{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:44:53Z","timestamp":1789911893380,"version":"4.0.1"},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":450,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Research"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:p>Tyrosinase related protein\u20101 (TRP\u20101) is a melanocyte\u2010specific gene product involved in eumelanin synthesis. Mutation in the <jats:italic>Tyrp1<\/jats:italic> gene is associated with brown pelage in mouse and oculocutaneous albinism Type 3 in humans (OCA3). It has been demonstrated that TRP\u20101 expresses DHICA oxidase activity in the murine system. However, its actual function in the human system is still unclear. The study was designed to determine the effects of mutation at two <jats:italic>Typr1<\/jats:italic> alleles, namely the <jats:italic>Tyrp1<\/jats:italic><jats:sup><jats:italic>b<\/jats:italic><\/jats:sup> (brown) and <jats:italic>Tyrp1<\/jats:italic><jats:sup><jats:italic>b\u2010cj<\/jats:italic><\/jats:sup> (cordovan) compared with wild type <jats:italic>Tyrp1<\/jats:italic><jats:sup><jats:italic>B<\/jats:italic><\/jats:sup> (black) on melanocyte function and melanin biosynthesis. The most significant finding was that both of the <jats:italic>Tyrp1<\/jats:italic> mutations (i.e. brown expressing a point mutation and cordovan expressing decreased amount of TRP\u20101 protein) resulted in attenuation of cell proliferation rates. Neither necrosis nor apoptosis was responsible for the observed decrease in cell proliferation rates of the brown and cordovan melanocytes. Ultrastructural evaluation by electron microscopic analysis revealed that both mutations in <jats:italic>Tyrp1<\/jats:italic> affected melanosome maturation without affecting its structure. These observations demonstrate that mutation in <jats:italic>Tyrp1<\/jats:italic> compromised tyrosinase activity within the organelle. DOPA histochemistry revealed differences in melanosomal stages between black and brown melanocytes but not between black and cordovan melanocytes. There were no significant differences in tyrosine hydroxylase activities of tyrosinase and TRP\u20101 in wild type black, brown and cordovan melanocyte cell lysates. We conclude that mutations in <jats:italic>Tyrp1<\/jats:italic> compromise cell proliferation and melanosomal maturation in mouse melanocyte cultures.<\/jats:p>","DOI":"10.1034\/j.1600-0749.2000.130506.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T16:35:21Z","timestamp":1047486921000},"page":"337-344","source":"Crossref","is-referenced-by-count":35,"title":["Mutant Alleles at the <i>Brown<\/i> Locus Encoding Tyrosinase\u2010Related Protein\u20101 (TRP\u20101) Affect Proliferation of Mouse Melanocytes in Culture\t\t\t\t\t*"],"prefix":"10.1111","volume":"13","author":[{"given":"RANGAPRASAD","family":"SARANGARAJAN","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YANG","family":"ZHAO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"GEORGE","family":"BABCOCK","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JAMES","family":"CORNELIUS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. LYNN","family":"LAMOREUX","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RAYMOND E.","family":"BOISSY","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"container-title":["Pigment Cell Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0749.2000.130506.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0749.2000.130506.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0749.2000.130506.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T15:02:31Z","timestamp":1698591751000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0749.2000.130506.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,10]]}},"alternative-id":["10.1034\/j.1600-0749.2000.130506.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0749.2000.130506.x","archive":["Portico"],"relation":{},"ISSN":["0893-5785","1600-0749"],"issn-type":[{"value":"0893-5785","type":"print"},{"value":"1600-0749","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,10]]}}}