{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:04:07Z","timestamp":1765728247045},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,7,18]],"date-time":"2002-07-18T00:00:00Z","timestamp":1026950400000},"content-version":"vor","delay-in-days":78,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cutan Pathol"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:p> <jats:bold>Background:<\/jats:bold> HHV\u20108 has been identified as the causative agent of Kaposi's sarcoma (KS) and some lymphoproliferative disorders. In addition, there are anecdotal reports on the presence of HHV\u20108 in other tumors, especially cutaneous epithelial and mesenchymal neoplasms. The aim of the study was to ascertain the value of identification of HHV\u20108 viral DNA sequences in routinely processed, formalin\u2010fixed, paraffin\u2010embedded tissues for the diagnosis of Kaposi's sarcoma and other mesenchymal tumors.<\/jats:p><jats:p> <jats:bold>Methods:<\/jats:bold> The presence of HHV\u20108 sequences in archival material was studied by nested PCR using specific primers for amplification of a 233\u2010bp long fragment of HHV\u20108 (ORF 26).<\/jats:p><jats:p> <jats:bold>Results:<\/jats:bold> Thirty\u2010three patients with KS (18 classic\/sporadic, six post\u2010transplant and nine AIDS\u2010related) and various mesenchymal tumors and related conditions (n\u2003=\u200376) were studied. HHV\u20108 DNA sequences were detected in 29 of the 33 cases of KS and in one case of multiple eruptive dermatofibroma (MEDF).<\/jats:p><jats:p> <jats:bold>Conclusions:<\/jats:bold> Identification of HHV\u20108 DNA sequences in routinely processed tissue is a useful diagnostic marker for KS. Although other mesenchymal tumors are usually not associated with HHV\u20108, its presence is not fully specific for KS since HHV\u20108 sequences were also found in one case of MEDF. Therefore, PCR analysis for the detection of HHV\u20108 should only be used as an additional diagnostic marker for KS and in the context of other tools such as routine histology.<\/jats:p>","DOI":"10.1034\/j.1600-0560.2002.290503.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T13:55:18Z","timestamp":1047390918000},"page":"279-281","source":"Crossref","is-referenced-by-count":16,"title":["Study of HHV\u20108 DNA sequences in archival biopsies from lesional skin of Kaposi's sarcoma, various mesenchymal tumors and related reactive conditions"],"prefix":"10.1111","volume":"29","author":[{"given":"Dmitry V.","family":"Kazakov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bettina 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