{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:12:53Z","timestamp":1761707573631},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,1,28]],"date-time":"2003-01-28T00:00:00Z","timestamp":1043712000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J of Periodontal Research"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:p>Lipopolysaccharide (LPS) was recognized by CD14, which may be an important mediator in the deleterious effects of LPS on the periodontal destruction. To investigate the roles of CD14 molecules on LPS\u2010induced soft tissue inflammation and bone destruction, the tissues of CD14\u2010deficient mice were examined histopathologically following a local injection of either <jats:italic>Salmonella minnesota<\/jats:italic> or <jats:italic>Porphyromonas gingivalis<\/jats:italic> LPS. In the first group, 12 mice received a local injection of 500\u2003\u00b5g of purified <jats:italic>P.\u2003gingivalis<\/jats:italic> LPS and six mice were injected with saline to the calvaria as controls. In the second group 13 mice were injected subcutaneously on the laterally abdominal skin with 50\u2003\u00b5g of <jats:italic>S.\u2003minnesota<\/jats:italic> LPS and three mice were injected with PBS. Mice were sacrificed at day\u20035. After histological preparation, the tissue sections of calvaria and soft tissue specimen were stained with tartrate\u2010resistant acid phosphatase (TRAP) marker for osteoclast and macrophage. The soft tissue sections were also stained with hematoxylin &amp; eosin (H&amp;E). Resorption surface and osteoclast index were measured to quantify bone resorption. Necrotic area and inflammatory cell numbers were estimated to assess the situation of local inflammation. Our results indicated that LPS\u2010induced bone resorption is inhibited in CD14\u2010deficient mice. An increase in the number of total inflammatory cells was noticed in both CD14\u2010deficient mice and wild\u2010type mice; however, the cell numbers were less in CD14\u2010deficient mice than those in wild\u2010type mice (two\u2010 to three\u2010fold decrease). Therefore, we conclude that the LPS\u2010stimulated bone resorption is mainly via CD14 receptor but the LPS\u2010induced soft tissue inflammation appears to be partially dependent on the receptor.<\/jats:p>","DOI":"10.1034\/j.1600-0765.2003.01617.x","type":"journal-article","created":{"date-parts":[[2003,3,12]],"date-time":"2003-03-12T08:56:15Z","timestamp":1047459375000},"page":"36-43","source":"Crossref","is-referenced-by-count":10,"title":["Effects of CD14 receptors on tissue reactions induced by local injection of two gram\u2010negative bacterial lipopolysaccharides"],"prefix":"10.1111","volume":"38","author":[{"given":"Cheng\u2010Yang","family":"Chiang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Earl","family":"Fu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E\u2010Chin","family":"Shen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hsien\u2010Chung","family":"Chiu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,1,28]]},"reference":[{"key":"e_1_2_15_2_2","first-page":"114","article-title":"The predominant cultivable microflora of advanced periodontitis","volume":"85","author":"Slots J.","year":"1997","journal-title":"Scan J Dent Res"},{"key":"e_1_2_15_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1985.tb01348.x"},{"key":"e_1_2_15_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199002083220606"},{"key":"e_1_2_15_5_2","unstructured":"LindheJ KarringL LangNP.Clinical periodontology and implant dentistry: plaque\u2010associated periodontal disease 3rd edn.1997: chapter 5 189\u2013225."},{"key":"e_1_2_15_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(87)90106-3"},{"key":"e_1_2_15_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.1990.tb02338.x"},{"key":"e_1_2_15_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1993.tb01059.x"},{"key":"e_1_2_15_9_2","unstructured":"JanewayC TraversAJr P.Immunobiology: the immune system in health and disease 2nd edn.1996: Chapter 9 10\u201319."},{"key":"e_1_2_15_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1993.tb02113.x"},{"key":"e_1_2_15_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.1.295-301.1991"},{"key":"e_1_2_15_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(83)90014-9"},{"key":"e_1_2_15_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1990.tb00422.x"},{"key":"e_1_2_15_14_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113852"},{"key":"e_1_2_15_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.145.10.3297"},{"key":"e_1_2_15_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.1991.tb01639.x"},{"key":"e_1_2_15_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.64.7.2371-2380.1996"},{"key":"e_1_2_15_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0952-7915(91)90082-C"},{"key":"e_1_2_15_19_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.164.3.777"},{"key":"e_1_2_15_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1698311"},{"key":"e_1_2_15_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(08)60502-7"},{"key":"e_1_2_15_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(93)90212-4"},{"key":"e_1_2_15_23_2","doi-asserted-by":"crossref","first-page":"5868","DOI":"10.4049\/jimmunol.152.12.5868","article-title":"Recombinant