{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T04:12:55Z","timestamp":1793679175302,"version":"4.5.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,6,4]],"date-time":"2003-06-04T00:00:00Z","timestamp":1054684800000},"content-version":"vor","delay-in-days":488,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:p>Epithelial cells appear to play an important role in the initiation and maintenance of autoimmune lesions in the salivary glands of patients with Sj\u00f6gren's syndrome. Therefore, the detailed study of immunological function of salivary gland epithelial cells (SGEC) may provide useful information for the understanding of Sj\u00f6gren's syndrome pathogenesis. In this report we aimed to formulate a protocol for the establishment of human non\u2010neoplastic SGEC lines as a tool for the study of the physiology and pathophysiology of these cells. Pointing towards a practical approach, we sought to establish SGEC lines from quite a limited amount of biopsy tissue obtained during the diagnostic evaluation of patients. Herein, the favorable conditions for the long\u2010term maintenance of human non\u2010neoplastic SGEC lines are presented and involve the successive application of a serum\u2010containing and a serum\u2010free culture medium, supplemented with essential epithelial growth factors. This protocol has been found reliable and convenient, as attested by the reproducible establishment of non\u2010neoplastic SGEC lines. The analysis of SGEC phenotypic features, as well as a coculture system for the study of interactions between epithelial cells and lymphocytes, are also described. Such techniques may provide valuable means for the functional and molecular investigation of human SGEC and particularly for the study of Sj\u00f6gren's syndrome and other disorders of glandular epithelia.<\/jats:p>","DOI":"10.1034\/j.1600-0722.2002.00152.x","type":"journal-article","created":{"date-parts":[[2004,12,23]],"date-time":"2004-12-23T07:59:02Z","timestamp":1103788742000},"page":"21-30","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":77,"title":["Establishment of a convenient system for the long\u2010term culture and study of non\u2010neoplastic human salivary gland epithelial cells"],"prefix":"10.1111","volume":"110","author":[{"given":"Ioannis D.","family":"Dimitriou","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Efstathia K.","family":"Kapsogeorgou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasmi F.","family":"Abu\u2010Helu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haralampos M.","family":"Moutsopoulos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menelaos N.","family":"Manoussakis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,6,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19810801)48:3<745::AID-CNCR2820480314>3.0.CO;2-7"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19870901)60:5<1000::AID-CNCR2820600514>3.0.CO;2-4"},{"key":"e_1_2_6_4_2","first-page":"7219","article-title":"Effects of retinoic acid on morphological features and biological markers of a neoplastic human salivary intercalated duct cell line in culture","volume":"48","author":"Azuma M","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(88)90067-5"},{"key":"e_1_2_6_6_2","first-page":"559","article-title":"Explant culture of human submandibular gland epithelial cells: evidence of epithelial origin","volume":"52","author":"Sens DA","year":"1985","journal-title":"Laboratory Invest"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02631121"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/j.1432-0436.1993.tb01597.x","article-title":"Characterization of growth and differentiation of normal human submandibular gland epithelial cells in a serum\u2010free medium","volume":"54","author":"Okura M","year":"1994","journal-title":"Differentiation"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.1041550202"},{"key":"e_1_2_6_10_2","first-page":"191","article-title":"Study of ductal epithelial cell component of human labial salivary glands in vitro","volume":"12","author":"Lamey PJ","year":"1984","journal-title":"J Biol Buccale"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1989.tb00765.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1053\/berh.1999.0078"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1994.1123"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"3109","DOI":"10.4049\/jimmunol.135.5.3109","article-title":"Production of interleukin 2 (IL 2) by salivary gland lymphocytes in Sj\u00f6gren's syndrome","volume":"135","author":"Fox RI","year":"1985","journal-title":"J Immunol"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/34.4.326"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/34.3.226"},{"key":"e_1_2_6_17_2","first-page":"475","article-title":"Epithelial HLA\u2010DR expression in T lymphocyte subsets in salivary glands in Sj\u00f6gren's syndrome","volume":"61","author":"Lindahl G","year":"1985","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780290908"},{"key":"e_1_2_6_19_2","first-page":"677","article-title":"HLA\u2010DR expression by labial minor salivary gland tissues in Sj\u00f6gren's syndrome","volume":"45","author":"Moutsopoulos HM","year":"1986","journal-title":"Arthritis Rheum"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199902)42:2<229::AID-ANR4>3.0.CO;2-X"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2001.01500.x"},{"key":"e_1_2_6_22_2","first-page":"693","article-title":"C\u2010myc mRNA expression in minor salivary glands of patients with Sj\u00f6gren's syndrome","volume":"19","author":"Skopouli FN","year":"1992","journal-title":"J Rheumatol"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00918149"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780390510"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1998.00705.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.3109\/08916939409014674"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780360309"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.5.3107"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(95)00170-F"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.2001.0524"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-0436.1993.tb00637.x"},{"key":"e_1_2_6_32_2","first-page":"(in press)","article-title":"CD40 on salivary gland epithelial cells: high constitutive expression by cultured cells from Sj\u00f6gren's syndrome patients indicating their intrinsic activation","author":"Dimitriou ID","year":"2001","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02624152"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02628416"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12540422"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(83)90115-5"}],"container-title":["European Journal of Oral Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0722.2002.00152.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0722.2002.00152.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0722.2002.00152.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T22:20:28Z","timestamp":1697581228000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0722.2002.00152.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,2]]},"references-count":35,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,2]]}},"alternative-id":["10.1034\/j.1600-0722.2002.00152.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0722.2002.00152.x","archive":["Portico"],"relation":{},"ISSN":["0909-8836","1600-0722"],"issn-type":[{"value":"0909-8836","type":"print"},{"value":"1600-0722","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,2]]},"assertion":[{"value":"2003-06-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}