{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T19:11:56Z","timestamp":1726427516174},"reference-count":0,"publisher":"Hogrefe Publishing Group","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vasa"],"published-print":{"date-parts":[[1999,5,1]]},"abstract":"<jats:p> Background: These experiments aimed to study the in vivo short and long term neurovascular regeneration after frostbite. Methods: The rabbit central ear-artery was used as the experimental model. The effects on the noradrenergic innervation of the artery were measured in isolated vascular ring segments the first day and 2, 3\u20134, and 8\u201310 or 10\u201320 weeks following freezing at \u20139\u00b0C or \u201318\u00b0C for 15 min with slow rewarming for 7 min at room temperature. Results: Two days after freezing the sympathetic nerves were completely degenerated, as observed with glyoxylic acid-induced fluorescence. The vascular isometric tension responses to exogenous noradrenaline and endogenously released noradrenaline by electrical stimulation in vitro were abolished. A varying degree of necrosis of the vascular wall was observed. Two weeks after freezing at \u201318\u00b0C in vitro responses to exogenous noradrenaline and electrical stimulation were still abolished, then gradually approaching control levels after 10\u201320 weeks of in vivo regeneration. Eight and 10 weeks after injury at \u20139\u00b0C increased vascular tension responses to exogenous noradrenaline was found. In spite of a long regeneration period the total uptake and the spontaneous and K<jats:sup>+<\/jats:sup> (75 mM) evoked releases of [<jats:sup>3<\/jats:sup>H]noradrenaline were persistently decreased after frostbite at \u201318\u00b0C, but they were regenerated to control levels already 10\u201320 weeks after \u20139\u00b0C. Regeneration of noradrenergic nerve function, expressed as [<jats:sup>3<\/jats:sup>H]noradrenaline uptake and release and responsiveness to electrical stimulation, expressed as vascular contraction, was slower than the regeneration of the vascular smooth muscle. Myointimal hyperplasia developed in response to \u20139\u00b0C and \u201318\u00b0C frostbite. The uptake and the K<jats:sup>+<\/jats:sup> evoked release of [3H]noradrenaline were particularly sensitive parameters for autonomic nerve function. Conclusions: The present findings may demonstrate important neurovascular reactions to local frostbite and may explain human sequelae following frostbite <\/jats:p>","DOI":"10.1024\/0301-1526.28.2.85","type":"journal-article","created":{"date-parts":[[2006,1,31]],"date-time":"2006-01-31T12:25:05Z","timestamp":1138710305000},"page":"85-94","source":"Crossref","is-referenced-by-count":4,"title":["Fr\u00fche und sp\u00e4te funktionelle und histopathologische St\u00f6rungen nach K\u00e4lteschaden im Tierexperiment"],"prefix":"10.1024","volume":"28","author":[{"family":"Arvesen","sequence":"first","affiliation":[{"name":"Division for Environmental Toxicology, Norwegian Defence Research Establishment, Kjeller, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"M\u00e6hlen","sequence":"additional","affiliation":[{"name":"Ullev\u00e5l University Hospital, Oslo, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Ros\u00e9n","sequence":"additional","affiliation":[{"name":"Norwegian Army Medical Regiment, Skjetten, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"family":"Aas","sequence":"additional","affiliation":[{"name":"Division for Environmental Toxicology, Norwegian Defence Research Establishment, Kjeller, Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"101","container-title":["Vasa"],"original-title":[],"language":"de","link":[{"URL":"https:\/\/econtent.hogrefe.com\/doi\/pdf\/10.1024\/0301-1526.28.2.85","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,5,8]],"date-time":"2022-05-08T10:49:47Z","timestamp":1652006987000},"score":1,"resource":{"primary":{"URL":"https:\/\/econtent.hogrefe.com\/doi\/10.1024\/0301-1526.28.2.85"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1999,5,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1999,5,1]]}},"alternative-id":["10.1024\/0301-1526.28.2.85"],"URL":"https:\/\/doi.org\/10.1024\/0301-1526.28.2.85","relation":{},"ISSN":["0301-1526","1664-2872"],"issn-type":[{"type":"print","value":"0301-1526"},{"type":"electronic","value":"1664-2872"}],"subject":[],"published":{"date-parts":[[1999,5,1]]}}}