{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T13:47:06Z","timestamp":1725630426951},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2001,12,25]],"date-time":"2001-12-25T00:00:00Z","timestamp":1009238400000},"content-version":"vor","delay-in-days":450,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pigment Cell Research"],"published-print":{"date-parts":[[2000,10]]},"abstract":"<jats:p>In order to induce melanin production in mammalian cells with pigment disorders such as <jats:italic>albino<\/jats:italic> hair, a recombinant retrovirus containing the <jats:italic>mel<\/jats:italic> locus of <jats:italic>Streptomyces antibioticus<\/jats:italic> was constructed. The <jats:italic>S. antibioticus<\/jats:italic><jats:italic>mel<\/jats:italic> locus, which consists of the open reading frame (ORF)\u2010438 and the tyrosinase gene, was specifically derived by polymerase chain reaction (PCR) from <jats:italic>Streptomyces<\/jats:italic> plasmid pIJ702. The ORF\u2010438 is required for the transfer of copper to apotyrosinase, which is essential for tyrosinase enzymatic activity. The tyrosinase gene was inserted into the XhoI\/BamHI cloning site of the pLXSN retroviral vector to obtain pL<jats:italic>tyr<\/jats:italic>SN. An internal ribosome entry site (IRES) suitable for mammalian cell expression was obtained from the pLXIN retroviral vector by PCR. The ORF\u2010438 and IRES DNA fragments were inserted into the pL<jats:italic>tyr<\/jats:italic>SN vector to obtain the tyrosinase\u2010expression retroviral vector pL<jats:italic>mel<\/jats:italic>SN. The expression vector was amplified in murine PT67 packaging cells, where the ORF\u2010438 and tyrosinase genes were also co\u2010expressed as determined by reverse transcription\u2010PCR. In order to evaluate the vector's ability to restore pigment production in cells with a pigment disorder, <jats:italic>albino<\/jats:italic> \u2010mouse skins were histocultured and then infected with the pL<jats:italic>mel<\/jats:italic>SN retrovirus. Six days after infection, melanin granules were observed in \u223c60% of <jats:italic>albino<\/jats:italic>\u2010mouse hair follicles in the histocultured skin. These results demonstrated that the <jats:italic>S. antibioticus<\/jats:italic><jats:italic>mel<\/jats:italic> operon could express an active tyrosinase and produce melanin in the <jats:italic>albino<\/jats:italic>\u2010mouse hair follicles. This novel gene therapy approach, using a small and simple tyrosinase operon in a high\u2010expression vector, has a potentially wide application for therapy of pigment disorders in hair follicles.<\/jats:p>","DOI":"10.1034\/j.1600-0749.2000.130507.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T16:35:21Z","timestamp":1047486921000},"page":"345-351","source":"Crossref","is-referenced-by-count":8,"title":["A Novel Approach to Gene Therapy of <i>Albino<\/i> Hair In Histoculture with a Retroviral <i>Streptomyces<\/i> Tyrosinase Gene"],"prefix":"10.1111","volume":"13","author":[{"given":"MING","family":"ZHAO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"NORIMITSU","family":"SAITO","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"LINGNA","family":"LI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"EUGENE","family":"BARANOV","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"HIROFUMI","family":"KONDOH","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YUTAKA","family":"MISHIMA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MASANORI","family":"SUGIYAMA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"KENSEI","family":"KATSUOKA","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ROBERT M.","family":"HOFFMAN","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,12,25]]},"container-title":["Pigment Cell Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0749.2000.130507.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0749.2000.130507.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0749.2000.130507.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T15:02:26Z","timestamp":1698591746000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0749.2000.130507.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,10]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2000,10]]}},"alternative-id":["10.1034\/j.1600-0749.2000.130507.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0749.2000.130507.x","archive":["Portico"],"relation":{},"ISSN":["0893-5785","1600-0749"],"issn-type":[{"value":"0893-5785","type":"print"},{"value":"1600-0749","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,10]]}}}