{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T17:40:51Z","timestamp":1697305251518},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2003,4,30]],"date-time":"2003-04-30T00:00:00Z","timestamp":1051660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2003,6]]},"abstract":"<jats:p><jats:bold>Objectives:\u2002<\/jats:bold> In 34 patients with chronic periodontitis, the presence of IgA, IgG, and IgG subclass serum antibodies against recombinant PrtC (<jats:italic>r<\/jats:italic>PrtC) of <jats:italic>Porphyromonas gingivalis<\/jats:italic> was assessed by immunoblot analysis 24\u2003months after therapy.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>  <jats:italic>r<\/jats:italic>PrtC was produced from <jats:italic>P.\u2003gingivalis<\/jats:italic> ATTC 33277 using the plasmid pGEX\u20102T. In addition, intraoral colonization with <jats:italic>P.\u2003gingivalis<\/jats:italic> was detected by PCR in subgingival plaque and swab samples from buccal mucosae, tonsils and tongue at baseline, 10\u2003d, and 3, 6, 9, 12, 18, and 24\u2003months.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> All patients were found to harbor <jats:italic>P.\u2003gingivalis<\/jats:italic> in the oral cavity at least once during the observation period. The identified antibody responses against the <jats:italic>r<\/jats:italic>PrtC of <jats:italic>P.\u2003gingivalis<\/jats:italic> were IgA (97%, i.e. 33\/34 patients) and IgG (100%, i.e. 34\/34), with an IgG subclass distribution of IgG2 (65%, i.e. 22\/34 patients)\u2003&gt;\u2003IgG3 (47%, i.e. 16\/34)\u2003&gt;\u2003IgG1 (38%, i.e. 13\/34)\u2003&gt;\u2003IgG4 (29%, i.e. 10\/34). Anti\u2010<jats:italic>r<\/jats:italic>PrtC IgA and IgG antibody reactivity was found in all but one patients (anti\u2010<jats:italic>r<\/jats:italic>PrtC IgA negative), who tested negative for <jats:italic>P.\u2003gingivalis<\/jats:italic> at all of the assessed intraoral sites for at least 6\u2003months before sera collection. There was no association between IgG subclass reactivity against the <jats:italic>r<\/jats:italic>PrtC of <jats:italic>P.\u2003gingivalis<\/jats:italic> and progression of periodontal attachment loss.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> The results indicated that anti\u2010<jats:italic>r<\/jats:italic>PrtC IgA and IgG antibodies may serve as an indicator for past or present intraoral colonization with <jats:italic>P.\u2003gingivalis<\/jats:italic>.<\/jats:p>","DOI":"10.1034\/j.1600-0765.2003.01405.x","type":"journal-article","created":{"date-parts":[[2009,9,11]],"date-time":"2009-09-11T13:07:07Z","timestamp":1252674427000},"page":"276-281","source":"Crossref","is-referenced-by-count":5,"title":["Serum antibody reactivity against recombinant PrtC of <i>Porphyromonas gingivalis<\/i> following periodontal therapy"],"prefix":"10.1034","volume":"38","author":[{"given":"T.","family":"Beikler","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Ehmke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Wittstock","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Schmidt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H.","family":"Karch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. 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