{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:32:12Z","timestamp":1791048732168,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":449,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:p> <jats:bold>Background:<\/jats:bold> We report on a 37\u2010year\u2010old man without history of previous allergic disease who developed an aseptic intolerance reaction to a chromium\u2010cobalt alloy, with local discomfort, loosening, and absence of fracture healing. Both <jats:italic>in vivo <\/jats:italic>and <jats:italic>in vitro <\/jats:italic>allergoimmunologic diagnostic tests were performed.<\/jats:p><jats:p> <jats:bold>Methods:<\/jats:bold> Patch testing was done with a European standard series. Specific serum IgE was measured by CAP\u2010FEIA. In addition to immunohistology (APAAP method), peri\u2010implantar tissue was further analyzed by PCR to determine T\u2010cell\u2010receptor\u2010\u03b3 rearrangement and thus the potential clonal (antigen\u2010driven) T\u2010cell repertoire. The actual tissue mRNA expression for IL\u20104, IL\u20106, and IFN\u2010\u03b3 was visualized by RT\u2010PCR.<\/jats:p><jats:p> <jats:bold>Results:<\/jats:bold> Skin testing gave a delayed\u2010type reaction to dichromate. Specific serum IgE to natural rubber latex and grass pollen was found \u2013 but without clinical symptoms. Immunohistology revealed a monocytic and dense T\u2010cell infiltrate. The latter, instead of being random, showed an oligoclonal T\u2010cell receptor rearrangement. In addition, there was TH<jats:sub>1<\/jats:sub>\u2010type mediator expression (IL\u20106 and IFN\u2010\u03b3, but not IL\u20104).<\/jats:p><jats:p> <jats:bold>Conclusions:<\/jats:bold> Skin test, examination of peri\u2010implantar tissue, and the prompt healing after replacement of the osteosynthesis material suggest an allergic reaction. PCR analysis of peri\u2010implantar tissue can further help to identify and understand allergy\u2010mediated implant intolerance reactions.<\/jats:p>","DOI":"10.1034\/j.1398-9995.2000.00619.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:41:44Z","timestamp":1047346904000},"page":"969-972","source":"Crossref","is-referenced-by-count":88,"title":["Intolerance of osteosynthesis material: evidence of dichromate contact allergy with concomitant oligoclonal T\u2010cell infiltrate and TH<sub>1<\/sub>\u2010type cytokine expression in the peri\u2010implantar tissue"],"prefix":"10.1111","volume":"55","author":[{"given":"P.","family":"Thomas","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Summer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. A.","family":"Sander","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Przybilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Thomas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Naumann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007611-197304000-00013"},{"key":"e_1_2_7_3_2","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1001\/archderm.1981.01650090036021","article-title":"Cutaneous complications of orthopedic implants","volume":"117","author":"Kubba R","year":"1981","journal-title":"Arch Dermatol"},{"key":"e_1_2_7_4_2","first-page":"108","article-title":"Systemic contact allergy reactions","volume":"6","author":"Menne T","year":"1987","journal-title":"Semin Dermatol"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1985.tb02581.x"},{"key":"e_1_2_7_6_2","first-page":"91","article-title":"Does the immune system play a role in loosening and osteolysis of total joint replacement?","volume":"6","author":"Goodman SB","year":"1996","journal-title":"J Long Term Eff Med Implants"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.4.5570.36"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.3109\/17453677708988763"},{"key":"e_1_2_7_9_2","doi-asserted-by":"crossref","first-page":"862","DOI":"10.2106\/00004623-197759070-00004","article-title":"Metal sensitivity before and after total hip arthroplasty","volume":"59","author":"Deutmann R","year":"1977","journal-title":"J Bone Joint Surg"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.3109\/17453678008990769"},{"key":"e_1_2_7_11_2","first-page":"1271","article-title":"Prospective investigation of metal allergy caused by metallic implants","volume":"123","author":"Duchna HW","year":"1998","journal-title":"Zentralbl Chir"},{"key":"e_1_2_7_12_2","first-page":"62","article-title":"Implantation of orthopaedic devices in patients with metal allergy","volume":"69","author":"Carlsson A","year":"1989","journal-title":"Acta Derm Venereol (Stockh)"},{"key":"e_1_2_7_13_2","first-page":"210","article-title":"Systemically induced (hematogenous) contact eczema","volume":"9","author":"Klaschka F","year":"1990","journal-title":"Semin Dermatol"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1994.tb01951.x"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1006\/faat.1996.2279"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12476328"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.1.5852.527"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100070302"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbm.820230804"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-199608001-00020"},{"key":"e_1_2_7_21_2","first-page":"85","article-title":"Nickel allergic patients with or without reactions upon oral nickel challenge: lymphocyte reactivity and cytokine pattern","volume":"103","author":"Thomas P","year":"1999","journal-title":"J Allergy Clin Immunol"},{"key":"e_1_2_7_22_2","first-page":"29","article-title":"Automated high resolution polymerase chain reaction fragment analysis: a method for detecting T\u2010cell\u2010receptor gamma\u2010chain rearrangements in lymphoproliferative diseases","volume":"152","author":"Simon M","year":"1998","journal-title":"Am J Pathol"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1398-9995.2000.00619.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1398-9995.2000.00619.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T07:49:47Z","timestamp":1698565787000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1398-9995.2000.00619.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10]]},"references-count":21,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2000,10]]}},"alternative-id":["10.1034\/j.1398-9995.2000.00619.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1398-9995.2000.00619.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,10]]}}}