{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,18]],"date-time":"2023-10-18T06:50:39Z","timestamp":1697611839544},"reference-count":16,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,10,4]],"date-time":"2002-10-04T00:00:00Z","timestamp":1033689600000},"content-version":"vor","delay-in-days":64,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["European J of Haematology"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:p><jats:bold>Abstract:<\/jats:bold> A 64\u2010yr\u2010old Japanese man presented with mild anemia, leukocytosis, and thrombocytosis in November 1999. A diagnosis of chronic myeloid leukemia was made with a positive Ph chromosome, and interferon \u03b1 treatment was started, 6 million units a day. Two years later, in October 2001, the patient developed leukocytosis, an increased LDH level, and large blasts with basophilic cytoplasm with cytoplasmic projections appeared in the peripheral blood. Bone marrow aspiration revealed increased blasts (59.6%). These blasts were negative on peroxidase stain, positive on acid phosphatase, and weakly positive on \u03b1 naphthyl butyrate esterase stain and periodic acid\u2010Schiff stain. Immunohistochemical staining with monoclonal antibodies revealed that these blasts were strongly positive with anti\u2010CD41 (glycoprotein IIb\/IIIa), weakly positive with CD7, CD33, and CD34, and negative with other monoclonal antibodies. A diagnosis of megakaryoblastic transformation from chronic myeloid leukemia was therefore made. Two\u2010color fluorescence <jats:italic>in situ<\/jats:italic> hybridization (FISH) for portions of the major\u2010bcr and abl genes from bone marrow cells revealed two fused signals in 90.6% and one fused signal in 5.8% of 106 cells. A cytogenetic study revealed that bone marrow cells were 69, XYY, +6, \u22127, +8, \u22129, t(9;22)(q34;q11), +11, +13, \u221215, \u221216, dic(17;18)(p11;p11), \u221218, +19, +21, der(22)t(9;22) in six of nine examined cells. These findings confirmed that these megakaryoblasts originated from megakaryocytes of the chronic myeloid leukemia clone.<\/jats:p>","DOI":"10.1034\/j.1600-0609.2002.02731.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T18:53:28Z","timestamp":1047408808000},"page":"122-125","source":"Crossref","is-referenced-by-count":3,"title":["Double Ph\u2010positive megakaryoblastic transformation of chronic myeloid leukemia"],"prefix":"10.1111","volume":"69","author":[{"given":"Yuko","family":"Hirose","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasufumi","family":"Masaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kawabata","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriyoshi","family":"Ogawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuji","family":"Wano","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susumu","family":"Sugai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,10,4]]},"reference":[{"key":"e_1_2_10_2_2","first-page":"85","article-title":"Differentiation patterns in the blastic phase of chronic myeloid leukemia","volume":"61","author":"Griffin JD","year":"1983","journal-title":"Blood"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1985.tb07326.x"},{"key":"e_1_2_10_4_2","first-page":"125","article-title":"Analysis of immunophenotype, genotype, and lineage fidelity in blastic transformation of chronic myelogenous leukemia: a study of 20 cases","volume":"111","author":"Adachi K","year":"1988","journal-title":"J Lab Clin Med"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1991.tb08048.x"},{"key":"e_1_2_10_6_2","first-page":"1349","article-title":"Immunophenotypic characteristics of blast crisis of myeloid leukaemia: Correlations with clinico\u2010biological features and survival","volume":"7","author":"Urbano\u2010Ispizua A","year":"1993","journal-title":"Leukemia"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/10428199509107903"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002770050233"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19820301)49:5<921::AID-CNCR2820490516>3.0.CO;2-W"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00320522"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1987.00331.x"},{"key":"e_1_2_10_12_2","first-page":"1360","article-title":"A differentiation potency of blasts in chronic myelogenous leukemia in blastic crisis to megakaryocytic lineage","volume":"49","author":"Sato T","year":"1986","journal-title":"Acta Haematol Jpn"},{"key":"e_1_2_10_13_2","first-page":"74","article-title":"Chronic myelogenous leukemia with megakaryocytic transformation presenting cranial nerve palsy. 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