{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T17:05:22Z","timestamp":1764349522668},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,1,11]],"date-time":"2002-01-11T00:00:00Z","timestamp":1010707200000},"content-version":"vor","delay-in-days":681,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:p>Guanine nucleotide dissociation inhibitor (GDI) is a 55\u2010kDa protein that functions in vesicular membrane transport to recycle Rab GTPases. We have now determined the crystal structure of bovine \u03b1\u2010GDI at ultra\u2010high resolution (1.04 \u00c5). Refinement at this resolution highlighted a region with high mobility of its main\u2010chain residues. This corresponded to a surface loop in the primarily \u03b1\u2010helical domain II at the base of \u03b1\u2010GDI containing the previously uncharacterized sequence\u2010conserved region (SCR) 3A. Site\u2010directed mutagenesis showed that this mobile loop plays a crucial role in binding of GDI to membranes and extraction of membrane\u2010bound Rab. This domain, referred to as the mobile effector loop, in combination with Rab\u2010binding residues found in the multi\u2010sheet domain I at the apex of \u03b1\u2010GDI may provide flexibility for recycling of diverse Rab GTPases. We propose that conserved residues in domains I and II synergize to form the functional face of GDI, and that domain II mediates a critical step in Rab recycling during vesicle fusion.<\/jats:p>","DOI":"10.1034\/j.1600-0854.2000.010309.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T22:10:52Z","timestamp":1047507052000},"page":"270-281","source":"Crossref","is-referenced-by-count":35,"title":["A New Functional Domain of Guanine Nucleotide Dissociation Inhibitor (\u03b1\u2010GDI) Involved in Rab Recycling"],"prefix":"10.1034","volume":"1","author":[{"given":"Peng","family":"Luan","sequence":"first","affiliation":[]},{"given":"Andreas","family":"Heine","sequence":"additional","affiliation":[]},{"given":"Ke","family":"Zeng","sequence":"additional","affiliation":[]},{"given":"Bryan","family":"Moyer","sequence":"additional","affiliation":[]},{"given":"Samantha E.","family":"Greasely","sequence":"additional","affiliation":[]},{"given":"Peter","family":"Kuhn","sequence":"additional","affiliation":[]},{"given":"William E.","family":"Balch","sequence":"additional","affiliation":[]},{"given":"Ian A.","family":"Wilson","sequence":"additional","affiliation":[]}],"member":"311","published-online":{"date-parts":[[2002,1,11]]},"container-title":["Traffic"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0854.2000.010309.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0854.2000.010309.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T17:06:17Z","timestamp":1698599177000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0854.2000.010309.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["10.1034\/j.1600-0854.2000.010309.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0854.2000.010309.x","archive":["Portico"],"relation":{},"ISSN":["1398-9219","1600-0854"],"issn-type":[{"value":"1398-9219","type":"print"},{"value":"1600-0854","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3]]}}}