{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T20:47:25Z","timestamp":1761511645604},"reference-count":27,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,4,23]],"date-time":"2002-04-23T00:00:00Z","timestamp":1019520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:p> <jats:bold>Background:\u2002<\/jats:bold>Many patients with vernal keratoconjunctivitis (VKC), a severe chronic allergic eye disease in children, exhibit IgE\u2010sensitization, but about 40% of cases lack this immunologic trait. As a disease factor in VKC, IgE is thus not fully understood. The aim of this study was to investigate whether there are any differences in the conjunctival cytokine messenger (m)RNA pattern related to IgE\u2010sensitization in children suffering from VKC.<\/jats:p><jats:p> <jats:bold>Methods:\u2002<\/jats:bold>Tissue samples were obtained from 16 symptomatic VKC subjects with sub\u2010tarsal disease and six control subjects. Expression of mRNA for interleukin (IL)\u20104, IL\u20105, IL\u201013, and interferon (IFN)\u2010\u03b3 was investigated with a semiquantitative reverse\u2010transcriptase polymerase chain reaction (RT\u2010PCR) technique. The presence of T\u2003cells, IgE<jats:sup>+<\/jats:sup> cells, mast cells, and eosinophils was analyzed with immunohistochemical methods. Allergen\u2010specific IgE antibodies were assessed in serum and with skin prick testing.<\/jats:p><jats:p> <jats:bold>Results:\u2002<\/jats:bold>Ten out of the 16 VKC subjects showed evidence of IgE\u2010sensitization. No differences were detected for any tissue variable between VKC subjects with and without IgE\u2010sensitization. Statistically significant increases over controls were found for both VKC groups with regard to all cell markers.<\/jats:p><jats:p> <jats:bold>Conclusions:\u2002<\/jats:bold>The amount of messenger RNA encoding cytokines and inflammatory cell markers in VKC did not correlate with IgE\u2010sensitization. Tissue changes in all patient samples were characterized by a prevalence of T\u2003cells, eosinophils, mast cells and cell\u2010bound IgE molecules. However, the role of cell\u2010bound IgE molecules in VKC patients lacking IgE\u2010sensitization remains to be determined.<\/jats:p>","DOI":"10.1034\/j.1398-9995.2002.13375.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:49:29Z","timestamp":1047347369000},"page":"436-441","source":"Crossref","is-referenced-by-count":27,"title":["Similar T\u2003helper Th2\u2010like cytokine mRNA expression in vernal keratoconjunctivitis regardless of atopic constitution"],"prefix":"10.1111","volume":"57","author":[{"given":"P. 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