{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,30]],"date-time":"2023-10-30T05:09:51Z","timestamp":1698642591467},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":693,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:p>Intracellular pH (pH<jats:sub>i<\/jats:sub>) and several ion transport mechanisms in cultured murine salivary gland cells (SCA\u20109) were studied using a videomicrofluorometric method and the H<jats:sup>+<\/jats:sup>\u2010specific probe C\u2010SNARF\u20101. The aim of this study was to test SCA\u20109 cells' pH<jats:sub>i<\/jats:sub> regulation mechanisms and evaluate if this cell line is representative of submandibular gland cells. Resting pH<jats:sub>i<\/jats:sub> in unstimulated cells was estimated to be 7.17\u00b10.07. To investigate the presence of Na<jats:sup>+<\/jats:sup>\/H<jats:sup>+<\/jats:sup> and Cl<jats:sup>\u2212<\/jats:sup>\/HCO<jats:sub>3<\/jats:sub><jats:sup>\u2212<\/jats:sup> antiports as well as Na<jats:sup>+<\/jats:sup>\/K<jats:sup>+<\/jats:sup>\/2Cl<jats:sup>\u2212<\/jats:sup> symports in SCA\u20109 cells, we used different specific blockers, dimethyl\u2010amiloride, disulfonic stilbene, bumetanide and furosemide. In order to study SCA\u20109 cell capacity to regulate their pH<jats:sub>i<\/jats:sub> in response to alkaline and acid loads, we applied the NH<jats:sub>4<\/jats:sub>Cl prepulse method to all these blockers. The results showed that SCA\u20109 cells possess both antiports and symports involved in pH<jats:sub>i<\/jats:sub> regulation, and that this cell line can be used as a convenient model to study pH<jats:sub>i<\/jats:sub> regulatory mechanisms in salivary cells.<\/jats:p>","DOI":"10.1034\/j.1600-0722.2000.00670.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T19:44:25Z","timestamp":1047411865000},"page":"54-58","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Cultured tumor cells of murine submandibular gland origin: a model to investigate pH<sub>i<\/sub> regulation of salivary cells"],"prefix":"10.1111","volume":"108","author":[{"given":"Estelle","family":"Trzaskawka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean","family":"Vigo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Christophe","family":"Egea","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie\u2010Christine","family":"Goldsmith","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean\u2010Marie","family":"Salmon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dominique","family":"Deville De Periere","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0722.2000.00670.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0722.2000.00670.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0722.2000.00670.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T18:48:07Z","timestamp":1698605287000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0722.2000.00670.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["10.1034\/j.1600-0722.2000.00670.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0722.2000.00670.x","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,2]]},"assertion":[{"value":"2001-12-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}