{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:50:30Z","timestamp":1778658630631,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,3,25]],"date-time":"2002-03-25T00:00:00Z","timestamp":1017014400000},"content-version":"vor","delay-in-days":297,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2001,6]]},"abstract":"<jats:p>Tumor necrosis factor (TNF)\u2010\u03b1 is a potent proinflammatory cytokine that is able to induce tissue destruction and bone resorption. A G\u2010to\u2010A polymorphism at the \u2212308 position of the TNF\u2010\u03b1 promoter region was suggested to influence TNF\u2010\u03b1 production. We had previously shown that monocytes of patients with localized early\u2010onset periodontitis (EOP) secrete high levels of TNF\u2010\u03b1 compared to matched controls. The aim of the present study was to investigate the possible link between the \u2212308 polymorphism in the TNF\u2010\u03b1 gene and EOP. Genomic DNA was extracted from the blood of 64 individuals from 11 nuclear families with EOP. The TNF\u2010\u03b1 polymorphism at \u2212308 was assessed using allele\u2010specific polymerase chain reaction. 77% of the tested adolescents were found to have the G\/G genotype, and 23% had the A\/G genotype. In the diseased subjects, 81% were with G\/G genotype and 19% with A\/G genotype. The healthy children had 74% G\/G genotype, while 26% had A\/G genotype. The differences between the disease group and the healthy group were not statistically significant. In summary, the present results could not demonstrate any link between EOP and genetic polymorphism in the \u2212308 position of the TNF\u2010\u03b1 promoter.<\/jats:p>","DOI":"10.1034\/j.1600-0765.2001.360307.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T08:54:42Z","timestamp":1047459282000},"page":"183-186","source":"Crossref","is-referenced-by-count":42,"title":["Genetic polymorphism of the tumor necrosis factor (TNF)\u2010\u03b1 promoter region in families with localized early\u2010onset periodontitis"],"prefix":"10.1111","volume":"36","author":[{"given":"Lior","family":"Shapira","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayala","family":"Stabholz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Rieckmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niels","family":"Kruse","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,3,25]]},"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2001.360307.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2001.360307.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0765.2001.360307.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T18:43:29Z","timestamp":1700419409000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0765.2001.360307.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,6]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2001,6]]}},"alternative-id":["10.1034\/j.1600-0765.2001.360307.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0765.2001.360307.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,6]]}}}