{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T11:10:34Z","timestamp":1782472234408,"version":"3.54.5"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,12,2]],"date-time":"2003-12-02T00:00:00Z","timestamp":1070323200000},"content-version":"vor","delay-in-days":1400,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2000,2]]},"abstract":"Eruption of primary teeth has a great influence on the oral environment by providing suitable niches for bacterial colonization. The aim of the study was to investigate the composition of the subgingival microbiota of primary incisors, canines and molars in 40 systemically healthy children aged 4\u20135\u2003yr, chosen randomly. Subgingival plaque samples were taken from the mesiobuccal sites of primary incisors (61, 81), canines (53, 73) and molars (64, 84). The samples were cultured for bacterial isolation anaerobically and in 10% CO2<\/jats:sub> plus air using selective and non\u2010selective media. Forty\u2010one different microbial species were isolated. Gemella morbillorum<\/jats:italic> and Peptostreptococcus magnus<\/jats:italic> were statistically significantly more frequently detected in incisors while P. micros<\/jats:italic>, Streptococcus intermedius<\/jats:italic>, Bacteroides forsythus<\/jats:italic>, Fusobacterium nucleatum<\/jats:italic>, Prevotella loeschei<\/jats:italic>, P. melaninogenica<\/jats:italic> and Selenomonas sputigena<\/jats:italic> were more frequently detected in molars. The bacterial species S. constellatus<\/jats:italic>, G. morbillorum<\/jats:italic> and P. magnus<\/jats:italic> were isolated in greater numbers in incisors and P. micros<\/jats:italic>, S. intermedius<\/jats:italic>, Campylobacter concisus<\/jats:italic>, Bacteroides egertheii<\/jats:italic>, B. forsythus<\/jats:italic>, P. oralis<\/jats:italic> and S. sputigena<\/jats:italic> were isolated in greater numbers in molars, respectively. Cluster analysis revealed 4 clusters in which 6\u20137 bacterial species were elevated above mean levels. Cluster I was predominated by S. constellatus<\/jats:italic>, S. mitis<\/jats:italic>, S. sanguis<\/jats:italic>, G. morbillorum<\/jats:italic>, P. melaninogenica<\/jats:italic> and P. oralis<\/jats:italic>; cluster II was predominated by S. sanguis<\/jats:italic>, Actinomyces naeslundii<\/jats:italic>, Capnocytophaga gingivalis<\/jats:italic>, C. ochracea<\/jats:italic> and P. intermedia<\/jats:italic>; cluster III was predominated by S. mitis<\/jats:italic>, C. ochracea<\/jats:italic>, F. nucleatum<\/jats:italic>, P. loeschei<\/jats:italic>, P. melaninogenica<\/jats:italic> and P. oralis<\/jats:italic>; and finally cluster IV was predominated by S. sanguis<\/jats:italic>, C. gingivalis<\/jats:italic>, Veillonella parvula<\/jats:italic>, Campylobacter gracilis<\/jats:italic>, F. nucleatum<\/jats:italic> and P. intermedia.<\/jats:italic> The bacterial species S. constellatus<\/jats:italic>, P. micros<\/jats:italic>, Pseudoramibacter alactolyticus<\/jats:italic>, Eikenella corrodens<\/jats:italic> and F. nucleatum<\/jats:italic> were associated with non\u2010bleeding sites while S. intermedius<\/jats:italic>, C. concisus<\/jats:italic>, P. intermedia<\/jats:italic> and P. loescheii<\/jats:italic> were found more frequently in bleeding sites.<\/jats:p>","DOI":"10.1034\/j.1600-0765.2000.035001033.x","type":"journal-article","created":{"date-parts":[[2003,12,2]],"date-time":"2003-12-02T12:03:29Z","timestamp":1070366609000},"page":"33-41","source":"Crossref","is-referenced-by-count":32,"title":["Profile of subgingival microbiota in children with primary dentition"],"prefix":"10.1111","volume":"35","author":[{"given":"Joanna J.","family":"Kamma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthoula","family":"Diamanti\u2010Kipioti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mela","family":"Nakou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fotis J.","family":"Mitsis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,12,2]]},"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2000.035001033.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2000.035001033.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0765.2000.035001033.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T13:04:04Z","timestamp":1698584644000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0765.2000.035001033.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["10.1034\/j.1600-0765.2000.035001033.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0765.2000.035001033.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,2]]}}}