{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T12:37:01Z","timestamp":1774355821699,"version":"3.50.1"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"1-3","license":[{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"},{"start":{"date-parts":[[1999,7,1]],"date-time":"1999-07-01T00:00:00Z","timestamp":930787200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springernature.com\/gp\/researchers\/text-and-data-mining"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytotechnology"],"published-print":{"date-parts":[[1999,7]]},"DOI":"10.1023\/a:1008021317639","type":"journal-article","created":{"date-parts":[[2002,12,22]],"date-time":"2002-12-22T07:45:23Z","timestamp":1040543123000},"page":"191-201","source":"Crossref","is-referenced-by-count":27,"title":["The new medium MDSS2N, free of any animal protein supports cell growth and production of various viruses"],"prefix":"10.1007","volume":"30","author":[{"given":"O.-W.","family":"Merten","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Kallel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.-C.","family":"Manuguerra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Tardy-Panit","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Crainic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F.","family":"Delpeyroux","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Van der Werf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Perrin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","reference":[{"key":"192980_CR1","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/978-94-011-5404-8_27","volume-title":"Animal Cell Technology: From Vaccines to Genetic Medicine","author":"R Brands","year":"1997","unstructured":"Brands R, van Scharrenburg GJM, and Palache AM (1997) Production of influenza virus in cell cultures for vaccine preparation. In: Carrondo MJT, Griffiths B and Moreira J (eds) Animal Cell Technology: From Vaccines to Genetic Medicine, (pp. 165-167) Kluwer Academic Publishers, Dordrecht, The Netherlands."},{"key":"192980_CR2","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1006\/cbir.1993.1152","volume":"17","author":"J Cinatl Jr","year":"1993","unstructured":"Cinatl J Jr, Cinatl J, Rabenau H, Rapp J, Kornhuber B and Doerr H-W (1993) Protein-free culture of Vero cells: A substrate for replication of human pathogenic viruses. Cell Biol Int 17, 885-895.","journal-title":"Cell Biol Int"},{"key":"192980_CR3","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1016\/S1045-1056(05)80063-6","volume":"20","author":"Committee for Proprietary Medicinal Products: Ad Hoc Working Party on Biotechnology\/PharmacyWorking Party on Safety Medicines","year":"1992","unstructured":"Committee for Proprietary Medicinal Products: Ad Hoc Working Party on Biotechnology\/Pharmacy and Working Party on Safety Medicines (1992) EEC Regulatory Document, Note for Guidance. Guidelines for minimizing the risk of transmitting agents causing spongiform encephalopathy via medicinal products. Biologicals 20, 155-158.","journal-title":"Biologicals"},{"key":"192980_CR4","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1007\/BF00365077","volume":"1","author":"V J\u00e4ger","year":"1988","unstructured":"J\u00e4ger V, Lehmann J and Friedl P (1988) Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors. Cytotechnol 1, 319-329.","journal-title":"Cytotechnol"},{"key":"192980_CR5","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1007\/BF00761775","volume":"16","author":"DC-H Jan","year":"1994","unstructured":"Jan DC-H, Jones SJ, Emery AN and Al-Rubeai M (1994) Peptone, a low-cost growth-promoting nutrient for intensive animal cell culture. Cytotechnol 16, 17-26.","journal-title":"Cytotechnol"},{"key":"192980_CR6","doi-asserted-by":"publisher","first-page":"745","DOI":"10.1002\/bit.260170510","volume":"17","author":"L Keay","year":"1975","unstructured":"Keay L (1975) Autoclavable low cost serum-free cell culture media. The growth of L-cells and BHK cells on peptones. Biotechnol Bioeng 17, 745-764.","journal-title":"Biotechnol Bioeng"},{"key":"192980_CR7","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1002\/bit.260190310","volume":"19","author":"L Keay","year":"1977","unstructured":"Keay L (1977) The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Biotechnol Bioeng 19, 399-411.","journal-title":"Biotechnol Bioeng"},{"key":"192980_CR8","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/S0091-679X(08)62019-1","volume":"20","author":"L Keay","year":"1978","unstructured":"Keay L (1978) The