{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T15:29:36Z","timestamp":1790177376186,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,11,11]],"date-time":"2002-11-11T00:00:00Z","timestamp":1036972800000},"content-version":"vor","delay-in-days":740,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Pineal Research"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:p>Alloxan can act as a generator of reactive oxygen species (ROS) as long as sufficient suitable reducing agents (e.g. reduced glutathione) and oxygen are available. Using electron spin resonance\u2010spectroscopy and the oxygen\u2010centered spin trap DEPMPO, we demonstrate that hydroxyl radicals (OH&amp;#x007a;rad;) are formed in vitro by alloxan in the presence of glutathione (GSH) and chelated divalent iron. Furthermore, peroxidation of polyunsaturated fatty acids from phosphatidylcholine\u2010containing liposomes with concomitant formation of malondialdehyde (MDA) was used as a further indicator for a preceding OH&amp;#x007a;rad; formation. Melatonin, the main secretory product of the pineal gland, is an effective scavenger of OH&amp;#x007a;rad;. The 50%\u2010inhibitor concentration (IC50\u2010value) for melatonin to scavenge OH&amp;#x007a;rad; generated from the alloxan\/GSH\u2010reaction in the presence of ferrous ions was 23 \u03bcmol\/L. In contrast to the ability to effectively scavenge OH&amp;#x007a;rad;, the potential of melatonin to prevent lipid peroxidation is considerably less pronounced.<\/jats:p>","DOI":"10.1034\/j.1600-0633.2002.290402.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T08:30:58Z","timestamp":1047457858000},"page":"201-208","source":"Crossref","is-referenced-by-count":79,"title":["Scavenging effect of melatonin on hydroxyl radicals generated by alloxan"],"prefix":"10.1111","volume":"29","author":[{"given":"H.J.","family":"Br\u00f6mme","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"M\u00f6rke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Peschke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Ebelt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Peschke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,11,11]]},"container-title":["Journal of Pineal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0633.2002.290402.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0633.2002.290402.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0633.2002.290402.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T05:29:25Z","timestamp":1695878965000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0633.2002.290402.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,11]]}},"alternative-id":["10.1034\/j.1600-0633.2002.290402.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0633.2002.290402.x","archive":["Portico"],"relation":{},"ISSN":["0742-3098","1600-079X"],"issn-type":[{"value":"0742-3098","type":"print"},{"value":"1600-079X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,11]]}}}