{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:44:23Z","timestamp":1789692263080,"version":"4.0.1"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,6,20]],"date-time":"2002-06-20T00:00:00Z","timestamp":1024531200000},"content-version":"vor","delay-in-days":111,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["APMIS"],"published-print":{"date-parts":[[2002,3]]},"abstract":"<jats:p>Flow cytometry was used to study the expression of leukocyte adhesion molecules CD11a, CD11b, CD11c, CD14, and CD62L (L\u2010selectin) and production of reactive oxygen species (ROS) in an ex vivo human whole\u2010blood system stimulated with lipopolysaccharide\u2010containing outer membrane vesicles (LPS\u2010OMV) from <jats:italic>N. meningitidis.<\/jats:italic> Results demonstrated a dose\u2010dependent increase in surface expression of CD11a, CD11b, CD11c and CD14 in granulocytes and monocytes (maximal at 30\u2013120 min) upon OMV\u2010LPS challenge, whereas CD62L expression was heavily downregulated (maximal at 30\u2013120 min). The OMV\u2010associated LPS was almost as potent (on a weight basis) as purified LPS from <jats:italic>E. coli<\/jats:italic> in inducing adhesion molecule modulation but the response was delayed. Upon stimulation with OMV\u2010LPS or <jats:italic>E. coli<\/jats:italic>\u2010LPS, the production of intracellular ROS increased in both granulocytes and monocytes when dihydroethidium (DHE, mainly reflecting superoxide anion) was used as a probe, whereas peroxynitrite production monitored with dihydrorhodamine 123 (DHR) was not significantly changed. The OMV\u2010mediated modulation of leukocyte adhesion molecule expression and increased ROS production may certainly lead to increased entrapment of leukocytes in the microcirculation and contribute to untoward inflammatory reactions as seen in systemic meningococcal disease.<\/jats:p>","DOI":"10.1034\/j.1600-0463.2002.100301.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T03:25:28Z","timestamp":1047353128000},"page":"193-204","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Outer membrane vesicles from <i>Neisseria meningitidis<\/i>"],"prefix":"10.1111","volume":"110","author":[{"given":"Mohammad Reza","family":"Mirlashari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernst Arne","family":"H\u00f8iby","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johan","family":"Holst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torstein","family":"Lyberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,6,20]]},"reference":[{"key":"e_1_2_9_2_2","first-page":"57","article-title":"The epidemiology of meningococcal disease in Norway 1975\u201391","volume":"14","author":"Lystad A","year":"1991","journal-title":"NIPH Ann"},{"key":"e_1_2_9_3_2","first-page":"1","article-title":"Pathophysiology and management of meningococcal septicaemia","volume":"11","author":"Heyderman RS","year":"1992","journal-title":"Recent Adv Paediatr"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/126.5.514"},{"key":"e_1_2_9_5_2","first-page":"67","article-title":"Production, characterization and control of MenB\u2010vaccine ``Folkehelsa'': an outer membrane vesicle vaccine against group B meningococcal disease","volume":"14","author":"Fredriksen JH","year":"1991","journal-title":"NIPH Ann"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)91961-S"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-1157(89)90017-6"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/346425a0"},{"key":"e_1_2_9_9_2","doi-asserted-by":"crossref","first-page":"532","DOI":"10.4049\/jimmunol.144.2.532","article-title":"Expression of the human leukocyte adhesion molecules, LAM1. 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