{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:39:47Z","timestamp":1790923187547,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2002,10,2]],"date-time":"2002-10-02T00:00:00Z","timestamp":1033516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> IgE\u2010mediated allergic reactions to bullfrog and edible frog have been reported. The implicated allergens have not been defined so far. The frog material and the patient's serum from a case of severe food\u2010induced anaphylaxis were used to define the implicated allergen at the protein and DNA level.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Immunoblotting techniques and <jats:italic>N<\/jats:italic>\u2010terminal protein microsequencing were used to define the allergen recognized by the patient's serum. Back translation from the identified protein sequence was used to design degenerated primers to amplify the allergen's cDNA by polymerase chain reaction (PCR). We defined the nucleotide sequence of the allergen from the frog of Indonesian origin that was consumed by the patient, and the homologous cDNA from <jats:italic>Rana esculenta<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Protein microsequencing revealed that the implicated frog allergen belonged to the parvalbumin family. cDNAs coding for \u03b1\u2010 and \u03b2\u2010parvalbumin of <jats:italic>R.\u2003esculenta<\/jats:italic> and <jats:italic>Rana species<\/jats:italic> were cloned. Recombinant proteins were expressed in <jats:italic>Escherichia coli<\/jats:italic>. The patient's serum IgE antibodies recognized parvalbumin prepared from frog muscle and recombinant \u03b1\u2010parvalbumin from <jats:italic>R.\u2003species<\/jats:italic> but not from <jats:italic>R.\u2003esculenta<\/jats:italic>. Recombinant \u03b2\u2010parvalbumin was not recognized by the IgE antibodies.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> This work defines at the protein and DNA levels \u03b1\u2010parvalbumin as the allergen implicated in a case of IgE\u2010mediated anaphylaxis to frog muscle. It also shows that a protein belonging to the parvalbumin family is implicated in type I allergies outside the fish species.<\/jats:p>","DOI":"10.1034\/j.1398-9995.2002.23677.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:48:19Z","timestamp":1047347299000},"page":"1053-1058","source":"Crossref","is-referenced-by-count":45,"title":["Severe IgE\u2010mediated anaphylaxis following consumption of fried frog legs: definition of \u03b1\u2010parvalbumin as the allergen in cause"],"prefix":"10.1111","volume":"57","author":[{"given":"C.","family":"Hilger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Grigioni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Thill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Mertens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Hentges","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,10,2]]},"reference":[{"key":"e_1_2_22_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2001.00002.x-i1"},{"key":"e_1_2_22_3_2","first-page":"1676","article-title":"Une cause rare d'asthme allergique professionnel","volume":"61","author":"Charpin J.","year":"1953","journal-title":"Presse Med"},{"key":"e_1_2_22_4_2","first-page":"392","article-title":"A new occupational allergy due to frogs","volume":"51","author":"Nakazawa T","year":"1983","journal-title":"Ann Allergy"},{"key":"e_1_2_22_5_2","first-page":"209","article-title":"Occupational asthma due to frogs","volume":"60","author":"Armentia A","year":"1988","journal-title":"Ann Allergy"},{"key":"e_1_2_22_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000236462"},{"key":"e_1_2_22_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1996.tb00036.x"},{"key":"e_1_2_22_8_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2000.00659.x"},{"key":"e_1_2_22_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb19773.x"},{"key":"e_1_2_22_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(88)90212-6"},{"key":"e_1_2_22_11_2","doi-asserted-by":"publisher","DOI":"10.2165\/00002018-199512050-00002"},{"key":"e_1_2_22_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198306233082513"},{"key":"e_1_2_22_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1998.tb03855.x"},{"key":"e_1_2_22_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0894-7317(99)70110-0"},{"key":"e_1_2_22_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)44043-X"},{"key":"e_1_2_22_16_2","doi-asserted-by":"crossref","first-page":"4332","DOI":"10.1016\/S0021-9258(19)42523-4","article-title":"Isolation and characterization of parvalbumins from skeletal muscle of higher vertebrates","volume":"249","author":"Lehky P","year":"1974","journal-title":"J Biol Chem"},{"key":"e_1_2_22_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00712090"},{"key":"e_1_2_22_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1974.tb01303.x"},{"key":"e_1_2_22_19_2","first-page":"283","article-title":"Characterization of a major allergen (cod). 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