{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T19:12:48Z","timestamp":1782933168832,"version":"3.54.5"},"reference-count":20,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2002,9,18]],"date-time":"2002-09-18T00:00:00Z","timestamp":1032307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["European J Oral Sciences"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:p>The role of matrix metalloproteinases (MMPs) in the breakdown of pulp tissue of teeth with severe caries has not yet been directly elucidated. This study was to determine the levels of selected MMPs and the overall gelatinolytic activity in clinically healthy and inflamed human dental pulps of 29 healthy subjects, aged 10\u201319\u2003yr. Seventeen pulps were collected from subjects diagnosed with symptomatic pulpitis, and 18 control pulps were obtained from 12 subjects following premolar extraction for orthodontic reasons. The levels of MMP\u20101, MMP\u20102, MMP\u20103 and MMP\u20109 were determined with enzyme\u2010linked immunosorbent assay. Densitometric analysis of gelatin zymograms was used to assay gelatinolytic activity in pulp supernatants. The MMP\u20101 levels were below the detection limit for both groups. Levels of MMP\u20102 and MMP\u20103 were significantly lower in symptomatic vs. clinically healthy pulps. In contrast, levels of MMP\u20109 in inflamed pulps were significantly higher than those recorded in clinically normal pulps. The overall gelatinolytic activity was elevated in inflamed pulps compared with healthy counterparts. Further, the gelatinolytic activity was positively correlated with MMP\u20109 levels. The data obtained suggest a key role of MMP\u20109 in the breakdown of inflamed human dental pulp tissue.<\/jats:p>","DOI":"10.1034\/j.1600-0722.2002.21347.x","type":"journal-article","created":{"date-parts":[[2003,3,11]],"date-time":"2003-03-11T19:47:24Z","timestamp":1047412044000},"page":"353-357","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Matrix metalloproteinase levels and gelatinolytic activity in clinically healthy and inflamed human dental pulps"],"prefix":"10.1111","volume":"110","author":[{"given":"Heloisa","family":"Gusman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronaldo B.","family":"Santana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Zehnder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,9,18]]},"reference":[{"key":"e_1_2_18_2_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090020301"},{"key":"e_1_2_18_3_2","first-page":"1","article-title":"Bacteriological studies on deep areas of carious dentine","volume":"32","author":"Edwardsson S","year":"1974","journal-title":"Odontol Rev Suppl"},{"key":"e_1_2_18_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-9657.1987.tb00619.x"},{"key":"e_1_2_18_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/10454411990100010101"},{"key":"e_1_2_18_6_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1993.64.5s.474"},{"key":"e_1_2_18_7_2","doi-asserted-by":"publisher","DOI":"10.1177\/08959374940080022701"},{"key":"e_1_2_18_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1999.tb07697.x"},{"key":"e_1_2_18_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(06)80070-1"},{"key":"e_1_2_18_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0099-2399(06)81717-6"},{"key":"e_1_2_18_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004770-200204000-00013"},{"key":"e_1_2_18_12_2","first-page":"435","article-title":"Quantitative determination of albumin, lysozyme, and amylase. 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