{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T10:38:02Z","timestamp":1778755082075,"version":"3.51.4"},"reference-count":64,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2002,1,12]],"date-time":"2002-01-12T00:00:00Z","timestamp":1010793600000},"content-version":"vor","delay-in-days":164,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:p><jats:bold>Introduction:<\/jats:bold> Alterations in the length of DNA repetitive sequences (microsatellite instability (MSI)) represent distinct tumorigenic pathways associated with several familial and sporadic tumors.<\/jats:p><jats:p><jats:bold>Material and methods:<\/jats:bold> To investigate the prevalence and frequency of MSI in melanocytic lesions, the polymerase chain reaction (PCR)\u2010based microsatellite assay was used to examine formalin\u2010fixed, paraffin\u2010embedded tissues of 30 benign melanocytic nevi, 60 melanocytic dysplastic nevi (MDN), and 22 primary vertical growth phase cutaneous malignant melanomas (CMM). Twenty\u2010four microsatellite markers at the 1p, 2p, 3p, 4q and 9p chromosomal regions were used.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> MSI was found at 1p and 9p in MDN and CMM but not in benign melanocytic nevi. The overall prevalence of MSI was17\/60 (28%) in MDN and 7\/22 (31%) in CMM. The frequency of MSI ranged from 2\/24 (9%) to 4\/24 (17%) and was most commonly found at D9S162. There was a statistically significant correlation between degree of atypia and frequency of MSI (p&lt;0.001) in MDN. There were two MSI banding patterns: band shifts and additional bands.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> The data presented revealed the presence of low\u2010frequency MSI (MSI\u2010L) at the 1p and 9p regions in both MDN and CMM. Whether the MSI\u2010L pattern reflects a defect in mismatch repair genes is still to be determined.<\/jats:p>","DOI":"10.1034\/j.1600-0560.2001.280702.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T13:54:05Z","timestamp":1047390845000},"page":"343-350","source":"Crossref","is-referenced-by-count":64,"title":["Comprehensive analysis of 112 melanocytic skin lesions demonstrates microsatellite instability in melanomas and dysplastic nevi, but not in benign nevi"],"prefix":"10.1111","volume":"28","author":[{"given":"Mahmoud R.","family":"Hussein","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralph J.","family":"Tuthill","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eduardo","family":"Roggero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jorge A.","family":"Monti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eulalia C.","family":"Sudilovsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary S.","family":"Wood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oscar","family":"Sudilovsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,1,12]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.47.1.5"},{"key":"e_1_2_6_3_2","doi-asserted-by":"crossref","first-page":"2097","DOI":"10.1001\/jama.1989.03430150065027","article-title":"The emerging epidemic of melanoma and squamous cell skin cancer [see comments]","volume":"262","author":"Glass AG","year":"1989","journal-title":"JAMA"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-7125(16)30966-X"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1978.01640170032006"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19880115)61:2<358::AID-CNCR2820610227>3.0.CO;2-#"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(84)80310-X"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(89)70253-X"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8484121"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.4.12.2622"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/18.1.1"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60100-1"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-230X(08)60824-6"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0696-630"},{"key":"e_1_2_6_15_2","first-page":"675","article-title":"Cancer of the microsatellite mutator phenotype","volume":"377","author":"Perucho M","year":"1996","journal-title":"Biol Chem"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870120208"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(96)00198-0"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12664612"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12276783"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(20000415)86:2<255::AID-IJC16>3.0.CO;2-L"},{"key":"e_1_2_6_21_2","first-page":"5248","article-title":"A National Cancer Institute Workshop on Microsatellite Instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer","volume":"58","author":"Boland CR","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90546-3"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/371075a0"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/5.Supplement_1.1489"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ge.25.120191.001305"},{"key":"e_1_2_6_26_2","first-page":"1713","article-title":"Microsatellite instability in colorectal cancer: different mutator phenotypes and the principal involvement of hMLH1","volume":"58","author":"Thibodeau SN","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_6_27_2","first-page":"1547","article-title":"Instability of microsatellites in human gliomas","volume":"55","author":"Dams E","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90330-S"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8128251"},{"key":"e_1_2_6_30_2","first-page":"306","article-title":"Dysplastic melanocytic nevi: a reproducible histologic definition emphasizing cellular morphology [published erratum appears in Mod Pathol 2: 426][see comments]","volume":"2","author":"Rhodes AR","year":"1989","journal-title":"Mod Pathol"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(99)90192-2"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.2144\/96205st04"},{"key":"e_1_2_6_33_2","first-page":"4739","article-title":"Microsatellite