{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,25]],"date-time":"2025-09-25T15:52:20Z","timestamp":1758815540247},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2002,12,11]],"date-time":"2002-12-11T00:00:00Z","timestamp":1039564800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Allergy"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Eotaxin and interleukin\u20105 together provide the signal essential for eosinophil transmigration to airway tissue in allergic reactions. However, it is not known whether peripheral blood eosinophils (PBE) possess an increased transmigration capacity <jats:italic>in vitro<\/jats:italic> after allergen challenge <jats:italic>in vivo<\/jats:italic> before they leave the circulation. We aimed to determine whether PBE in cat\u2010sensitized children have increased spontaneous and\/or eotaxin\u2010induced transmigration capacity <jats:italic>in vitro<\/jats:italic>, and to what extent allergen challenge alters this feature.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> Fourteen cat\u2010allergic children and four healthy controls underwent nasal challenge with cat\u2010allergen. Blood samples were drawn prechallenge and at 2\u2003h and 24\u2003h postchallenge. We analyzed the <jats:italic>in vitro<\/jats:italic> transmigration of PBE, with and without eotaxin as a chemoattractant. We used a transmigration assay with fibronectin\u2010coated membranes. Eosinophil cationic protein (ECP) and PBE counts were run in parallel.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The spontaneous transmigration capacity of eosinophils <jats:italic>in vitro<\/jats:italic> was significantly higher at 2\u2003h after allergen challenge (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.01 <jats:italic>vs.<\/jats:italic> prechallenge) and returned to prechallenge levels at 24\u2003h postchallenge (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.02 <jats:italic>vs.<\/jats:italic> 2\u2003h postchallenge). Addition of eotaxin further augmented the increased transmigration. In concordance, no accompanying changes were measured in the levels of eosinophils in blood or ECP in serum. Furthermore no spontaneous or eotaxin\u2010induced eosinophil transmigration was detected in healthy controls.<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> PBE possess increased spontaneous (and eotaxin\u2010induced) capacity to transmigrate as early as 2\u2003h after allergen challenge in allergic children, without accompanying signs of eosinophil activation in terms of increased PBE count or ECP level. This is probably due to the increased stage of activation of the eosinophil, often referred to as \u201cpriming\u201d.<\/jats:p>","DOI":"10.1034\/j.1398-9995.2002.23714.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T01:48:29Z","timestamp":1047347309000},"page":"1200-1204","source":"Crossref","is-referenced-by-count":7,"title":["Increased eosinophil transmigration after nasal allergen challenge in children with allergic asthma and rhinitis"],"prefix":"10.1111","volume":"57","author":[{"given":"K.","family":"L\u00f6nnkvist","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Moshfegh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Pedroletti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Hedlin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G.","family":"Halld\u00e9n","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Lundahl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,12,11]]},"reference":[{"key":"e_1_2_20_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199010113231505"},{"key":"e_1_2_20_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830271252"},{"key":"e_1_2_20_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1998.028s5071.x"},{"key":"e_1_2_20_5_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm\/136.2.379"},{"key":"e_1_2_20_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1993.tb04705.x"},{"key":"e_1_2_20_7_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.114246"},{"key":"e_1_2_20_8_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.160.5.9812126"},{"key":"e_1_2_20_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70481-1"},{"key":"e_1_2_20_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(97)70158-1"},{"key":"e_1_2_20_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.7.3847"},{"key":"e_1_2_20_12_2","doi-asserted-by":"crossref","first-page":"4593","DOI":"10.4049\/jimmunol.159.9.4593","article-title":"Increased expression of eotaxin in bronchoalveolar lavage and airways of asthmatics contributes to the chemotaxis of eosinophils to the site of inflammation","volume":"159","author":"Lamkhioud B","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_20_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.54.9.825"},{"key":"e_1_2_20_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119234"},{"key":"e_1_2_20_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00390.x"},{"key":"e_1_2_20_16_2","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.4049\/jimmunol.147.8.2595","article-title":"Activation of guinea pig eosinophils by human recombinant IL\u20105. 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