{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T15:30:15Z","timestamp":1778340615746,"version":"3.51.4"},"reference-count":48,"publisher":"IMR Press","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal for Vitamin and Nutrition Research"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:p> Abstract: Roux-en-Y gastric bypass (RYGB) is one of the most performed bariatric surgical techniques. However, RYGB commonly results, as side effects, in nutritional deficiencies. This study aimed to examine changes in the expression of vitamin A pathway encoding genes in the gastrointestinal tract (GI) and to evaluate the potential mechanisms associated with hypovitaminosis A after RYGB. Intestinal biopsies were obtained through double-balloon endoscopy in 20 women with obesity (age 46.9\u00b16.2 years; body mass index [BMI] 46.5\u00b15.3 kg\/m<jats:sup>2<\/jats:sup> [mean\u00b1SD]) before and three months after RYGB (BMI, 38.2\u00b14.2 kg\/m<jats:sup>2<\/jats:sup>). Intestinal mucosal gene microarray analyses were performed in samples using a Human GeneChip 1.0 ST array (Affymetrix). Vitamin A intake was assessed from 7-day food records and serum retinol levels were evaluated by electrochemiluminescence immunoassay. Our results showed the following genes with significant downregulation (p\u22640.05): LIPF (\u22120.60), NPC1L1 (\u22120.71), BCO1 (\u22120.45), and RBP4 (\u22120.13) in the duodenum; CD36 (\u22120.33), and ISX (\u22120.43) in the jejunum and BCO1 (\u22120.29) in the ileum. No significant changes in vitamin A intake were found (784\u00b1694 retinol equivalents [RE] pre-operative vs. 809\u00b1753 RE post-operative [mean\u00b1SD]). Although patients were routinely supplemented with 3500 international units IU\/day (equivalent to 1050 \u03bcg RE\/day) of oral retinol palmitate, serum concentrations were lower in the post-operative when compared to pre-operative period (0.35\u00b10.14 \u03bcg\/L vs. 0.52\u00b10.33 \u03bcg\/L, respectively \u2013 P=0.07), both within the normal range. After RYGB, the simultaneous change in expression of GI genes, may impair carotenoid metabolism in the enterocytes, formation of nascent chylomicrons and transport of retinol, resulting in lower availability of vitamin A. <\/jats:p>","DOI":"10.1024\/0300-9831\/a000767","type":"journal-article","created":{"date-parts":[[2022,9,27]],"date-time":"2022-09-27T06:37:40Z","timestamp":1664260660000},"page":"27-36","source":"Crossref","is-referenced-by-count":3,"title":["Gastrointestinal genetic reprogramming of vitamin A metabolic pathways in response of <i>Roux-en-Y<\/i> gastric bypass"],"prefix":"10.31083","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8011-0140","authenticated-orcid":true,"given":"Priscilla","family":"Sampaio","sequence":"first","affiliation":[{"name":"Centro Universit\u00e1rio S\u00e3o Camilo, S\u00e3o Paulo, Brazil"},{"name":"Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, University of S\u00e3o Paulo (LIM 35), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9196-9372","authenticated-orcid":true,"given":"Dan Linetzky","family":"Waitzberg","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, University of S\u00e3o Paulo (LIM 35), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2514-9050","authenticated-orcid":true,"given":"Natasha Mendon\u00e7a","family":"Machado","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, University of S\u00e3o Paulo (LIM 35), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raquel Susana Matos","family":"de Miranda Torrinhas","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, University of S\u00e3o Paulo (LIM 35), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Danielle C.","family":"Fonseca","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Digestive Surgery Discipline, School of Medicine, University of S\u00e3o Paulo (LIM 35), Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3324-2519","authenticated-orcid":true,"given":"Beatriz A. 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