cultivation of animal cells and production of viruses in serum systems. Meth Cell Biol 20, 169-209.","journal-title":"Meth Cell Biol"},{"key":"192980_CR9","doi-asserted-by":"publisher","first-page":"243","DOI":"10.1023\/A:1007916930200","volume":"24","author":"J Keenan","year":"1997","unstructured":"Keenan J, Dooley M, Pearson D and Clynes M (1997) Recombinant human albumin in cell culture: Evaluation of growth-promoting potential for NRK and SCC-9 cells in vitro. Cytotechnol 24, 243-252.","journal-title":"Cytotechnol"},{"key":"192980_CR10","first-page":"555","volume-title":"New Developments and New Applications in Animal Cell Technology","author":"N Kessler","year":"1998","unstructured":"Kessler N, Thomas G, Gerents L and Aymard M (1998) Serum-free MDCK-cells: an alternative for influenza vaccine virus production. In: Merten OW, Perrin P and Griffiths B (eds) New Developments and New Applications in Animal Cell Technology, (pp. 555-559), Kluwer Academic Publishers, Dordrecht, The Netherlands."},{"key":"192980_CR11","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1016\/B978-0-7506-1103-9.50080-9","volume-title":"Production of Biologicals from Animal Cells in Culture","author":"J Litwin","year":"1991","unstructured":"Litwin J (1991) The growth of CHO and BHK cells as suspended aggregates in serum-free medium. In: Spier RE, Griffiths JB and Meignier B (eds) Production of Biologicals from Animal Cells in Culture, (pp. 429-433), Butterworth-Heinemann, Oxford, UK."},{"key":"192980_CR12","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1007\/BF00570893","volume":"10","author":"J Litwin","year":"1992","unstructured":"Litwin J (1992) The growth of Vero cells in suspension as cell aggregates in serum-free medium. Cytotechnol 10, 169-174.","journal-title":"Cytotechnol"},{"key":"192980_CR13","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1007\/BF00772195","volume":"14","author":"O-W Merten","year":"1994","unstructured":"Merten O-W, Kierulff KV, Castignolles N and Perrin P (1994) Evaluation of the new serum-free medium (MDSS2) for the production of different biologicals: use of various cell lines. Cytotechnol 14, 47-59.","journal-title":"Cytotechnol"},{"key":"192980_CR14","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1006\/biol.1995.0030","volume":"23","author":"O-W Merten","year":"1995","unstructured":"Merten O-W, Petres S and Couv\u00e9 E (1995) A simple serum-free freezing medium for serum-free cultured cells. Biologicals 23, 185-189.","journal-title":"Biologicals"},{"key":"192980_CR15","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/978-1-4899-1382-1_19","volume-title":"Novel Strategies in Design and Production of Vaccines","author":"O-W Merten","year":"1996","unstructured":"Merten O-W, Hannoun C, Manuguerra J-C, Ventre F and Petres S (1996) Production of influenza virus in cell cultures for vaccine preparation. In: Cohen S and Shafferman A (eds) Novel Strategies in Design and Production of Vaccines, (pp. 141-151) Plenum Press, New York, USA."},{"key":"192980_CR16","doi-asserted-by":"publisher","first-page":"35","DOI":"10.1023\/A:1007999313566","volume":"25","author":"O-W Merten","year":"1997","unstructured":"Merten O-W, Wu R, Couv\u00e9 E and Crainic R (1997) Evaluation of the serum-free medium MDSS2 for the production of poliovirus on Vero cells in bioreactors. Cytotechnol 25, 35-44.","journal-title":"Cytotechnol"},{"key":"192980_CR17","unstructured":"Merten O-W, Manuguerra J-C, Hannoun C and van der Werf S (1998) Production of influenza virus in serum-free mammalian cell cultures. In press."},{"key":"192980_CR18","first-page":"55","volume":"47","author":"BJ Montagnon","year":"1981","unstructured":"Montagnon BJ, Fanget B and Nicolas AJ (1981) The large scale cultivation of Vero cells in microcarrier culture for vaccine production. Preliminary results for killed poliovirus vaccine. Dev Biol Standard 47, 55-64.","journal-title":"Dev Biol Standard"},{"key":"192980_CR19","first-page":"37","volume":"55","author":"BJ Montagnon","year":"1984","unstructured":"Montagnon BJ, Vincent-Falquet JC and Fanget B (1984) Thousand litre scale microcarrier culture of Vero cells for killed polio virus vaccine. Promising results. Dev Biol Standard 55, 37-42.","journal-title":"Dev Biol Standard"},{"key":"192980_CR20","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1016\/B978-0-7506-1845-8.50102-2","volume-title":"Animal Cell Technology: Products of Today, Prospects for Tomorrow","author":"W No\u00e9","year":"1994","unstructured":"No\u00e9 W, Schorn P, Bux R and Berthold W (1994) Fed-batch strategies for mammalian cell cultures. In: Spier RE, Griffiths JB and Berthold W (eds) Animal Cell Technology: Products of Today, Prospects for Tomorrow, (pp. 413-418) Butterworth-Heinemann, Oxford, UK."