{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,10]],"date-time":"2024-08-10T00:04:47Z","timestamp":1723248287446},"reference-count":48,"publisher":"IMR Press","issue":"3-4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal for Vitamin and Nutrition Research"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:p> Abstract. Folate could have an antioxidant role but also may be detrimental under vitamin B<jats:sub>12<\/jats:sub> deficiency. The aim was to investigate the effect of different dietary folic acid (FA) levels, on oxidative stress in B<jats:sub>12<\/jats:sub> induced deficient aged rats. Thirty-five male aged Sprague\u2013Dawley rats, were fed either a vitamin B<jats:sub>12<\/jats:sub> deficient (n\u00a0=\u00a027) or a control diet (n\u00a0=\u00a08) during eight weeks. Then, animals were divided into four groups: B<jats:sub>12<\/jats:sub> and FA deficient diet (DBDF), B<jats:sub>12<\/jats:sub> deficient diet and FA control diet (DBCF), B<jats:sub>12<\/jats:sub> deficient diet and FA supplemented diet (DBSF), and control diet (CBCF) for a 30 days period. Methionine metabolism and antioxidant status were evaluated. Both vitamins deficiencies elevated serum homocysteine (Hcy) (7.7 vs. 4.3\u00a0\u03bcmol\/L, p\u00a0&lt;\u00a00.05) and reduced S-adenosylmethionine hepatic content (283.7 vs. 581.9\u00a0\u03bcg\/g protein, p\u00a0&lt;\u00a00.05), the total antioxidant capacity (155.7 vs. 189.3\u00a0\u03bcmol\/L, p\u00a0&lt;\u00a00.05), glutathione (GSH) (120.5 vs. 419.9\u00a0\u03bcg\/mg protein, p\u00a0&lt;\u00a00.05) and oxidized glutathione (0.9 vs. 2.6\u00a0\u03bcg\/mg protein, p\u00a0&lt;\u00a00.05) compared to control. Activities of glutathione peroxidase and glutathione reductase enzymes or damage to macromolecules were unaffected. Adequate or elevated dietary FA in B<jats:sub>12<\/jats:sub> deficiency rats decreased Hcy (5.7 and 6.3\u00a0\u03bcmol\/L, respectively) and increased total antioxidant capacity (189.8 and 192.6\u00a0\u03bcmol\/L, respectively) to values similar to control group, whereas GSH concentration was significantly lower than control (209.1 and 208.0\u00a0\u03bcg\/mg protein respectively, p\u00a0&lt;\u00a00.05). In conclusion, in a vitamin B<jats:sub>12<\/jats:sub> deficiency status, adequate or elevated FA prevented impairment in one-carbon metabolism, but does not fully reverse the decrease in antioxidant capacity. <\/jats:p>","DOI":"10.1024\/0300-9831\/a000537","type":"journal-article","created":{"date-parts":[[2019,2,12]],"date-time":"2019-02-12T15:50:31Z","timestamp":1549986631000},"page":"239-248","source":"Crossref","is-referenced-by-count":1,"title":["Adequate or elevated dietary folate does not ameliorate the reduced antioxidant capacity induced by vitamin B<sub>12<\/sub> deficiency in aged rats"],"prefix":"10.31083","volume":"90","author":[{"given":"Natalia","family":"\u00dabeda","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical and Health Sciences. 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