{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T17:15:59Z","timestamp":1775495759707,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2003,12,2]],"date-time":"2003-12-02T00:00:00Z","timestamp":1070323200000},"content-version":"vor","delay-in-days":1218,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2000,8]]},"abstract":"<jats:p>The primary objectives of this study were to investigate the prevalence of 8 putative periodontal pathogens in subjects with early\u2010onset periodontitis (EOP) and to evaluate the microbial differences between localized and generalized forms of this periodontal disease condition. Thirty\u2010one females and 11 males with a mean age of 30.3 (s.d. 4.0) years were examined. Seventeen subjects had generalized (GEOP) and 25 had localized early\u2010onset periodontitis (LEOP). Subgingival plaque samples were assayed using PCR which provided subject prevalence data for the pathogens; <jats:italic>Bacteroides forsythus<\/jats:italic> 78.6%, <jats:italic>Treponema denticola<\/jats:italic> 88.1%, <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> 19.0%, <jats:italic>Porphyromonas gingivalis<\/jats:italic> 16.7%, <jats:italic>Prevotella intermedia<\/jats:italic> 40.4%, <jats:italic>Prevotella nigrescens<\/jats:italic> 61.9%, <jats:italic>Eikenella corrodens<\/jats:italic> 42.3% and <jats:italic>Campylobacter rectus<\/jats:italic> 92.8%. Only 3 healthy sites harbored one or more of these periodontal pathogens. Seven of the 8 subjects positive for <jats:italic>A. actinomycetemcomitans<\/jats:italic> had LEOP. <jats:italic>P. intermedia<\/jats:italic> was present in 58.8% of GEOP compared with 28% of LEOP subjects (<jats:italic>p<\/jats:italic>=0.046). At 82.4% of GEOP sites <jats:italic>P. nigrescens<\/jats:italic> was present while this bacteria was detected at 52% of LEOP (<jats:italic>p<\/jats:italic>=0.044). <jats:italic>P.<\/jats:italic><jats:italic>gingivalis<\/jats:italic> was isolated from 22.6% of females but no male subjects (<jats:italic>p<\/jats:italic>=0.084). <jats:italic>C. rectus<\/jats:italic> was recovered from all female subjects compared to 72.7% of males (<jats:italic>p<\/jats:italic>=0.014). <jats:italic>A. actinomycetemcomitans<\/jats:italic>(37.5%) and <jats:italic>C. rectus<\/jats:italic>(86.5%) were more frequently identified in non\u2010smokers compared to 7.6% and 68.8% of smokers, respectively (<jats:italic>p<\/jats:italic>&lt;0.05). Microbial associations coincided with the clinical division of the cases into LEOP and GEOP in 83% of the subjects.<\/jats:p>","DOI":"10.1034\/j.1600-0765.2000.035004232.x","type":"journal-article","created":{"date-parts":[[2003,12,2]],"date-time":"2003-12-02T12:02:50Z","timestamp":1070366570000},"page":"232-241","source":"Crossref","is-referenced-by-count":63,"title":["Prevalence of periodontal pathogens in localized and generalized forms of early\u2010onset periodontitis"],"prefix":"10.1111","volume":"35","author":[{"given":"Brian H.","family":"Mullally","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry","family":"Dace","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles E.","family":"Shelburne","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Larry F.","family":"Wolff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilson A.","family":"Coulter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,12,2]]},"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2000.035004232.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2000.035004232.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0765.2000.035004232.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,29]],"date-time":"2023-10-29T07:31:20Z","timestamp":1698564680000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0765.2000.035004232.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,8]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2000,8]]}},"alternative-id":["10.1034\/j.1600-0765.2000.035004232.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0765.2000.035004232.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,8]]}}}