soluble CD14 inhibits LPS\u2010induced TNF\u2010\u03b1 production by cells in whole blood","volume":"152","author":"Haziot A","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_15_24_2","doi-asserted-by":"crossref","first-page":"4488","DOI":"10.1128\/iai.64.11.4488-4494.1996","article-title":"CD14\u2010mediated signal pathway of Porphyromonas gingivalis LPS in human gingival fibroblasts","volume":"64","author":"Watanabe A","year":"1996","journal-title":"Infect Immun"},{"key":"e_1_2_15_25_2","doi-asserted-by":"crossref","first-page":"5556","DOI":"10.4049\/jimmunol.150.12.5556","article-title":"Neutrophil CD14: biochemical properties and role in the secretion of TNF\u2010\u03b1 in response to LPS","volume":"150","author":"Haziot A","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_15_26_2","doi-asserted-by":"crossref","first-page":"3513","DOI":"10.1128\/iai.65.9.3513-3519.1997","article-title":"Porphyromonas gingivalis LPS\u2010stimulated bone resorption via CD14 is inhibited by broad\u2010spectrum antibodies","volume":"65","author":"Miyata Y","year":"1997","journal-title":"Infect Immun"},{"key":"e_1_2_15_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90490-5"},{"key":"e_1_2_15_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119679"},{"key":"e_1_2_15_29_2","doi-asserted-by":"crossref","first-page":"14147","DOI":"10.1016\/S0021-9258(18)98655-2","article-title":"IL\u20101 receptor antagonist competitively inhibits the binding of IL\u20101 to the type II IL\u20101 receptor","volume":"266","author":"Granowitz EV","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_15_30_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.4.931"},{"key":"e_1_2_15_31_2","doi-asserted-by":"crossref","first-page":"3829","DOI":"10.4049\/jimmunol.151.7.3829","article-title":"Lipopolysaccharide\u2010induced protein tyrosine phosphorylation in human macrophages is mediated by CD14","volume":"151","author":"Weinstein SL","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_15_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80254-X"},{"key":"e_1_2_15_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650020617"},{"key":"e_1_2_15_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/jbmr.5650100217"},{"key":"e_1_2_15_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9896(1998110)186:3<281::AID-PATH200>3.0.CO;2-7"},{"key":"e_1_2_15_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(73)90126-X"},{"key":"e_1_2_15_37_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1972.43.5.270"},{"key":"e_1_2_15_38_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1974.45.9.672"},{"key":"e_1_2_15_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.67.8.4231-4236.1999"},{"key":"e_1_2_15_40_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115106"},{"key":"e_1_2_15_41_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo.136.4.7534699"},{"key":"e_1_2_15_42_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.13.040195.002253"},{"key":"e_1_2_15_43_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.181.3.857"},{"key":"e_1_2_15_44_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2402637"},{"key":"e_1_2_15_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.21.9930"},{"key":"e_1_2_15_46_2","doi-asserted-by":"crossref","first-page":"4422","DOI":"10.4049\/jimmunol.158.9.4422","article-title":"CD14\u2010dependent and CD14\u2010independent signaling pathways in murine macrophages from normal and CD14 knockout mice stimulated with lipopolysaccharide or taxol","volume":"158","author":"Perera PY","year":"1997","journal-title":"J Immunol"},{"key":"e_1_2_15_47_2","doi-asserted-by":"crossref","first-page":"3215","DOI":"10.1128\/IAI.67.7.3215-3220.1999","article-title":"Moesin functions as a lipopolysaccharide receptor on human monocytes","volume":"67","author":"Tohme ZN","year":"1999","journal-title":"Infect Immun"},{"key":"e_1_2_15_48_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj3200589"},{"key":"e_1_2_15_49_2","first-page":"433","article-title":"The production of TNF\u2010\u03b1 and IL\u20108 in whole blood assays is differently regulated upon activation by LPS","volume":"392","author":"Cavaillon JM","year":"1995","journal-title":"Prog Clin Biol Res"}],"container-title":["Journal of Periodontal Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1034\/j.1600-0765.2003.01617.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T11:25:31Z","timestamp":1697196331000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1034\/j.1600-0765.2003.01617.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,1,28]]},"references-count":48,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2003,2]]}},"alternative-id":["10.1034\/j.1600-0765.2003.01617.x"],"URL":"https:\/\/doi.org\/10.1034\/j.1600-0765.2003.01617.x","archive":["Portico"],"relation":{},"ISSN":["0022-3484","1600-0765"],"issn-type":[{"value":"0022-3484","type":"print"},{"value":"1600-0765","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,1,28]]}}}