},{"key":"192980_CR21","doi-asserted-by":"publisher","first-page":"321","DOI":"10.1016\/1045-1056(90)90037-Z","volume":"18","author":"P Perrin","year":"1990","unstructured":"Perrin P, Morgeaux S and Sureau P (1990) In vitro rabies vaccine potency appraisal by ELISA: advantages of the immunocapture method with a neutralizing anti-glycoprotein monoclonal antibody, Biologicals 18, 321-330.","journal-title":"Biologicals"},{"key":"192980_CR22","doi-asserted-by":"publisher","first-page":"1244","DOI":"10.1016\/0264-410X(94)00022-F","volume":"13","author":"P Perrin","year":"1995","unstructured":"Perrin P, Madhusudana S, Gontier-Jallet C, Petres S, Tordo N and Merten O-W (1995) An experimental rabies vaccine produced with a new BHK-21 suspension culture process: use of serum-free medium and perfusion-reactor system. Vaccine 13, 1244-1250.","journal-title":"Vaccine"},{"key":"192980_CR23","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/0022-1759(96)00080-4","volume":"194","author":"E-J Schlaeger","year":"1996","unstructured":"Schlaeger E-J (1996) The protein hydrolysate, Primatone RL, is a cost-effective multiple growth promoter of mammalian cell culture in serum-containing and serum-free media and displays anti-apoptosis properties. J Immunol Methods 194, 191-199.","journal-title":"J Immunol Methods"},{"key":"192980_CR24","first-page":"183","volume":"7","author":"E-J Schlaeger","year":"1993","unstructured":"Schlaeger E-J, Foggetta M, Vonach JM and Christensen K (1993) SF-1, a low cost culture medium for the production of recombinant proteins in baculovirus infected insect cells. Biotechnol Tech 7, 183-188.","journal-title":"Biotechnol Tech"},{"key":"192980_CR25","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/0022-1759(89)90314-1","volume":"116","author":"Y-J Schneider","year":"1989","unstructured":"Schneider Y-J (1989) Optimisation of hybridoma cell growth and monoclonal antibody secretion in a chemically defined, serum-and protein-free culture medium. J Immunol Methods 116, 65-77.","journal-title":"J Immunol Methods"},{"key":"192980_CR26","doi-asserted-by":"publisher","first-page":"259","DOI":"10.1016\/0022-1759(87)90136-0","volume":"99","author":"E Shacter","year":"1987","unstructured":"Shacter E (1987) Serum-free medium for growth factor-dependent and-independent plasmacytomas and hybridomas. J Immunol Methods 99, 259-270.","journal-title":"J Immunol Methods"},{"key":"192980_CR27","first-page":"354","volume-title":"Laboratory Techniques in Rabies","author":"JS Smith","year":"1973","unstructured":"Smith JS, Yager PA and Baer GM (1973) A rapid tissue culture test for determining rabies neutralizing antibody. In: Kaplan M-M and Koprowsky H (eds) Laboratory Techniques in Rabies, (pp. 354-357) WHO, Geneva, Switzerland."},{"key":"192980_CR28","unstructured":"WHO (1990) Manual for the virological investigation of poliomyelitis. In: \u2018Global Poliomyelitis Eradication by the Year 2000\u2019, pp. 37-40."},{"key":"192980_CR29","doi-asserted-by":"crossref","first-page":"815","DOI":"10.1007\/BF02620959","volume":"23","author":"N Yabe","year":"1987","unstructured":"Yabe N, Kato M, Matsuya T, Yamane I, Iizuka M, Takayoshi H and Suzuki K (1987) Role of iron chelators in growth-promoting effect on mouse hybridoma cells in a chemically defined medium. In Vitro Dev Biol 23, 815-820.","journal-title":"In Vitro Dev Biol"}],"container-title":["Cytotechnology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1023\/A:1008021317639.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/article\/10.1023\/A:1008021317639\/fulltext.html","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/link.springer.com\/content\/pdf\/10.1023\/A:1008021317639.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T02:11:57Z","timestamp":1752459117000},"score":1,"resource":{"primary":{"URL":"https:\/\/link.springer.com\/10.1023\/A:1008021317639"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,7]]},"references-count":29,"journal-issue":{"issue":"1-3","published-print":{"date-parts":[[1999,7]]}},"alternative-id":["192980"],"URL":"https:\/\/doi.org\/10.1023\/a:1008021317639","relation":{},"ISSN":["0920-9069","1573-0778"],"issn-type":[{"value":"0920-9069","type":"print"},{"value":"1573-0778","type":"electronic"}],"subject":[],"published":{"date-parts":[[1999,7]]}}}