{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T05:39:13Z","timestamp":1778218753331,"version":"3.51.4"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2001,12,24]],"date-time":"2001-12-24T00:00:00Z","timestamp":1009152000000},"content-version":"vor","delay-in-days":449,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:p> <jats:bold>Background<\/jats:bold>: Allergy to almonds has been frequently reported, but data on the identification of the almond allergens, as well as on the reliability of the methods for <jats:italic>in vitro<\/jats:italic> detection of specific IgE for these allergens, are scant. This study aimed to identify the almond allergens and to evaluate the reliability of the CAP\u2010FEIA as the standard system for detection of almond\u2010specific IgE with clinical significance.<\/jats:p><jats:p> <jats:bold>Methods<\/jats:bold>: Immunoblotting performed with an almond\u2010protein extract was carried out on the sera of five patients who had previously been tested by the CAP\u2010FEIA system; two of these patients had tested negative with the CAP\u2010FEIA system but suffered life\u2010threatening laryngeal edema after eating almonds, whereas the other three subjects, who had tested positive with CAP\u2010FEIA, did not present any symptoms subsequent to almond ingestion.<\/jats:p><jats:p> <jats:bold>Results<\/jats:bold>: The sera of the two symptomatic CAP\u2010FEIA\u2010negative patients had IgE that bound only to a 37\u2010kDa protein in immunoblotting. On the contrary, the sera of the three asymptomatic subjects all showed IgE binding to two almond proteins of 62 and 50\u2003kDa, corresponding to the glycosylated components of the extract.<\/jats:p><jats:p> <jats:bold>Conclusions<\/jats:bold>: The results here presented suggest that, at least for the examined subjects, the positivity to almond, as measured with a standard laboratory method, is due to the presence of the 62\/50\u2010kDa glycoproteins with little or no immunologic significance, and not to the binding to the 37\u2010kDa polypeptide, which appears to be a true almond allergen.<\/jats:p>","DOI":"10.1034\/j.1398-9995.2000.00663.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:41:44Z","timestamp":1047346904000},"page":"955-958","source":"Crossref","is-referenced-by-count":20,"title":["IgE binding to almond proteins in two CAP\u2010FEIA\u2010negative patients with allergic symptoms to almond as comparedto three CAP\u2010FEIA\u2010false\u2010positive subjects"],"prefix":"10.1111","volume":"55","author":[{"given":"G.","family":"Pasini","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Simonato","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Giannattasio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Gemignani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Curioni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,24]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(89)90083-3"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.312.7038.1074"},{"key":"e_1_2_5_4_2","doi-asserted-by":"crossref","first-page":"S119","DOI":"10.1080\/10408399609527762","article-title":"Food allergens","volume":"36","author":"Bush RK","year":"1996","journal-title":"Crit Rev Food Sci Nutr"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.1992.tb08080.x"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(84)90083-6"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_5_8_2","first-page":"200","article-title":"Immunological distinction between x\u2010type and y\u2010type high molecular weight glutenin subunits","volume":"68","author":"Curioni A","year":"1991","journal-title":"Cereal Chem"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1522-2683(19990301)20:3<491::aid-elps491>3.0.co;2-f"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00491.x"},{"key":"e_1_2_5_11_2","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1002\/elps.1150120502","article-title":"Barley cultivar discrimination. 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Sodium dodecyl sulfate\u2010polyacrylamide gel electrophoresis and glycoprotein blotting","volume":"12","author":"Weiss W","year":"1991","journal-title":"Electrophoresis"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/(sici)1097-0010(199812)78:4<511::aid-jsfa148>3.0.co;2-x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/S0091-6749(97)70245-8","article-title":"Poor biological activity of cross\u2010reactive IgE directed to carbohydrate determinants of glycoproteins","volume":"100","author":"Van Der Ween M","year":"1997","journal-title":"J Allergy Clin Immunol"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70169-7"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70171-5"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70196-X"}],"container-title":["Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1398-9995.2000.00663.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1398-9995.2000.00663.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T07:50:33Z","timestamp":1698565833000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1398-9995.2000.00663.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10]]},"references-count":15,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2000,10]]}},"alternative-id":["10.1034\/j.1398-9995.2000.00663.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1398-9995.2000.00663.x","archive":["Portico"],"relation":{},"ISSN":["0105-4538","1398-9995"],"issn-type":[{"value":"0105-4538","type":"print"},{"value":"1398-9995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,10]]}}}