{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:09:26Z","timestamp":1762099766434},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,12,21]],"date-time":"2001-12-21T00:00:00Z","timestamp":1008892800000},"content-version":"vor","delay-in-days":264,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2001,4]]},"abstract":"<jats:p>The effects of radiation on the Ca<jats:sup>2+<\/jats:sup> signaling system in HSY cells transfected with the Bcl\u20102 or Bcl\u2010XL gene were studied. Bcl\u20102 overexpression did not alter carbachol (CCh)\u2010elicited initial increase in cytosolic free Ca<jats:sup>2+<\/jats:sup> concentrations ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>), but Bcl\u2010XL overexpression dramatically reduced this response. Exposure to 10\u2003Gy \u03b3\u2010ray did not alter basal [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub>. By contrast, the CCh\u2010stimulated initial [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i <\/jats:sub>increase was reduced at 0.5 and 4\u2003h post\u2010irradiation in all cell types and remained decreased at 24\u2003h in wild\u2010type and control\u2010transfected cells, but recovered in Bcl\u20102\u2010 and Bcl\u2010XL\u2010transfectants. The formation of inositol 1,4,5\u2010trisphosphate (IP<jats:sub>3<\/jats:sub>) in response to CCh at 4\u2010h post\u2010irradiation was decreased in wild\u2010type and control\u2010transfected cells, but not in Bcl\u20102 and Bcl\u2010XL transfectants. The capacity of the IP<jats:sub>3<\/jats:sub>\u2010sensitive Ca<jats:sup>2+<\/jats:sup> store was significantly reduced by radiation in all cells except Bcl\u2010XL transfectants. Ca<jats:sup>2+<\/jats:sup> influx after stimulation with CCh was suppressed by exposure to radiation in wild\u2010type and control\u2010transfected cells, but not in Bcl\u20102\u2010 and Bcl\u2010XL\u2010transfectants. However, radiation enhanced Ca<jats:sup>2+<\/jats:sup> influx activated by thapsigargin in all cell types. These results suggest that 1) radiation diminishes IP<jats:sub>3<\/jats:sub> formation and Ca<jats:sup>2+<\/jats:sup>release in response to CCh, but potentiates the store\u2010operated Ca<jats:sup>2+<\/jats:sup> influx; and 2) overexpression of Bcl\u20102 or Bcl\u2010XL partially protects cells from radiation\u2010induced inhibition of Ca<jats:sup>2+<\/jats:sup> signaling.<\/jats:p>","DOI":"10.1034\/j.1600-0722.2001.00982.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T19:45:44Z","timestamp":1047411944000},"page":"103-108","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Effects of radiation on Ca<sup>2+<\/sup> signaling in salivary epithelial cell lines transfected with Bcl\u20102 and Bcl\u2010XL"],"prefix":"10.1111","volume":"109","author":[{"given":"Xiuhua","family":"Sun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010bing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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