{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:29:09Z","timestamp":1791217749580,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2002,5,28]],"date-time":"2002-05-28T00:00:00Z","timestamp":1022544000000},"content-version":"vor","delay-in-days":57,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:p>Interleukin\u20108 (IL\u20108) has been implicated in a wide variety of diseases. Previous studies have demonstrated the expression of IL\u20108 in periodontal tissues, yet little is known about the exact source(s), mechanisms and factors involved in gingival expression of IL\u20108. Additionally, nothing is known about the presence and distribution of IL\u20108 receptors (IL\u20108R) in gingival cells. Therefore it was hypothesized that, <jats:italic>in vivo<\/jats:italic>, periodontal pathogens induce IL\u20108 expression from gingival keratinocytes (GK) which enhances leukocyte, microvascular endothelial cell (MVEC) and GK migration via specific IL\u20108 receptors present on these cells. The objective of the present study was to determine the distribution of IL\u20108 and IL\u20108R in gingival tissues and cultured human GK <jats:italic>in vitro<\/jats:italic>. Standard immunohistochemical and immunocytochemical techniques were utilized in order to localize IL\u20108 and its receptors CXCR\u20101 and CXCR\u20102 in archival gingival specimens (eight periodontitis and four non\u2010inflamed controls) and in cultured gingival keratinocytes. It was demonstrated that, <jats:italic>in vivo<\/jats:italic>, IL\u20108 and IL\u20108R were present in gingival epithelium, MVEC and leukocytes. <jats:italic>In vitro<\/jats:italic> studies verified the above results, by showing expression of IL\u20108 and IL\u20108R in cultured gingival keratinocytes. It is concluded that IL\u20108 and IL\u20108 receptors are expressed in gingival epithelium both <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>. This new evidence indicates that epithelium plays a critical role in the host defense against invading pathogens and that keratinocytes can actively respond to IL\u20108 and other host cytokines, via specific receptors.<\/jats:p>","DOI":"10.1034\/j.1600-0765.2002.00024.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T08:55:40Z","timestamp":1047459340000},"page":"154-160","source":"Crossref","is-referenced-by-count":30,"title":["Localization of the chemokine interleukin\u20108 and interleukin\u20108 receptors in human gingiva and cultured gingival keratinocytes"],"prefix":"10.1111","volume":"37","author":[{"given":"Andreas","family":"Sfakianakis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles E.","family":"Barr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don L.","family":"Kreutzer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,5,28]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1993.tb00220.x"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00191.x"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.1997.tb00189.x"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v19.i1.10"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1281554"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12478128"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.62.9.4005-4014.1994"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9610(97)00165-7"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1996.0215"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1995.tb00158.x"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1998.69.8.865"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1993.tb02114.x"},{"key":"e_1_2_5_14_2","first-page":"42","article-title":"Detection of inflammatory cytokine messenger RNA (mRNA)\u2010expressing cells in human inflamed gingiva by combined in situ hybridization and immunohistochemistry","volume":"76","author":"Matsuki Y","year":"1992","journal-title":"Immunology"},{"key":"e_1_2_5_15_2","doi-asserted-by":"crossref","first-page":"2587","DOI":"10.4049\/jimmunol.155.5.2587","article-title":"Regulation of the expression of IL\u20108 receptor A\/B by IL\u20108: possible functions of each receptor","volume":"155","author":"Chuntharapai A","year":"1995","journal-title":"J Immunol"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2002.00024.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1034%2Fj.1600-0765.2002.00024.x","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0765.2002.00024.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T01:35:21Z","timestamp":1697506521000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0765.2002.00024.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4]]},"references-count":14,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2002,4]]}},"alternative-id":["10.1034\/j.1600-0765.2002.00024.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0765.2002.00024.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,4]]}}}