{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T20:49:05Z","timestamp":1792356545409,"version":"4.4.0"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2008,10,9]],"date-time":"2008-10-09T00:00:00Z","timestamp":1223510400000},"content-version":"vor","delay-in-days":3173,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatric Transplantation"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:p>In bone\u2010marrow\u2010transplanted children, early detection of graft failure, relapse, and other potentially treatable problems is facilitated by the use of polymerase chain reaction (PCR) assays that monitor whether blood and marrow cells are of recipient or donor origin. Presence of mixed donor\u2013recipient chimerism (MC) within the first\u2003year after BMT frequently correlates with clinical problems. To study if MC detected one\u2003year or more post\u2010BMT was also often associated with clinical problems, the chimeric status in 33 children surviving 1\u201311\u2003yr (median: 2\u2003yr) after BMT was investigated. A PCR with a sensitivity of 1\u20132%, using fluorescent primers analyzing DNA fragment length polymorphisms, was applied. T\u2010 and B\u2010cells and granulocytes were immunomagnetically isolated and tested separately for all patients. Of the 33 patients, of whom 21 had received pretreatment including total body irradiation (TBI), 27 (82%) exhibited full donor chimerism. Six children (18%), four of whom had received pretreatment without TBI, had MC. In three of these children, all with aplastic anemia, isolated T\u2010cell MC had not posed apparent clinical problems. In two patients, both with MC including B\u2010cells, immune hemolytic anemia was observed. A sixth patient with AML presented with MC and relapse. In two of the six children MC was detected only by cell subset analysis. In conclusion, analysis of MC in leukocyte subsets is more informative than analysis of whole blood only and may reveal clinically important variations in the origin of different cell populations. The prevalence of MC is lower after the first\u2003year post\u2010BMT, and when present is less often associated with clinical problems.<\/jats:p>","DOI":"10.1034\/j.1399-3046.2000.00104.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T18:56:48Z","timestamp":1047495408000},"page":"132-139","source":"Crossref","is-referenced-by-count":16,"title":["Follow\u2010up of chimerism, including T\u2010 andB\u2010lymphocytes and granulocytes in children more than one year after allogeneic bone marrow transplantation"],"prefix":"10.1034","volume":"4","author":[{"given":"Jacek","family":"Winiarski","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c5sa","family":"Gustafsson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Wester","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina","family":"Dalianis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,10,9]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199404283301703"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.1996.tb14026.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001622-199703000-00006"},{"key":"e_1_2_6_5_2","first-page":"91","article-title":"Perspective. 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