{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T23:50:12Z","timestamp":1785801012354,"version":"3.56.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":480,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2000,9]]},"abstract":"<jats:p>Abstract: The Philadelphia (Ph) chromosome is observed in approximately 1% of patients with acute myeloblastic leukaemia (AML), especially subtypes M1 and M2 in the French\u2013American\u2013British classification. We describe here a cytogenetic and molecular investigation of a rare case with Ph\u2010positive AML M6 (erythroleukaemia). A 63\u2010yr\u2010old woman was diagnosed as having erythroleukaemia. Leukaemic cells were positive for CD4 and CD7 as well as CD13, CD33, CD34 and HLA\u2010DR. They were analyzed by G\u2010banding, fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH), Southern blot and reverse transcriptase polymerase chain reaction analyses. The karyotypes at diagnosis were as follows: 61, XX, \u2212X, \u22121, \u22122, \u22123, \u22124, \u22125, \u22127, t(9;22)(q34;q11)\u00d72, \u221215, \u221216, \u221217, \u221218, +19, +21, +22 [3]\/61, idem, \u221222, +der(22)t(9;22) [36]. FISH with <jats:italic>BCR\/ABL <\/jats:italic>probes showed that 39% and 57% of interphase nuclei had double and triple <jats:italic>BCR\/ABL<\/jats:italic> fusion signals, respectively. Chromosome analysis in complete remission showed a normal karyotype in all 20 metaphases, confirming the diagnosis as Ph positive\u2010acute leukaemia. FISH at relapse showed that 92% of interphase nuclei had triple fusion signals. Rearrangement of major breakpoint cluster region (M\u2010<jats:italic>bcr<\/jats:italic>) in the <jats:italic>BCR<\/jats:italic> gene and coexpression of p210\u2010type (b2a2) and p190\u2010type (e1a2) <jats:italic>BCR\/ABL<\/jats:italic> fusion transcripts due to alternative splicing were also detected. We conclude that clonal evolution from double to triple Ph chromosomes may be implicated in the disease progression. Considering other two reported cases, Ph\u2010positive erythro\u2010leukaemia appears to be correlated with coexpression of myeloid\/T\u2010lymphoid markers and hyperdiploidy with double or triple Ph chromosomes, although breakpoints in the <jats:italic>BCR<\/jats:italic> gene are heterogenous.<\/jats:p>","DOI":"10.1034\/j.1600-0609.2000.90253.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T18:50:11Z","timestamp":1047408611000},"page":"182-187","source":"Crossref","is-referenced-by-count":7,"title":["Triple Philadelphia chromosomes with major\u2010<i>bcr<\/i> rearrangement in hypotriploid erythroleukaemia"],"prefix":"10.1111","volume":"65","author":[{"given":"Katsuya","family":"Yamamoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichi","family":"Nakamura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Honoka","family":"Arai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Saito","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinpei","family":"Furusawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"container-title":["European Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0609.2000.90253.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0609.2000.90253.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0609.2000.90253.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T07:50:08Z","timestamp":1698565808000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0609.2000.90253.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,9]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,9]]}},"alternative-id":["10.1034\/j.1600-0609.2000.90253.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0609.2000.90253.x","archive":["Portico"],"relation":{},"ISSN":["0902-4441","1600-0609"],"issn-type":[{"value":"0902-4441","type":"print"},{"value":"1600-0609","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,9]]}}}