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The aim of this randomized, double-blinded 12-week intervention study was to investigate whether Hcy levels in healthy elderly subjects (75.4\u00b14.5 years, n=133) could be lowered with a micronutrient supplement (i.e., 400 \u03bcg folic acid, 100 \u03bcg cobalamin). Difference in mean initial Hcy levels between intervention (17.6\u00b17.1 \u03bcmol\/L, n=65) and placebo group (18.9\u00b16.1 \u03bcmol\/L, n=68) was not significant. The prevalence of cobalamin and folate deficiency in the total study population was low: 27% had serum-cobalamin levels \u2264150 pmol\/L, 12% holo-transcobalamin (Holo-TC) levels \u226450 pmol\/L, 13% low cobalamin status using the aggregated cobalamin marker 4cB12 and 10% red blood cell (RBC) folate \u2264570 nmol\/L. Nevertheless, the treated subjects still showed improved cobalamin and folate biostatus (serum cobalamin \u0394t<jats:sub>12<\/jats:sub>-t<jats:sub>0<\/jats:sub>: 63\u00b148 pmol\/L; Holo-TC \u0394t<jats:sub>12<\/jats:sub>-t<jats:sub>0<\/jats:sub>: 17\u00b119 pmol\/L; RBC folate \u0394t<jats:sub>12<\/jats:sub>-t<jats:sub>0<\/jats:sub>: 326\u00b1253 nmol\/L) and Hcy levels (\u0394t<jats:sub>12<\/jats:sub>-t<jats:sub>0<\/jats:sub>: \u22123.6\u00b15.7 \u03bcmol\/L). The effects were statistically significant compared to the placebo group with p=0.005 (serum cobalamin), p=0.021 (Holo-TC), p=0.014 (RBC-folate) and p&lt;0.001 (Hcy). The Hcy-lowering effect was dependent on the initial Hcy levels (p&lt;0.001). Our findings suggest that elevated Hcy levels in elderly subjects can be lowered regardless of the initial cobalamin and folate biostatus. <\/jats:p>","DOI":"10.1024\/0300-9831\/a000777","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T12:46:19Z","timestamp":1675082779000},"page":"120-132","source":"Crossref","is-referenced-by-count":7,"title":["A micronutrient supplement modulates homocysteine levels regardless of vitamin B biostatus in elderly subjects"],"prefix":"10.31083","volume":"94","author":[{"given":"Marija","family":"Savic-Hartwig","sequence":"first","affiliation":[{"name":"Institute of Food Science and Human Nutrition, Leibniz University Hannover, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felix","family":"Kerlikowsky","sequence":"additional","affiliation":[{"name":"Institute of Food Science and Human Nutrition, Leibniz University Hannover, 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