jats:italic>G6PT1<\/jats:italic> gene was strongly expressed in liver, kidney and haematopoietic progenitor cells, which might explain major clinical symptoms such as hepatomegaly, nephromegaly and neutropenia in glycogen storage diseases type Ib. Reverse transcriptase\u2010mediated PCR amplification of <jats:italic>G6PT1<\/jats:italic> cDNA revealed several splicing variants in tissue\u2010specific manners. The brain\u2010specific isoform, which has an additional 22 amino acids between exons 6 and 8, was also identified in heart and skeletal muscle. A new splicing variant, although less prominent in quantity and lacking polypeptide loops corresponding to exons 2 and 3, may have a distinct substrate affinity or specificity in leukocytes and haematopoietic progenitors. In conclusion, the <jats:italic>G6PT1<\/jats:italic> gene was expressed in various tissues, and alternative splicing variants exist in tissue\u2010specific manners.<\/jats:p>","DOI":"10.1023\/a:1005677912539","type":"journal-article","created":{"date-parts":[[2002,12,21]],"date-time":"2002-12-21T23:07:17Z","timestamp":1040512037000},"page":"583-592","source":"Crossref","is-referenced-by-count":25,"title":["Quantitative analysis of glucose\u20106\u2010phosphate translocase gene expression in various human tissues and haematopoietic progenitor cells"],"prefix":"10.1002","volume":"23","author":[{"given":"K.","family":"Ihara","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Graduate School of Medical Sciences Kyushu University  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Nomura","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Graduate School of Medical Sciences Kyushu University  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Hikino","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Graduate School of Medical Sciences Kyushu University  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Takada","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Graduate School of Medical Sciences Kyushu University  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Hara","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Graduate School of Medical Sciences Kyushu University  Fukuoka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2000,9]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(80)80418-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(91)90146-O"},{"key":"e_1_2_1_4_1","first-page":"935","volume-title":"The Metabolic and Molecular Bases of Inherited Disease","author":"Chen Y","year":"1995"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(97)01463-4"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(98)00614-3"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.10.995"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02072085"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.10.986"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004390050856"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1159\/000015167"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.8985"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(05)80403-2"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)43502-8"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.48.31656"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)01129-6"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1203\/00006450-199401000-00017"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(98)00878-3"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.11.4789.bloodjournal87114789"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(78)91371-2"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(05)80290-2"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1086\/302068"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejhg.5200366"}],"container-title":["Journal of Inherited Metabolic Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1023%2FA%3A1005677912539","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1023\/A%3A1005677912539","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T22:44:13Z","timestamp":1694817853000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1023\/A%3A1005677912539"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,9]]}},"alternative-id":["10.1023\/A:1005677912539"],"URL":"https:\/\/doi.org\/10.1023\/a:1005677912539","archive":["Portico"],"relation":{},"ISSN":["0141-8955","1573-2665"],"issn-type":[{"value":"0141-8955","type":"print"},{"value":"1573-2665","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,9]]}}}