{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T23:47:25Z","timestamp":1769903245929,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2002,10,2]],"date-time":"2002-10-02T00:00:00Z","timestamp":1033516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:p><jats:bold>Background<\/jats:bold> Mizolastine is a potent and selective H<jats:sub>1<\/jats:sub>\u2010receptor antagonist with antiallergic properties; in in\u2010vitro animal models, mizolastine was shown to inhibit 5\u2010lipoxygenase activity and to decrease the release of leukotrienes (LT) and tumor necrosis factor\u2010\u03b1 (TNF\u2010\u03b1). This study investigated the effects of three concentrations of mizolastine (0.1, 1.0, 10\u2003\u00b5M) on the release of LT (LTB<jats:sub>4<\/jats:sub> and LTC<jats:sub>4<\/jats:sub>\/D<jats:sub>4<\/jats:sub>) and prostaglandin D<jats:sub>2<\/jats:sub> (PGD<jats:sub>2<\/jats:sub>) after stimulation by anti\u2010IgE, and on the spontaneous release of cytokines (TNF\u2010\u03b1 and granulocyte\/macrophage\u2010colony\u2010stimulating factor [GM\u2010CSF]), from dispersed cells obtained from surgically resected nasal polyps of patients with nasal polyposis.<\/jats:p><jats:p><jats:bold>Methods<\/jats:bold> Cells from nasal polyps were obtained using enzymatic dispersion. For experiments involving the measurement of LT and PGD<jats:sub>2<\/jats:sub>, the cells were preincubated with mizolastine or its dissolution vehicle for 20\u2003min prior to challenge with 10\u2003\u00b5g\/ml \u03b5\u2010chain specific anti\u2010IgE for 45\u2003min at 37\u00b0C; for the cytokine release, cells were incubated with mizolastine or its dissolution vehicle for 24\u2003h. LT and PGD<jats:sub>2<\/jats:sub> were measured by enzyme immunoassay (EIA) and cytokines by enzyme\u2010linked immunosorbent assay (ELISA) using commercially available kits.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold> Mizolastine inhibited significantly and in a dose\u2010dependent manner the release of LTB<jats:sub>4<\/jats:sub> and TNF\u2010\u03b1 at all concentrations, LTC<jats:sub>4<\/jats:sub>\/D<jats:sub>4<\/jats:sub> at 10\u2003\u00b5M, and GM\u2010CSF from 1\u2003\u00b5M; no effect was observed on the release of PGD<jats:sub>2<\/jats:sub>.<\/jats:p><jats:p><jats:bold>Conclusion<\/jats:bold> Mizolastine inhibits the release of LT, TNF\u2010\u03b1 and GM\u2010CSF in this <jats:italic>in vitro<\/jats:italic> model, which mimics closely the inflammatory cells of allergic rhinitis.<\/jats:p>","DOI":"10.1034\/j.1398-9995.2002.23452.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:48:19Z","timestamp":1047347299000},"page":"1067-1070","source":"Crossref","is-referenced-by-count":24,"title":["Inhibition of mediator and cytokine release from dispersed nasal polyp cells by 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