{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:04:04Z","timestamp":1765728244976},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,7,8]],"date-time":"2002-07-08T00:00:00Z","timestamp":1026086400000},"content-version":"vor","delay-in-days":37,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Experimental Dermatology"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:p><jats:bold>Abstract:<\/jats:bold> Chronic ulcerative stomatitis protein (CUSP), the most abundant cutaneous isoform of <jats:italic>p63<\/jats:italic>, is a <jats:italic>p53<\/jats:italic>\u2010related gene essential for epithelial development. CUSP lacks the N\u2010terminal transactivation domain found on other p53 family members and has been shown to inhibit p53 function <jats:italic>in vitro<\/jats:italic>. In this study, biopsies of normal skin (21 of 21), benign neoplasms [seborrheic keratosis (3 of 3), acrochordon (2 of 3), and verruca plana (3 of 3)], and squamous cell carcinomas (SCC) (4 of 4) displayed strong nuclear CUSP immuno\u2010reactivity in epidermal cells. In contrast few basal cell carcinomas (BCC) (7 of 27) and sebaceous nevi (1 of 2) displayed this pattern of CUSP immunoreactivity. Thus, biopsies of cutaneous conditions characterized by sonic hedgehog (SHH) pathway dysregulation were more than 86 times as likely to lack CUSP\/p63 immunofluorescence as were other cutaneous samples. Adjacent normal\u2010appearing skin from patients with basal cell nevus syndrome (BCNS) (2 of 3) also lacked CUSP immuno\u2010staining. Lastly, a BCC arising in a <jats:italic>patched<\/jats:italic> heterozygous mouse also lacked CUSP immuno\u2010staining. Because <jats:italic>CUSP<\/jats:italic> mRNA and protein were detected via Northern and Western analysis in BCC samples lacking CUSP immuno\u2010staining, we sequenced the coding region of <jats:italic>CUSP<\/jats:italic> from two non\u2010staining BCCs but found no mutations. Therefore, CUSP appears to be present, unmutated, and yet frequently undetectable by immunofluorescence in cutaneous lesions in both humans and mice that are associated with SHH pathway dysregulation (BCCs, BCNS, and nevus sebaceous).<\/jats:p>","DOI":"10.1034\/j.1600-0625.2002.110302.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T20:27:38Z","timestamp":1047414458000},"page":"203-208","source":"Crossref","is-referenced-by-count":14,"title":["CUSP\/p63 expression in basal cell carcinoma"],"prefix":"10.1111","volume":"11","author":[{"given":"Robert P.","family":"Dellavalle","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Walsh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Marchbank","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy E.","family":"Grayson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lih\u2010Jen","family":"Su","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva R.","family":"Parker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"DeGregori","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristi","family":"Penheiter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle","family":"Aszterbaum","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ervin H.","family":"Epstein jr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lela A.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,7,8]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/356215a0"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/19539"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/19531"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81646-3"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(00)80275-0"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(01)00105-0"},{"key":"e_1_2_7_8_2","first-page":"3370","article-title":"Frequent alteration of p63 expression in human primary bladder carcinomas","volume":"60","author":"Park B J","year":"2000","journal-title":"Can Res"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.2000.6026"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1053\/hupa.2001.24324"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1177\/120347549900300511"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202955"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1905840"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.neo.7900008"},{"key":"e_1_2_7_15_2","first-page":"4165","article-title":"Mutational analysis of the p63\/p73L\/p51\/p40\/CUSP\/KET gene in human cancer cell lines using intronic primers","volume":"59","author":"Hagiwara K","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0798-839"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.1999.00651.x"},{"key":"e_1_2_7_18_2","first-page":"261","article-title":"Is chronic ulcerative stomatitis an entity? Clinical and immunological findings in 18 cases","volume":"8","author":"Chorzelski T","year":"1998","journal-title":"Eur J Dermatol"},{"key":"e_1_2_7_19_2","first-page":"4016","article-title":"Down\u2010regulation of p63 is required for epidermal UV\u2010B\u2010induced apoptosis","volume":"60","author":"Liefer K M","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/15242"},{"key":"e_1_2_7_21_2","first-page":"4165","article-title":"Mutational analysis of the p63\/p73L\/p51\/p40\/CUSP\/KET gene in human cancer cell lines using intronic primers","volume":"59","author":"Hagiwara K","year":"1999","journal-title":"Can Res"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1054\/bjoc.2000.1735"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5268.1668"},{"key":"e_1_2_7_24_2","first-page":"1834","article-title":"The sebaceous nevus. a nevus with deletions of the PTCH gene","volume":"59","author":"Xin H","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_7_25_2","first-page":"4731","article-title":"Patched (ptch)\u2010associated preferential expression of smoothened in human basal cell carcinoma of the skin","volume":"57","author":"Kallassy M","year":"1997","journal-title":"Cancer Res"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.147.1.71"}],"container-title":["Experimental Dermatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0625.2002.110302.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0625.2002.110302.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0625.2002.110302.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T14:02:46Z","timestamp":1697551366000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0625.2002.110302.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6]]},"references-count":25,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["10.1034\/j.1600-0625.2002.110302.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0625.2002.110302.x","archive":["Portico"],"relation":{},"ISSN":["0906-6705","1600-0625"],"issn-type":[{"value":"0906-6705","type":"print"},{"value":"1600-0625","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6]]}}}