{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:15:16Z","timestamp":1760886916398},"reference-count":0,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2002,3,25]],"date-time":"2002-03-25T00:00:00Z","timestamp":1017014400000},"content-version":"vor","delay-in-days":114,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2001,12]]},"abstract":"<jats:p>Differential gene expression was investigated in neutrophils stimulated with <jats:italic>N<\/jats:italic>\u2010formyl\u2010methionyl\u2010leucyl\u2010phenylalanine using RNA fingerprinting by arbitrarily primed polymerase chain reaction (RAP\u2010PCR). The cells were isolated from 3 groups of subjects: patients with generalized aggressive periodontitis (Aggressive\u2010P, <jats:italic>n<\/jats:italic>=6), generalized chronic periodontitis (Chronic\u2010P, <jats:italic>n<\/jats:italic>=6) and healthy controls (H, <jats:italic>n<\/jats:italic>=8). Our results show that 37 genes were upregulated, while 27 genes were down\u2010regulated in all Aggressive\u2010P neutrophils by using RAP\u2010PCR with 45 primer pairs. Reverse transcription\u2010PCR analyses revealed that mRNA levels were significantly different (<jats:italic>p<\/jats:italic>&lt;0.05) for heat shock transcription factor 4b (HSF4b) gene, Kruppel\u2010like zinc finger transcription factor 9 (Zf9) and muskelin genes. HSF4b was greater in neutrophils from Aggressive\u2010P compared to groups H and Chronic\u2010P. Zf9 and muskelin genes were lower in Aggressive\u2010P compared to the H groups, but no significant difference was noted compared to the Chronic\u2010P group. The control genes, IL\u20101\u03b2 and VEGF genes, were expressed at a significantly higher level in Aggressive\u2010P and Chronic\u2010P than H (<jats:italic>p<\/jats:italic>&lt;0.01, <jats:italic>p<\/jats:italic>&lt;0.05). In conclusion, the RAP\u2010PCR technique used in this study enabled us to identify 3 Aggressive\u2010P related genes, which had not been reported previously. Neutrophil functions in Aggressive\u2010P patients are suggested to be altered by regulatory factors of the immune system including HSF4b (transcription factor), Zf9 (activator of TGF\u2010\u03b2) and muskelin (cellular adhesion).<\/jats:p>","DOI":"10.1034\/j.1600-0765.2001.360607.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T08:55:12Z","timestamp":1047459312000},"page":"390-397","source":"Crossref","is-referenced-by-count":16,"title":["Differential gene expression in neutrophils from patients with generalized aggressive periodontitis"],"prefix":"10.1111","volume":"36","author":[{"given":"Takehiko","family":"Kubota","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiya","family":"Morozumi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kunihiko","family":"Shimizu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noriko","family":"Sugita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuo","family":"Kobayashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromasa","family":"Yoshie","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,3,25]]},"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2001.360607.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2001.360607.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0765.2001.360607.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T18:45:50Z","timestamp":1700419550000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0765.2001.360607.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2001,12]]}},"alternative-id":["10.1034\/j.1600-0765.2001.360607.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0765.2001.360607.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,12]]}}}