{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T10:21:57Z","timestamp":1777285317855,"version":"3.51.4"},"reference-count":55,"publisher":"IMR Press","issue":"3-4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal for Vitamin and Nutrition Research"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:p> Abstract: Background: Carotenoids have been reported to exert protective effects against age-related diseases via changes in DNA methylation. Although lower thioredoxin-interacting protein (TXNIP) DNA methylation is associated with age-related diseases, only a few studies have investigated the factors influencing TXNIP DNA methylation. Carotenoids may be a factor linking TXNIP to specific pathophysiological functions. The aim of this study was to examine whether serum carotenoid levels are associated with TXNIP DNA methylation levels. Methods: We conducted a cross-sectional study using 376 health examination participants (169 men). DNA methylation levels were determined using a pyrosequencing assay. Serum carotenoid levels were determined by high-performance liquid chromatography. Multivariable regression analyses were performed to examine the associations between TXNIP DNA methylation levels and serum carotenoid levels with adjustment for age, BMI, HbA1c, CRP, smoking habits, alcohol consumption, exercise habits, and percentage of neutrophils. Results: Multiple linear regression analyses showed that TXNIP DNA methylation levels were positively associated with serum levels of zeaxanthin\/lutein ( \u03b2 [95%CI]: 1.935 [0.184, 3.685]), \u03b2-cryptoxanthin (1.447 [0.324, 2.570]), \u03b1-carotene (1.061 [0.044, 2.077]), \u03b2-carotene (1.272 [0.319, 2.226]), total carotenes (1.255 [0.040, 2.469]), total xanthophylls (2.133 [0.315, 3.951]), provitamin A (1.460 [0.402, 2.519]), and total carotenoids (1.972 [0.261, 3.683]) in men (all p&lt;0.05). Of these, provitamin A showed the stronger association (standardized \u03b2=0.216). No significant association of TXNIP DNA methylation and serum carotenoid was observed in women. Conclusions: The findings of this study suggest that carotenoid intake may protect against age-related diseases by altering TXNIP DNA methylation status in men. <\/jats:p>","DOI":"10.1024\/0300-9831\/a000791","type":"journal-article","created":{"date-parts":[[2023,9,22]],"date-time":"2023-09-22T06:21:11Z","timestamp":1695363671000},"page":"210-220","source":"Crossref","is-referenced-by-count":4,"title":["Serum carotenoid levels are positively associated with DNA methylation of thioredoxin-interacting protein"],"prefix":"10.31083","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8916-2687","authenticated-orcid":true,"given":"Keisuke","family":"Maeda","sequence":"first","affiliation":[{"name":"Department of Clinical Physiology, Fujita Health University School of Medical Sciences, Toyoake, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroya","family":"Yamada","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Fujita Health University School of Medicine, Toyoake, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eiji","family":"Munetsuna","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Fujita Health University School of Medicine, Toyoake, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1730-2059","authenticated-orcid":true,"given":"Ryosuke","family":"Fujii","sequence":"additional","affiliation":[{"name":"Department of Preventive Medical Sciences, Fujita Health University School of Medical Sciences, Toyoake, Japan"},{"name":"Institute for Biomedicine (affiliated to the University of L\u00fcbeck), Eurac Research, Bolzano\/Bozen, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8170-9027","authenticated-orcid":true,"given":"Mirai","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, Kagawa Prefectural University of Health Sciences, Takamatsu, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1213-8096","authenticated-orcid":true,"given":"Yoshitaka","family":"Ando","sequence":"additional","affiliation":[{"name":"Department of Informative Clinical Medicine, Fujita Health University School of Medical Sciences, Toyoake, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Genki","family":"Mizuno","sequence":"additional","affiliation":[{"name":"Department of Medical Technology, School of Health Sciences, Tokyo University of Technology, Tokyo, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8145-3949","authenticated-orcid":true,"given":"Yoshiki","family":"Tsuboi","sequence":"additional","affiliation":[{"name":"Department of Preventive Medical Sciences, Fujita Health University School of Medical Sciences, Toyoake, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Informative Clinical Medicine, Fujita Health University School of Medical Sciences, Toyoake, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Ohashi","sequence":"additional","affiliation":[{"name":"Department of Informative Clinical Medicine, Fujita Health University School of Medical Sciences, Toyoake, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuji","family":"Hashimoto","sequence":"additional","affiliation":[{"name":"Department of Hygiene, Fujita Health University School of Medicine, Toyoake, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuyuki","family":"Hamajima","sequence":"additional","affiliation":[{"name":"Department of Healthcare Administration, Nagoya University Graduate School of Medicine, Nagoya, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Preventive Medical Sciences, Fujita Health University School of Medical Sciences, Toyoake, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"16242","reference":[{"key":"c1","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.1999.10718880"},{"key":"c2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mam.2005.10.001"},{"issue":"6","key":"c3","first-page":"493","volume":"3","author":"Campbell DR","year":"1994","journal-title":"Cancer Epidemiol Biomarkers Prev"},{"key":"c4","doi-asserted-by":"crossref","unstructured":"Burrows TL, Williams R, Rollo M, Wood L, Garg ML, Jensen M, et al. 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