{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:09:05Z","timestamp":1762099745266},"reference-count":26,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":510,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:p> <jats:bold>Background:<\/jats:bold> Airway allergen exposure causes local eosinophilic cell infiltration. This cellular inflammatory response is likely to involve the release of eosinophils from peripheral storage pools, and possibly also regeneration of eosinophils in the bone marrow. IL\u201012 is an inhibitory cytokine in allergic inflammation, shown to reduce eosinophilic cell infiltration. The aim of the present study was to determine whether airway allergen exposure increases bone marrow eosinophil production, and, if so, whether IL\u201012 modulates this effect.<\/jats:p><jats:p> <jats:bold>Methods:<\/jats:bold> Ovalbumin\u2010sensitized C57BL\/6 mice and IL\u201012 knockout (KO) mice were exposed to allergen via the airway route, and the inflammatory cell response was evaluated in bronchoalveolar lavage fluid, blood, and bone marrow.<\/jats:p><jats:p> <jats:bold>Results:<\/jats:bold> Allergen instillation intranasally produced a dose\u2010dependent inflammatory response in the lower airways of sensitized mice. This inflammatory response was dominated by eosinophils, but there were also increases of both lymphocytes and neutrophils. Sensitization and airway allergen exposure also increased the IL\u20105\u2010dependent growth of bone marrow cells <jats:italic>in vitro<\/jats:italic>. The enhanced bone marrow responsiveness <jats:italic>in vitro<\/jats:italic> was paralleled by an increased number of bone marrow eosinophils <jats:italic>in vivo<\/jats:italic>. After sensitization and repeated allergen exposure, IL\u201012\u2003KO mice showed higher eosinophil levels in both BAL and bone marrow than parallel wild\u2010type control mice. Furthermore, BAL\u2010eotaxin levels were increased in IL\u201012\u2003KO mice as opposed to parallel wild\u2010type controls after allergen exposure.<\/jats:p><jats:p> <jats:bold>Conclusions:<\/jats:bold> Airway allergen exposure induced systemic immunologic responses, including increased eosinophil numbers in both airways and bone marrow, and also enhanced IL\u20105 responsiveness in bone marrow cells. IL\u201012 may regulate airway eosinophilia at both the level of eosinophilopoiesis and the level of local recruitment of eosinophils into the airways.<\/jats:p>","DOI":"10.1034\/j.1398-9995.2000.00583.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:44:07Z","timestamp":1047347047000},"page":"749-756","source":"Crossref","is-referenced-by-count":23,"title":["IL\u201012 regulates bone marrow eosinophilia and airway eotaxin levels induced by airway allergen 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