{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:43:19Z","timestamp":1779345799802,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,12,21]],"date-time":"2001-12-21T00:00:00Z","timestamp":1008892800000},"content-version":"vor","delay-in-days":295,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:p><jats:italic>Leishmania<\/jats:italic> are a group of primitive eukaryotic trypanosomatid protozoa that are apically polarized with a flagellum at their anterior end. Surrounding the base of the flagellum is the flagellar reservoir that constitutes the site for endocytosis and exocytosis in these organisms. In the present study, we define a novel multivesicular tubular compartment involved in the intracellular trafficking of macromolecules in <jats:italic>Leishmania<\/jats:italic>. This dynamic structure appears to subtend the flagellar reservoir and extends towards the posterior end of the cell. Functional domains of several surface\u2010expressed proteins, such as the gp63 glycosyl phosphatidyl inositol anchor and the 3\u2032nucleotidase\/nuclease transmembrane domain were fused to green fluorescent protein. These chimeric proteins were found to traffic through the secretory pathway and, while reaching their intended destinations, also accumulated within the intracellular tubular compartment. Using various compounds that are efficient fluid\u2010phase markers used to track endocytosis in higher eukaryotes, we showed that this tubular compartment constitutes an important station in the endocytic pathway of these cells. Based on our functional observations of its role in the trafficking of expressed proteins and endocytosed markers, this compartment appears to have properties similar to endosomes of higher eukaryotes.<\/jats:p>","DOI":"10.1034\/j.1600-0854.2001.020304.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T22:12:41Z","timestamp":1047507161000},"page":"175-188","source":"Crossref","is-referenced-by-count":54,"title":["Secretory and Endocytic Pathways Converge in a Dynamic Endosomal System in a Primitive Protozoan"],"prefix":"10.1111","volume":"2","author":[{"given":"Elodie","family":"Ghedin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alain","family":"Debrabant","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan C.","family":"Engel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis M.","family":"Dwyer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,21]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(93)90008-4"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M908725199"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(93)90106-8"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0309-1651(91)90034-G"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.112.17.2799"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.36.22997"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(95)00035-y"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(89)90115-X"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/340167a0"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.46.100192.000433"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0962-8924(97)10046-0"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.100.1.27"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.113.6.1267"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(97)80029-4"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.60.4.697-721.1996"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5424.63"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90027-A"},{"key":"e_1_2_6_19_2","first-page":"502","article-title":"Fusion of host cell secondary lysosomes with the parasitophorous vacuoles of Leishmania infected macrophages","volume":"22","author":"Alexander J","year":"1975","journal-title":"J Parasitol"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000063411"},{"key":"e_1_2_6_21_2","doi-asserted-by":"crossref","first-page":"9464","DOI":"10.1074\/jbc.272.14.9464","article-title":"Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to virulence in Leishmania mexicana amazonensis amastigotes","volume":"14","author":"Lu H\u2010L","year":"1997","journal-title":"J Biol Chem"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.13.3643"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(90)90063-R"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.107.11.3191"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.20.11015"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3350681"},{"key":"e_1_2_6_27_2","first-page":"215","volume-title":"Microbiology and Microbial Infections, vol. 15. Parasitology","author":"Ashford RW","year":"1998"},{"key":"e_1_2_6_28_2","doi-asserted-by":"crossref","first-page":"1345","DOI":"10.1242\/jcs.113.8.1345","article-title":"Up\u2010regulation of the secretory pathway in cysteine protease inhibitor\u2010resistant Trypanosoma cruzi","volume":"113","author":"Engel JC","year":"2000","journal-title":"J Cell Sci"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0166-6851(96)02612-6"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.9.1.173"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1998.4198"}],"container-title":["Traffic"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0854.2001.020304.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0854.2001.020304.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T08:24:21Z","timestamp":1697531061000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0854.2001.020304.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,3]]},"references-count":30,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,3]]}},"alternative-id":["10.1034\/j.1600-0854.2001.020304.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0854.2001.020304.x","archive":["Portico"],"relation":{},"ISSN":["1398-9219","1600-0854"],"issn-type":[{"value":"1398-9219","type":"print"},{"value":"1600-0854","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,3]]}}}