instability analysis: a multicenter study for reliability and quality control","volume":"57","author":"Bocker T","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(98)90430-0"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/17.9.1799"},{"key":"e_1_2_6_36_2","first-page":"172","article-title":"Mutational and expression analysis of the p73 gene in melanoma cell lines","volume":"59","author":"Tsao H","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_6_37_2","first-page":"4841","article-title":"Microsatellite instability in ulcerative colitis\u2010associated colorectal dysplasias and cancers","volume":"54","author":"Suzuki H","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_6_38_2","first-page":"2067","article-title":"Microsatellite instability in preinvasive and invasive head and neck squamous carcinoma","volume":"148","author":"El\u2010Naggar AK","year":"1996","journal-title":"Am J Pathol"},{"key":"e_1_2_6_39_2","first-page":"4483","article-title":"Allelotype mapping of unstable microsatellites establishes direct lineage continuity between endometrial precancers and cancer","volume":"56","author":"Mutter GL","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(97)00052-3"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12323809"},{"key":"e_1_2_6_42_2","first-page":"489","article-title":"Linkage of cutaneous malignant melanoma\/dysplastic nevi to chromosome 9p, and evidence for genetic heterogeneity","volume":"54","author":"Goldstein AM","year":"1994","journal-title":"Am J Hum Genet"},{"key":"e_1_2_6_43_2","first-page":"4749","article-title":"Diagnostic microsatellite instability: definition and correlation with mismatch repair protein expression","volume":"57","author":"Dietmaier W","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_6_44_2","first-page":"2098","article-title":"Frequent microsatellite instability in primary small cell lung cancer","volume":"54","author":"Merlo A","year":"1994","journal-title":"Cancer Res"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0294-152"},{"key":"e_1_2_6_46_2","first-page":"5620","article-title":"Microsatellite instability in bladder cancer","volume":"53","author":"Gonzalez\u2010Zulueta M","year":"1993","journal-title":"Cancer Res"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910640108"},{"key":"e_1_2_6_48_2","first-page":"4021","article-title":"Microsatellite instability correlates with reduced survival and poor disease prognosis in breast cancer","volume":"56","author":"Paulson TG","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_49_2","first-page":"1749","article-title":"Prognostic value and clinicopathological profile of microsatellite instability in gastric cancer","volume":"4","author":"Wirtz HC","year":"1998","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_50_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.42.5.673"},{"key":"e_1_2_6_51_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202413"},{"key":"e_1_2_6_52_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v111.pm8690195"},{"key":"e_1_2_6_53_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.52.6.455"},{"key":"e_1_2_6_54_2","first-page":"4662","article-title":"Microsatellite instability\u2010associated mutations associate preferentially with the intestinal type of primary gastric carcinomas in a high\u2010risk population","volume":"56","author":"Chung YJ","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_55_2","first-page":"5545","article-title":"A transforming growth factor beta receptor type II gene mutation common in colon and gastric but rare in endometrial cancers with microsatellite instability","volume":"55","author":"Myeroff LL","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_6_56_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1201816"},{"key":"e_1_2_6_57_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65665-9"},{"key":"e_1_2_6_58_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/BF00281607","article-title":"The yeast gene MSH3 defines a new class of eukaryotic MutS homologues","volume":"239","author":"New L","year":"1993","journal-title":"Mol Gen Genet"},{"key":"e_1_2_6_59_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7604264"},{"key":"e_1_2_6_60_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7604266"},{"key":"e_1_2_6_61_2","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.2870140305"},{"key":"e_1_2_6_62_2","first-page":"2225","article-title":"HMSH6 alterations in patients with microsatellite instability\u2010low colorectal cancer [in process citation]","volume":"60","author":"Parc YR","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_6_63_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7604265"},{"key":"e_1_2_6_64_2","first-page":"2733","article-title":"Detection of a mutator phenotype in cancer cells by inter\u2010Alu polymerase chain reaction","volume":"56","author":"Krajinovic M","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(98)90221-0"}],"container-title":["Journal of Cutaneous Pathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0560.2001.280702.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0560.2001.280702.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0560.2001.280702.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T21:57:12Z","timestamp":1697493432000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0560.2001.280702.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,8]]},"references-count":64,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2001,8]]}},"alternative-id":["10.1034\/j.1600-0560.2001.280702.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0560.2001.280702.x","archive":["Portico"],"relation":{},"ISSN":["0303-6987","1600-0560"],"issn-type":[{"value":"0303-6987","type":"print"},{"value":"1600-0560","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,8]]}}}