{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:19:59Z","timestamp":1791332399334,"version":"4.3.1"},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":603,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:p>Abstract: Stromal cell\u2010derived factor\u20101 (SDF\u20101) is a chemokine produced by bone marrow stromal cells which plays an important role in B\u2010lymphopoiesis and the homing of hematopoietic stem cells to the bone marrow. In the present study, we investigated the role of SDF\u20101 and its receptor, CXCR4, in the chemotactic interaction between non\u2010Hodgkin B\u2010lymphoma cells and lymph node stromal cells. SDF\u20101 mRNA was abundantly expressed in stromal cells isolated from the lymph nodes of patients with malignant lymphoma. All B\u2010lymphoma cells freshly isolated from these patients and most laboratory B\u2010lymphoma cell lines, including follicular, diffuse large, and Burkitt's lymphoma cells, expressed surface CXCR4 and migrated in the presence of recombinant human SDF\u20101\u03b1. Chemotaxis assays revealed that CXCR4\u2010positive (but not CXCR4\u2010negative) B\u2010lymphoma cells migrated towards lymph node stromal cells, and this migration was almost completely inhibited by the addition of anti\u2010CXCR4 monoclonal antibody to the lymphoma cells or of anti\u2010SDF\u20101 neutralizing antibody to the culture supernatant of the stromal cells. Down\u2010regulation of surface CXCR4 was detected in B\u2010lymphoma cells which migrated towards the stromal cells but not in those which showed no migratory response. In addition, contact between the lymphoma cells and the stromal cells resulted in down\u2010regulation of surface CXCR4 on the lymphoma cells. These data strongly suggest that SDF\u20101\/CXCR4 is the main chemokine system involved in the chemotactic interaction between B\u2010lymphoma cells and lymph node stromal cells.<\/jats:p>","DOI":"10.1034\/j.1600-0609.2000.90147.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T18:48:44Z","timestamp":1047408524000},"page":"323-332","source":"Crossref","is-referenced-by-count":67,"title":["Stromal cells in lymph nodes attractB\u2010lymphoma cells via production ofstromal cell\u2010derived factor\u20101"],"prefix":"10.1111","volume":"64","author":[{"given":"Junko","family":"Arai","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Yasukawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Yakushijin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuhiko","family":"Miyazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0609.2000.90147.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0609.2000.90147.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0609.2000.90147.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T17:59:47Z","timestamp":1698602387000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0609.2000.90147.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,5]]}},"alternative-id":["10.1034\/j.1600-0609.2000.90147.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0609.2000.90147.x","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,5]]}}}