{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T11:38:36Z","timestamp":1767008316963},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,1,12]],"date-time":"2002-01-12T00:00:00Z","timestamp":1010793600000},"content-version":"vor","delay-in-days":42,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:p>Gingival epithelial cells (GEC) are the first cells of the host that encounter the periodontal pathogens, and therefore their role in the initiation of the inflammatory response is critical. We aimed to: 1) characterize the expression of interleukin (IL)\u20101\u03b1 and IL\u20101\u03b2 in human gingiva and cultured GEC; 2) demonstrate the ability of <jats:italic>A. actinomycetemcomitans<\/jats:italic> extracts to upregulate IL\u20101\u03b1, IL\u20101\u03b2 and IL\u20108 expression in GEC <jats:italic>in vitro;<\/jats:italic> and 3) characterize the role of IL\u20101\u03b1 and IL\u20101\u03b2 in the induction of IL\u20108 expression in GEC <jats:italic>in vitro<\/jats:italic>. Ten gingival biopsies (5 inflamed and 5 controls) and cultured GEC were examined for IL\u20101\u03b1 and IL\u20101\u03b2 using immunohistochemical techniques. GEC were also challenged with <jats:italic>A. actinomycetemcomitans<\/jats:italic> extracts or IL\u20101, and secretion of IL\u20101 and IL\u20108 was determined by ELISA. <jats:italic>In vivo<\/jats:italic>, IL\u20101\u03b1 and IL\u20101\u03b2 were localized in the gingival epithelium and the infiltrating leukocytes. <jats:italic>In vitro, A. actinomycetemcomitans<\/jats:italic> extracts induced a time\u2010dependent expression of IL\u20101\u03b1, IL\u20101\u03b2 and IL\u20108 in GEC. IL\u20101 inhibitors did not affect <jats:italic>A. actinomycetemcomitans<\/jats:italic>\u2010induced IL\u20108, although they inhibited IL\u20108 induced by IL\u20101\u03b1 or IL\u20101\u03b2. In conclusion, GEC are a major source of IL\u20101\u03b1 and IL\u20101\u03b2 in the periodontium, which in turn induce additional inflammatory mediators such as IL\u20108. Therefore GEC can be a potential target for therapeutic intervention in the future.<\/jats:p>","DOI":"10.1034\/j.1600-0722.2001.00162.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T19:46:32Z","timestamp":1047411992000},"page":"393-401","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<i>Actinobacillus actinomycetemcomitans<\/i>\u2010induced expression of IL\u20101\u03b1 and IL\u20101\u03b2 in human gingival epithelial cells: role in IL\u20108 expression"],"prefix":"10.1111","volume":"109","author":[{"given":"Andreas","family":"Sfakianakis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles E.","family":"Barr","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Don 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