{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:55:06Z","timestamp":1791201306006,"version":"4.2.0"},"reference-count":58,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,5,2]],"date-time":"2002-05-02T00:00:00Z","timestamp":1020297600000},"content-version":"vor","delay-in-days":31,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:p><jats:bold>Abstract:<\/jats:bold> External assault to the skin is followed by an epidermal response including synthesis of DNA, lipids, cytokines and migration of antigen presenting cells. MIP\u20103\u03b1 (CCL20, LARC, Exodus\u20101, Scya20) is a recently described C\u2010C chemokine, predominantly expressed in extralymphoid tissue, which is known to direct migration of dendritic cell precursors and memory lymphocytes to sites of antigen invasion. We assessed the expression of MIP\u20103\u03b1 in human skin using semi\u2010quantitative polymerase chain reaction. <jats:italic>In vivo<\/jats:italic>, MIP\u20103\u03b1 mRNA was constitutively expressed at low levels in untreated human epidermis. After acute disruption of the epidermal permeabiltiy barrier MIP\u20103\u03b1 mRNA was upregulated in the epidermal fraction, whereas dermal MIP\u20103\u03b1 mRNA levels remained unchanged. <jats:italic>In vitro<\/jats:italic>, MIP\u20103\u03b1 was increased in cultured keratinocytes treated with IL\u20101\u03b1 and TNF\u2010\u03b1 and was present in immature and mature dendritic cells, THP\u20101 monocytic cells and activated T cells. Finally, skin biopsies from patients with psoriasis, contact dermatitis and mycosis fungoides showed abundant expression. In biopsies from atopic dermatitis and graft vs. host disease a weak signal was present, whereas no expression was found in scleroderma and toxic epidermal necrolysis. We conclude that regulation of MIP\u20103\u03b1 mRNA is part of the epidermal response to external assault. Its upregulation may represent a danger signal for increased immunosurveillance in barrier disrupted skin and inflammatory skin conditions with impaired barrier function to counteract potential antigen invasion.<\/jats:p>","DOI":"10.1034\/j.1600-0625.2002.110205.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T20:27:37Z","timestamp":1047414457000},"page":"135-142","source":"Crossref","is-referenced-by-count":84,"title":["Expression of the C\u2010C chemokine MIP\u20103\u03b1\/CCL20 in human epidermis with impaired permeability barrier 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