{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T04:49:39Z","timestamp":1771476579810,"version":"3.50.1"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,8,29]],"date-time":"2002-08-29T00:00:00Z","timestamp":1030579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Xenotransplantation"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:p>Naturally occurring anti\u2010Gal\u03b11\u20103Gal (anti\u2010Gal) antibodies and complement induce hyperacute rejection (HAR) of porcine organs transplanted to primates. If the hyperacute reaction is prevented, an acute vascular rejection (AVR) occurs within hours to few days. Antibodies are important for the development of AVR, whereas the role of complement is still not clarified. AVR is characterized by protein synthesis\u2010dependent endothelial cell (EC) activation. In the present study we investigated the relation between EC activation as measured by E\u2010selectin expression, and the concentrations of anti\u2010Gal antibodies of IgM, IgG and IgG subclasses in sera from 80 healthy blood donors selected on the basis of sex and age. There was a significant correlation between E\u2010selectin expression and the concentration of IgG3 anti\u2010Gal (<jats:italic>r<\/jats:italic>=0.39; <jats:italic>P<\/jats:italic>=0.019), which was not seen for the other IgG subclasses or for total IgG anti\u2010Gal. A modest, but significant correlation was found between the concentration of IgM anti\u2010Gal and E\u2010selectin expression (<jats:italic>r<\/jats:italic>=0.38; <jats:italic>P<\/jats:italic>=0.040), but not between IgM and IgG3 anti\u2010Gal. There was a large interindividual variation in anti\u2010Gal antibodies, 50\u2010fold for IgM and 70\u2010fold for IgG. Females had significantly higher concentrations of IgM anti\u2010Gal than males (<jats:italic>P<\/jats:italic>=0.0006), which was explained by a substantial increase in IgM anti\u2010Gal concentration in younger women. The concentration of IgG anti\u2010Gal and the degree of E\u2010selectin expression did not differ between sex or age groups. In conclusion, the close correlation between anti\u2010Gal antibodies of the potent complement activating IgG3 subclass and porcine EC activation, may imply that these antibodies play a role in EC activation characteristic of AVR.<\/jats:p>","DOI":"10.1034\/j.1399-3089.2002.01094.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T23:56:32Z","timestamp":1047513392000},"page":"350-358","source":"Crossref","is-referenced-by-count":8,"title":["Human serum\u2010induced porcine endothelial cell E\u2010selectin expression is associated with IgG3 and IgM anti\u2010Gal antibodies"],"prefix":"10.1111","volume":"9","author":[{"given":"Marit","family":"S\u00e6thre","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Una 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