{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:43:33Z","timestamp":1789382613223,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2002,1,12]],"date-time":"2002-01-12T00:00:00Z","timestamp":1010793600000},"content-version":"vor","delay-in-days":72,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2001,11]]},"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> An association between <jats:italic>Borrelia burgdorferi<\/jats:italic> and cutaneous B\u2010cell lymphoma (CBCL) has been made in several European countries. The evidence in favor of such an association has recently been based on more definitive tests for the pathogenetic role of <jats:italic>B. burgdorferi<\/jats:italic> in CBCL, including positive cultures or polymerase chain reaction (PCR) amplification of borrelial DNA from lesional skin. However, there is only one report of <jats:italic>B. burgdorferi<\/jats:italic> in four North American cases of B\u2010cell lymphoma.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> We retrieved 38 cases of primary and secondary CBCL from different geographic regions of the United States. Two separate techniques were used to detect borrelial DNA by PCR, a nested PCR method to amplify a <jats:italic>B. burgdorferi<\/jats:italic>\u2010specific gene as well as a borrelial chromosomal Ly\u20101 clone amplification method. Southern blot hybridization was used for confirmation of the PCR results.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> No <jats:italic>B. burgdorferi<\/jats:italic>\u2010specific DNA was detected in any of the 38 CBCL cases, whereas detectable PCR products were obtained with our positive controls.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> Our findings, in light of previous studies, suggest that <jats:italic>B. burgdorferi<\/jats:italic> plays a minimal role in the development or pathogenesis of CBCL in the United States. The findings also suggest that the geographic variations in the clinical manifestations of <jats:italic>B. burgdorferi<\/jats:italic> are indeed real and may be secondary to the genetic and phenotypic differences between <jats:italic>B. burgdorferi<\/jats:italic> strains present in Europe and North America.<\/jats:p>","DOI":"10.1034\/j.1600-0560.2001.281002.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T13:52:41Z","timestamp":1047390761000},"page":"502-507","source":"Crossref","is-referenced-by-count":135,"title":["Absence of <i>Borrelia burgdorferi<\/i> DNA in cutaneous B\u2010cell lymphomas from the United States"],"prefix":"10.1111","volume":"28","author":[{"given":"Gary S.","family":"Wood","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nandan 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