{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T14:33:40Z","timestamp":1761057220231},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"5","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2000,10,1]]},"abstract":"<jats:p>Mouse carrier erythrocytes containing 125I-interleukin 3 have been prepared and treated with band 3 crosslinking reagents. The incorporation of interleukin 3 by hypotonic treatment into mouse erythrocytes reached levels of about 15% of the interleukin 3 added to the medium being predominantly present in the cytosolic fraction (73%). Uptake fell to about 7.4% when using the same conditions but omitting hypotonic shock. The interaction of band 3 crosslinked interleukin 3 loaded erythrocytes with macrophages was also studied. A high level of incorporation of interleukin 3 into macrophages was observed either from band 3 crosslinked, interleukin 3-loaded erythrocytes or from interleukin 3 loaded erythrocytes. The observations encourage the view that the system may be able to deliver and target cytokines and other growth factors to macrophages.<\/jats:p>","DOI":"10.1023\/a:1010334118492","type":"journal-article","created":{"date-parts":[[2002,12,23]],"date-time":"2002-12-23T17:41:19Z","timestamp":1040665279000},"page":"399-410","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Delivery to Macrophages of Interleukin 3 Loaded in Mouse Erythrocytes"],"prefix":"10.1042","volume":"20","author":[{"given":"Gemma","family":"Olmos","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Campus Universitario, Universidad de Alcal\u00e1 28871, Alcal\u00e1 de Henares (Madrid), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. Alfredo","family":"Lotero","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Campus Universitario, Universidad de Alcal\u00e1 28871, Alcal\u00e1 de Henares (Madrid), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Cristina","family":"Tejedor","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Campus Universitario, Universidad de Alcal\u00e1 28871, Alcal\u00e1 de Henares (Madrid), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 C.","family":"Diez","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular, Campus Universitario, Universidad de Alcal\u00e1 28871, Alcal\u00e1 de Henares (Madrid), Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Bioscience Reports"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/20\/5\/399\/470204\/bsr0200399.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/bioscirep\/article-pdf\/20\/5\/399\/470204\/bsr0200399.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,16]],"date-time":"2021-11-16T17:51:57Z","timestamp":1637085117000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/bioscirep\/article\/20\/5\/399\/54658\/Delivery-to-Macrophages-of-Interleukin-3-Loaded-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,10,1]]}},"URL":"https:\/\/doi.org\/10.1023\/a:1010334118492","relation":{},"ISSN":["0144-8463","1573-4935"],"issn-type":[{"value":"0144-8463","type":"print"},{"value":"1573-4935","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,10,1]]}}}